DOI QR코드

DOI QR Code

Effect of bee pollen extract on activation of dendritic cells and induction of Th1 immune response

꿀벌 꽃가루 열수 추출물의 수지상 세포 활성화 및 Th1 반응에 미치는 효과

  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 조은지 (공주대학교 식품공학과) ;
  • 김이은 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.05.16
  • Accepted : 2018.07.11
  • Published : 2018.08.31

Abstract

Dendritic cells (DCs) are potent antigen-presenting cells that play a pivotal role in modulating both innate and adaptive immunity. This study examined the immunomodulatory activities of hot-water extracts of bee pollen (BPW) in bone-marrow derived DCs (BMDC) and mice splenocytes. BMDCs isolated from mice were treated with 250 and $500{\mu}g/mL$ BPW for 24 h. BPW, up to $500{\mu}g/mL$, did not display any cellular toxicity against BMDCs. In fact, it functionally induced BMDC activation via augmentation of CD80, CD86, and major histocompatibility complex (MHC) class I/II expression and pro-inflammatory cytokine (tumor necrosis factor; $TNF-{\alpha}$, interleukin; IL-6, and $IL-1{\beta}$) production. Interestingly, BPW treatment significantly increased the production of interferon $(IFN)-{\gamma}$ in splenocytes, suggesting its possible contribution to Th1 polarization in immune response. Taken together, these findings suggest that BPW may regulate innate and adaptive immunity via DC activation and Th1 polarization in immune responses.

Keywords

bee pollen;dendritic cells;splenocyte;immunomodulatory activity;cytokine production

