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Inhibition of adhesion and biofilm formation in Escherichia coli O157:H7 by diosmin

다이오스민(diosmin)에 의한 병원성 대장균 세포부착 및 생물막 형성 억제

  • Received : 2018.07.18
  • Accepted : 2018.08.13
  • Published : 2018.08.31

Abstract

Escherichia coli O157:H7 is one of the most common foodborne pathogens responsible for outbreaks of hemorrhagic colitis, which can lead to the life-threatening hemolytic-uremic syndrome. In this study, we identified phytochemicals that specifically inhibit the expression of LEE operon in E. coli O157:H7. Among phytochemicals, diosmin decreased the adherence of E. coli O157:H7 towards Caco-2 cells in vitro (p<0.01) and its biofilm formation activity (p<0.05). Quantitative RT-PCR analysis revealed that the transcripts of Ler-regulated genes and genes related to curli production were significantly reduced in the presence of diosmin. However, diosmin does not affect bacterial viability, indicating that the resistance rate to diosmin was remarkably low. Overall, these results provide significant insights into the development of a novel anti-infective agent that is different from conventional antibiotics.

Keywords

E. coli O157:H7;adhesion;biofilm;diosmin;antibiotic resistance

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Acknowledgement

Supported by : 한국식품연구원