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Immunomodulatory effect of bee pollen extract in macrophage cells

꿀벌 꽃가루 열수 추출물의 큰포식세포 면역활성 효과

  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 김이은 (공주대학교 식품공학과) ;
  • 조은지 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.05.16
  • Accepted : 2018.07.10
  • Published : 2018.08.31

Abstract

Activation of macrophages plays an important role in the host-immune system. In this study, we investigated the functional roles and related signaling mechanism of hot-water extracts of bee pollen (BPW) in RAW 264.7 macrophages. Since BPW did not exert cytotoxicity at concentrations ranging from 62.5 to $250{\mu}g/mL$ in macrophage cells, a concentration of $250{\mu}g/mL$ was used as the maximum dose of BPW throughout subsequent experiments. BPW increased inducible nitric oxide synthase-mediated nitric oxide production in a concentration-dependent manner. Additionally, BPW was found to induce macrophage activation by augmenting the expression of cell surface molecules (cluster of differentiation; CD80/86, and major histocompatibility complex; MHC class I/II) and production of pro-inflammatory cytokines (tumor necrosis $factor-{\alpha}$, interleukin-6, and $IL-1{\beta}$) through mitogen-activated protein kinase and nuclear $factor-{\kappa}B$ signaling pathways in RAW 264.7 macrophages. Taken together, our results indicate that BPW could potentially be used as an immunomodulatory agent.

Keywords

bee pollen;macrophage;immuno-modulatory activity;cytokine production;mitogen-activated protein kinase

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