Validation of an analytical method of oxyresveratrol for standardization of Mulberry (Morus alba L.) branch extract as a functional ingredient

상지추출물의 기능성원료 표준화를 위한 지표성분 옥시레스베라트롤 분석법 검증

  • Jeon, Young-Hee (Department of Food Science & Nutrition, Daegu Catholic University) ;
  • Choi, Sang-Won (Department of Food Science & Nutrition, Daegu Catholic University)
  • 전영희 (대구가톨릭대학교 식품영양학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과)
  • Received : 2018.06.18
  • Accepted : 2018.07.26
  • Published : 2018.08.31


An HPLC method was developed to quantitate a marker, oxyresveratrol (ORT), for the standardization of mulberry branch extracts (MBE) as a functional ingredient. HPLC was performed on a $C_{18}$ column with a gradient elution using 0.05% $H_3PO_4$ and acetonitrile at a flow rate of 0.8 mL/min, and detected at 320 nm. The HPLC method was validated according to Korea Food and Drug Administration (KFDA) guideline of analytical procedures with respect to specificity, linearity, accuracy and precision. Calibration curve of ORT showed high linearity ($R^2=1$), and limits of detection and quantification were 0.3 and $1.0{\mu}g/mL$, respectively. Relative standard deviation values from intra-and inter-day precision were less than 3.52 and 4.70%, respectively. Recovery rate ranged from 97.64% to 103.69%, and ORT content in MBE was approximately 3.78%. These results suggest that the HPLC method developed for the analysis of ORT in MBE is simple, efficient, and could contribute to the quality control of MBE.


Supported by : 대구가톨릭대학교


  1. Ahn EY, Lee JM, Jeon YH, Choi SW, Kim EJ. Anti-diabetic effects of mulberry (Morus alba L.) branches and oxyresveratrol in streptozotocin-induced diabetic mice. Food Sci. Biotechnol. 26: 1693-1702 (2017)
  2. Chan EWC, Lye PY, Wong SK. Phytochemistry, pharmacology, and clinical trials of Morus alba. Chinese J. Nat. Med. 14: 17-30 (2016)
  3. Chang LW, Juang LJ, Wang BS, Wang MY, Tai HM, Hung WJ, Chen YJ, Huang MH. Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food Chem. Toxicol. 49: 785-790 (2011)
  4. Choi SW, Jang YJ, Lee YJ, Leem HH, Kim EO. Analysis of functional constituents in mulberry (Morus alba L.) twigs by different cultivars, producing areas, and heat processings. Prev. Nutr. Food Sci. 18: 256-262 (2013)
  5. Choi SW, Lee YJ, Ha SB, Jeon YH, Lee DH. Evaluation of biological activity and analysis of functional constituents from different parts of mulberry (Morus alba L.) tree. J. Korean Soc. Food Sci. Nutr. 44: 823-831 (2015)
  6. Chuanasa T, Phromjai J, Lipipun V, Likhitwitayawuid K, Suzuki M, Pramyothin P, Hattori M, Shiraki K. Anti-herpes simplex virus (HSV-1) activity of oxyresveratrol derived from thai medicinal plant: Mechanism of action and therapeutic efficacy on cutaneous HSV-1 infection in mice. Antivir. Res. 80: 62-70 (2008)
  7. Chung KO, Kim BY, Lee MH, Kim YR, Chung HY, Park JH, Moon JO. In vitro and in vivo anti-inflammatory effect of oxyresveratrol from Morus alba L. J. Pharm. Pharmcol. 55: 1695-1700 (2003)
  8. Fang SC, Hsu CL, Yen GC. Anti-inflammatory effects of phenolic compounds isolated from the fruits of Artocarpus heterophyllus. J. Agr. Food Chem. 56: 4463-4468 (2008)
  9. Food Safety Korea. Guidelines for the recognition of functional ingredient. Accessed Dec. 30, 2016.
  10. Guo C, Li R, Zheng N, Xu L, Liang T, He Q. Anti-diabetic effect of Ramulus mori polysaccharides, isolated from Morus alba L. on STZ-diabetic mice through blocking inflammatory response and attenuating oxidative stress. Int. Immunopharmacol. 16: 93-99 (2013)
  11. Hur J. Donguibogam, Dongeuhak Institute. Ryogang Pub. Co., Seoul, Korea, pp. 2803-2805 (1994)
  12. Hwang DH, Jo SP, Lee JY, Kim JK, Kim KH, Lim YH. Antihyperlipidaemic effects of oxyresveratrol containing Ramulus mori ethanol extract in rats fed a high-cholesterol diet. J. Functional Foods 19: 353-362 (2015)
  13. Kanchanapoom T, Suga K, Kasai R, Yamasaki K, Kamel MS, Mohamed MH. Stilbene and 2-arylbenzofuran glucosides from the rhizomes of Schoenocaulon officinale. Chem. Pharm. Bull. 50: 863-865 (2002)
  14. KFDA. Korean Food and Drug Administration. Standards and specifications of health functional foods. Accessed Feb. 28, 2018.
  15. Lee SI. Mori Fructus. Bonchohak. Suseowon, Seoul, Korea. pp. 136-137 (1981)
  16. Li H, Cheng KW, Cho CH, He Z, Wang M. Oxyresveratrol as an antibrowning agent for cloudy apple juices and fresh-cut apples. J. Agr. Food Chem. 55: 2604-2610 (2007)
  17. Lorenz P, Roychowdhury S, Engelmann M, Wolf G, Horn TF. Oxyresveratrol and resveratrol are potent antioxidants and free radical scavengers: Effect on nitrosative and oxidative stress derived from microglial cells. Nitric Oxide 9: 64-76 (2003)
  18. Mouihate A, Horn TF, Pittman QJ. Oxyresveratrol dampens neuroimmune responses in vivo: A selective effect on TNF-alpha. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291: R1215-1221 (2006)
  19. Park SY, Jin BR, Lee YR, Kim YJ, Park JB, Jeon YH, Choi SW, Kwon OR. Postprandial hypoglycemic effects of mulberry twig and root bark in vivo and in vitro. J. Nutr. Health 49: 18-27 (2016)
  20. Park SY, Jin BR, Shin JH, Adisakwattana SC, Kwon OR. Standardized Mori ramulus extract improves insulin secretion and insulin sensitivity in C57BLKS/J db/db mice and INS-1 cells. Biomed Pharmacother. 92: 308- 315 (2017)
  21. Song UI. Medicinal Plant Experiment Station of Agricultural Research Center in Gyeongbuk, Korea. Illustrated book of medicinal plants. p. 120 (2000)
  22. Tan HY, Iris MY, Li ETS, Wang M. Oxyresveratrol supplementation to C57bl/6 mice fed with a high-fat diet ameliorates obesity-associated symptoms. Nutrients 9: 147 (2017)
  23. Ye F, Shen Z, Xie M. Alpha-glucosidase inhibition from a Chinese medical herb (Ramulus mori) in normal and diabetic rats and mice. Phytomedicine 9: 161-166 (2002)
  24. Zheng ZP, Cheng KW, Zhu Q, Wang XC, Lin ZX, Wang M. Tyrosinase inhibitory constituents from the roots of Morus nigra: A structure-activity relationship study. J. Agr. Food Chem. 58: 5368-5373 (2010)
  25. Zheng ZP, Tan HY, Wang M. Tyrosinase inhibition constituents from the roots of Morus australis. Fitoterapia 83: 1008-1013 (2012)