A Study on the Optimum Composition of Rose Bengal Reagent using Phase Transfer Catalyst

상이동 촉매 기법(phase transfer catalyst)을 이용한 rose bengal 시약의 최적 조성에 관한 연구

  • 오수진 (순천향대학교 법과학대학원) ;
  • 차원진 (순천향대학교 법과학대학원) ;
  • 최다운 (순천향대학교 법과학대학원) ;
  • 홍성욱 (순천향대학교 법과학대학원)
  • Received : 2018.06.08
  • Accepted : 2018.06.22
  • Published : 2018.07.28


The rose bengal reagent, based on the phase transfer catalysis technique, is a calcium-targeting reagent that forms an insoluble salt that does not dissolve in a neutral or basic solution. It is expected to be effective in developing a latent fingerprint in a wet sample. However, many previous studies did not observe the fluorescence of the developed fingerprints, nor were the proposed methods of producing the reagents the optimal develop conditions. The aim of this study was to investigate the optimum reagent composition of rose bengal by varying the concentration of rose bengal dye and phase transfer catalyst based on maximum emission fluorescence of rose bengal. As a result, it was confirmed that rose bengal and surfactant concentration were the most effective when 0.01M: 0.008M, respectively.


Rose Bengal;PTC;TBI


  1. M. J. Choi, W. S. Park, M. K. Kim, C. Jeon, and S. W. Park, "A study on oil-contaminated fingerprints developing," Koanal, Vol.23, No.1, pp.89-96, 2010.
  2. R. F. Becker, The Underwater Crime Scene: Underwater Crime Investigative Techniques, Charles C Thomas Pub Ltd, Springfield, Ill, 1995.
  3. S. M. Bleay and V. G. Sears, Fingerprint Source Book: manual of development techniques, GOV.UK., 2012.
  4. J. Salama, S. B. Aumeer-Donovan, C. J. Lennard, and C. P. Roux, "Evaluation of the fingermark reagent oil red O as a possible replacement for physical developer," J. Forensic Identif, Vol.58, No.2, pp.203-237, 2008.
  5. M. Trapecar, "Finger marks on glass and metal surfaces recovered from stagnant water," Egypt. J. Forensic Sci., Vol.2, pp.48-53, 2012.
  6. R. Rohatgi and A. K. Kapoor, "Development of latent fingerprints on wet non-porous surfaces with SPR based on basic fuchsin dye," Egypt. J. Forensic Sci., Vol.6, pp.79-184, 2016.
  7. R. S. Ramotowski, R. S. Ramotowski, H. C. Lee, and R. E. Gaensslen, Lee and Gaensslen's advances in fingerprint technology, 3rd ed., CRC Press, Boca Raton, FL, 2013.
  8. J. Kaur, G. S. Sodhi, and S. Nath, "The application of phase transfer catalysis to fingerprint detection," Sci. Justice., Vol.36, pp.267-269, 1996.
  9. O. P. Jasuja, G. D. Singh, and G. S. Sodhi, "Development of Latent Fingerprints on the Sticky Side of Adhesive Tapes: Phase Transfer Catalyst-Based Formulation," Can. Soc. Forensic Sci. J., Vol.40, pp.1-13, 2007.
  10. O. P. Jasuja, P. Kumar, and G. Singh, "Development of latent fingermarks on surfaces submerged in water: Optimization studies for phase transfer catalyst (PTC) based reagents," Sci. Justice J. Forensic Sci., Vol.55, No.5, pp.335-342, 2015.
  11. R. C. Kapoor, M. K. Jain, and V. N. Mishra, "Fluorescence and absorption spectra of Rosebengal dye in the presence of surfactants," J. Lumin, Vol.22, pp.429-439, 1981.
  12. Je-Seol Yu, So-Young Jeo, Kyu-Yeon Kim, Ji-Yeon Kim, Chae-Won Kim, and Jake Jang, "Estimation of Fingerprint Image Quality in Accordance with Photographing Conditions," The Korea Contents Society, Vol.17, No.6, pp.287-295, 2017.
  13. J. Moore, S. Bleay, J. Deans, and N. NicDaeid, "Recovery of Fingerprints from Arson Scenes: part2-Fingerprints in Blood," Journal of Forensic Identification, pp.83-108, 2008.
  14. C. Champod, C. J. Lennard, P. Margot, and M. Stoilovic, Fingerprints and Other Ridge Skin Impressions, 1 edition, CRC Press, Boca Raton, FL, 2004.