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Neuroprotective effects of cultured and fermented wild ginseng extracts on oxidative stress induced by hydrogen peroxide in PC12 cells

발효산삼배양근농축액의 산화방지 효과 및 과산화수소로 유발된 PC12 세포독성 보호효과

  • 최여옥 (바이오생명공학연구소) ;
  • 김유리 ((주)제이아이바이오팜) ;
  • 신승용 ((주)제이아이바이오팜) ;
  • 이재근 ((주)화진바이오코스메틱) ;
  • 김철중 ((주)화진바이오코스메틱) ;
  • 이예지 ((주)화진바이오코스메틱) ;
  • 강병주 ((주)제이아이바이오팜) ;
  • 김관수 ((주)제이아이바이오팜) ;
  • 최지은 ((주)제이아이바이오팜) ;
  • 한범석 (호서대학교 식품제약공학부)
  • Received : 2018.05.02
  • Accepted : 2018.07.25
  • Published : 2018.08.31

Abstract

Most neurodegenerative diseases are known to be influenced by oxidative stress. We investigated the anti-oxidative activity of the concentrate of fermented wild ginseng root culture (HLJG0701) containing ginsenosides Rg5 and Rk1. HLJG0701 showed effective DPPH and ABTS radical scavenging ability ($IC_{50}$: 16- and 4-fold dilution, respectively) and was inhibited dose-dependently by the $FeSO_4$-induced lipid peroxidation group (8- and 4-fold dilution: 2.3 and 1.5 nM, respectively). In MTT and LDH assays, 8-, 16-, 32- and 64-fold diluted HLJG0701 significantly increased cell viability by 70, 53, 35, and 26%, respectively. LDH released by HLJG0701 was reduced 1.3-fold with 8-fold diluted HLJG0701 compared to the $H_2O_2$-treated control. In addition, the inhibitory effect of HLJG0701 on oxidative stress in PC12 cells was confirmed by DCF-DA analysis (16-, 4-fold diluted HLJG0701: 50 and 68% ROS inhibition, respectively), TBARS (16- and 4-fold diluted HLJG0701: 50.7 and 46.5% inhibition, respectively), GPx (16- and 4-fold diluted HLJG0701: 133.3 and 227.3% release, respectively), and SOD analysis (16- and 4-fold diluted HLJG0701: 118.2 and 218.2% release, respectively). These results suggested that HLJG0701 protects neuronal cells by its anti-oxidative effects and hence can be a potential preventive material against neurodegenerative diseases.

Keywords

cultivated wild ginseng;oxidative stress;neurodegenerative diseases;ginsenoside Rg5;ginsenoside Rk1;PC12 cell

Acknowledgement

Supported by : 산업통상자원부

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