References

  1. Andrade P, Ferreres F, Gil MI, Toms-Barbern FA. Determination of phenolic compounds in honeys with different floral origin by capillary zone electrophoresis. Food Chem. 60: 79-84 (1997) https://doi.org/10.1016/S0308-8146(96)00313-5
  2. Banchereau J. Steinman RM. Dendritic cells and the control of immunity. Nature 392: 245-252 (1998) https://doi.org/10.1038/32588
  3. Byun EH. Comparison study of immunomodulatory activity of polysaccharide and ethanol extracted from Sargassum fulvellum. J. Korean Soc. Food Sci. Nutr. 44: 1621-1628 (2015) https://doi.org/10.3746/jkfn.2015.44.11.1621
  4. Calixto JB, Campos MM, Otuki MF, Santos AR. Anti-inflammatory compounds of plant origin. Part II. modulation of proinflammatory cytokines, chemokines and adhesion molecules. Planta Med. 70: 93-103 (2004) https://doi.org/10.1055/s-2004-815483
  5. Cannon JG. Inflammatory cytokines in nonpathological states. News Physiol Sci. 15: 298-303 (2000)
  6. Chen FY, Ye YP, Sun HX, Li HX, Shi H. Stemucronatoside L, a pregnane glycoside from the roots of Stephanotis mucronata, inhibits Th1/Th2 immune responses in vitro. Chem. Biodivers. 6: 916-923 (2009) https://doi.org/10.1002/cbdv.200800159
  7. Cho EJ, Lee JH, Sung NY, Byun EH. Anti-inflammatory effect of Annona muricata Leaves Ethanol extracts. J. Kor. Soc. Food Sci. Nutr. 46: 681-687 (2017)
  8. Choi JH, Yim GY, Jang SY, Jeong YJ. Inhibition effect of the harmful food-born microorganisms on germination condition of acorn pollen. Korean J. Food Preserv. 14: 87-93 (2007)
  9. Chung YG, Yoon SH, Kwon JS, Bae MJ. Nutritional and biochemical studies on the pollen loads studies on lipid compositions of sunflower pollen load and effects of its pollen load on liver cholesterol metabolism in mouse. J. Kor. Soc. Food Nutr. 13: 169-174 (1984)
  10. Clerici M, Shearer GM. The Th1-Th2 hypothesis of HIV infection: new insights. Immunol. Today 15: 575-581 (1994) https://doi.org/10.1016/0167-5699(94)90220-8
  11. Gao X, Bjork L, Trajkovski V, Uggla M. Evaluation of antioxidant activities of rosehip ethanol extracts in different test systems. J. Sci. Food Agr. 80: 2021-2027 (2000) https://doi.org/10.1002/1097-0010(200011)80:14<2021::AID-JSFA745>3.0.CO;2-2
  12. Jin TY, Saravanakumar K, Wang MH. In vitro and in vivo antioxidant properties of water and methanol extracts of linden bee pollen. Biocatal. Agric. Biotechnol. 13: 186-189 (2018) https://doi.org/10.1016/j.bcab.2017.12.010
  13. Kim MJ, Bae NY, Kim KBWR, Park JH, Park SH, Cho YJ, Ahn DH. The anti-inflammatory effect of skipjack tuna (Katsuwonus pelamis) oil in LPS-induced RAW 264.7 cells and mouse models. Korean J. Biotechnol. Bioeng. 30: 326-331 (2015a)
  14. Kim SB, Jo YH, Liu Q, Ahn JH, Hong IP, Han SM, Hwang BY, Lee MK. Optimization of extraction condition of bee pollen using response surface amethodology: Correlation between anti-melanogenesis, antioxidant activity, and phenolic content. Molecules. 20: 19764-19774 (2015b)
  15. Kim JY, Jung KS, Jeong HG. Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS. Lett. 569: 321-326 (2004) https://doi.org/10.1016/j.febslet.2004.05.070
  16. Lee HJ, Lee CW, Choi MS, Son DJ, Hong JT. Effects of esthetic essential oils on LPS-induced nitric oxide generation in murine macrophage RAW 264.7 cells. J. Soc. Cosmet. Sci. Korea. 32: 111-116 (2006)
  17. Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK. Acemannan purified from Aloevera induces phenotypic and funtional maturation of immature dendritic cells. Int Immunopharmacol. 1: 1275-1284 (2001) https://doi.org/10.1016/S1567-5769(01)00052-2
  18. Lee TS, Shim SM, Im KH, Kim JW, Lee UY, Shim MJ, Lee MW. Studies on immuno-modulatory and antitumor effects crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31: 155-160 (2003) https://doi.org/10.4489/KJM.2003.31.3.155
  19. Lee K, Sohn Y, Lee MJ, Cho HS, Jang MH, Han NY, Shin KW, Kim SH, Cho IH, Bu Y, Jung HS. Effects of Angelica acutiloba on mast cell-mediated allergic reactions in vitro and in vivo. Immunopharm. Immunot. 34: 571-577 (2012) https://doi.org/10.3109/08923973.2011.636048
  20. Li F, Yuan QP, Rashid F. Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide from Crataegus pinnatifida Bge. Carbohydr Polym. 78: 80-88 (2009) https://doi.org/10.1016/j.carbpol.2009.04.005
  21. Maruyama H, Sakamoto T, Arki Y, Hara H. Anti-inflammatory effect of bee pollen ethanol extract from Citus sp. spanish on carrageenan-induced rat hind paw edema. BMC Complement Altern. Med. 10: 1472-1483 (2010)
  22. Medzhitov R. Toll-like receptors and immate immunity. Nat. Rev. Immunol 1: 135-145 (2001) https://doi.org/10.1038/35100529
  23. Mo ZQ, Wang JL, Yang M, Ni LY, Wang HQ, Lao GF, Li YW, Li AX, Luo XC, Dan XM. Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 postCryptocaryon irritans infection. Fish Shellfish Immun. 67: 467-474 (2017) https://doi.org/10.1016/j.fsi.2017.05.064
  24. Piani A, Hossle JP, Birchler T, Siegrist CA, Heumann D, Davies G, Loeliger S, Seger R, Lauener RP. Expression of MHC class II molecules contributes to lipopolysaccharide responsiveness. Eur. J. Immunol. 30: 3140-3146 (2000) https://doi.org/10.1002/1521-4141(200011)30:11<3140::AID-IMMU3140>3.0.CO;2-O
  25. Ryu HS, Kim J, Kim HS. Enhancing effect of Sorghum bicolor L. Moench (sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean J. Food Nutr. 19: 176-182 (2006)
  26. Ryu JB. Classification of honey plants in Korea. Korean J. Apiculture. 18: 5-22 (2003)
  27. Shan BE, Yoshida Y, Kuroda E, Yamashita U. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int. J. Immunopharmacol. 21: 59-70 (1999) https://doi.org/10.1016/S0192-0561(98)00063-0
  28. Shin SH, Kim DS, Kim SH, Jo SK, Byun MW, Yee ST. Effect of herbal composition (HemoHIM) on the activation of dendritic cells. J. Korean Soc Food Sci Nutr. 35: 1322-1328 (2006) https://doi.org/10.3746/jkfn.2006.35.10.1322
  29. Todd FE, Bretherick O. The composition of pollens. J. Econ. Entomol. 35: 312-317 (1942) https://doi.org/10.1093/jee/35.3.312
  30. Wink DA, Hines HB, Cheng RY, Switzer CH, Flores-Santana W, Vitek MP, Ridnour LA, Colton CA. Nitric oxide and redox mechanisms in the immune response. J. Leukoc Biol. 89: 873-891 (2011) https://doi.org/10.1189/jlb.1010550