DOI QR코드

DOI QR Code

Immunomodulatory activities of polysaccharides extracted from Cudrania tricuspidata fruits in macrophage

꾸지뽕(Cudrania tricuspidata) 열매에서 분리된 조다당의 큰포식세포 면역 활성 조절

  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 조은지 (공주대학교 식품공학과) ;
  • 김이은 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.07.04
  • Accepted : 2018.08.21
  • Published : 2018.10.31

Abstract

Macrophages play a crucial role in the host immune defense system. The current study investigated immunomodulatory activities induced by polysaccharides extracted from Cudrania tricuspidata (CTPS) fruits in murine macrophages and their role in signaling pathways. In macrophages, CTPS predominantly induced nitric oxide (NO), tumor necrosis factor-a, and interleukin-6 production. In addition, CTPS significantly up-regulated expression of the macrophage surface marker (CD80/86 and MHC class I/II). These results indicate that polysaccharides extracted from CTPS may potentially play an immunomodulatory role in macrophages via mitogen-activated protein kinases and nuclear factor-B signaling. These findings may be useful in the development of immune enhancing adjuvant materials obtained from natural sources.

Keywords

Cudrania tricuspidata fruits polysaccharide;immunomodulatory activity;macrophage;nitric oxide;cytokine

References

  1. Sung NY, Park YY, Kim YE, Cho EJ, Kim MH, Ryu GH, Byun EH, Park YJ. Immuno-modulatory activiies of polysaccharides separated from Chrysanthenuon zawadskii var. latilobum in macrophage cells. Korean J. Food Nutr. 49: 431-437 (2016)
  2. Youn KS, Kim JW. Antioxidant and angiotensin converting enzyme I inhibitory activities of extracts from Mulberry (Cudrania tricuspidata) fruit subjected to different drying methods. J. Korean Soc. Food Sci. Nutr. 41: 1388-1394 (2012)
  3. Yu AR, Park HY, Kim YS, Ha SK, Hong HD, Choi HD. Immuno-enhancing effect of seed extracts on a RAW 264.7 macrophage cell line. J. Korean Soc. Food Sci. Nutr. 41: 1671-1676 (2012)
  4. Kim J, Ryu HS, Shin JH, Kim HS. In vitro and ex vivo supplementation of Houttuynia cordata extract and immunomodulating effect in mice. J. Korean Soc. Food Sci. Nutr. 34: 167-175 (2005)
  5. Lee IK, Kim CJ, Song KS, Kim HM, Koshino H, Uramoto M, Yoo ID. Cytotoxic benzyl dihydroflavonols from Cudrania tricuspidata. Phytochemistry 41: 213-216 (1996)
  6. Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK. Acemannan purified from Aloevera induces phenotypic and functional maturation of immature dendritic cells. Int. Immunopharmacol. 1: 1275-1284 (2001)
  7. Mo ZQ, Wang JL, Yang M, Ni LY, Wang HQ, Lao GF, Li YW, Li AX, Luo XC, Dan XM. Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 post Cryptocaryon irritans infection. Fish Shellfish Immun. 67: 467-474 (2017)
  8. Ottersen T, Vance B, Doorenbos NJ, Chang BL, el-Feraly FS. The crystal structure of cudranone, 2,6,3'-trihydroxy-4-methoxy-2'-(3-methyl-2-butenyl)-benzophenone: a new antimicrobial agent from Cudrania chochinchinensis.Acta. Chem. Scand. B 31: 434-436 (1977)
  9. Piani A, Hossle JP, Birchler T, Siegrist CA, Heumann D, Davies G, Loeliger S, Seger R, Lauener RP. Expression of MHC class II molecules contributes to lipopolysaccharide responsiveness. Eur. J. Immunol. 30: 3140-3146 (2000)
  10. Schepetkin IA, Quinn MT. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int. Immunopharmacol. 6: 317-333 (2006)
  11. Seo MJ, Kang BW, Park JU, Kim MJ, Lee HH, Kim NH, Kim KH, Rhu EJ, Jeong YK. Effect of fermented Cudrania tricuspidata fruit extracts on the generation of the cytokines in mouse spleen cells. J. Life Sci. 23: 682-688 (2013)
  12. Cha JY, Kim HJ, Jun BS, Cho YS. Effects of water-extract of leaves from Morus alba and Cudrania tricuspi data on the lipid concentration of serum and liver in rats. J. Korean Soc. Agric. Chem. Biotechnol. 43: 303-308 (2000)
  13. Chen F, Nakashima N, Kimura I, Kimura M. Hypoglycemic activity and mechanisms of extracts from mulberry leaves (Folium mori) and cortex mori radicis in streptozotocin-induced diabetic mice. Yakugaku Zasshi 115: 476-482 (1995)
  14. Choi, SR, You, DH, Jang I, Ahn MS, Song EJ, Seo, S. Y, Choi MK, Kim YS, Kim MK. Choi DG. Cytotoxicity of methanol extracts from Cudrania tricuspidata Bureau. Korean J. Medicinal Corp Sci. 20: 153-158 (2012)
  15. Cobb MH, Goldsmith EJ. Dimerization in MAP-kinase signaling. Trends Biochem. Sci. 25: 7-9 (2000)
  16. Hano Y, Matsumoto Y, Sun JY. Nomura T. Structures of three new isoprenylated xanthones, cudraxanthones E, F and G. Planta Med. 56: 478-481 (1990)
  17. Hegde NR, Chevalier MS, Johnson DC. Viral inhibition of MHC class II antigen presentation. Trends Immunol. 24: 278-285 (2003)
  18. Jang IM. Treatise on Asian Herbal Medicines. Natural Products Science Seoul National University Press, Seoul, Korea (2003)
  19. Kang DG, Hur TY, Lee GM, Oh HC, Kwon TO, Sohn EJ, Lee HS. Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sci. 70: 2599-2609 (2002)
  20. Khawla BJ, Fatma C, Sameh M, Oumema NE, Claire BH, Raoudha EG. Structural, functional, and antioxidant properties of water-soluble polysaccharides from potatoes peels. Food Chem. 205: 97-105 (2016)
  21. Kim JY, Jung KS, Jeong HG. Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett. 569: 321-326 (2004)
  22. Bios MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958)
  23. Brewer MS, Ikins WG, Harbers CAAZ. TBA values, sensory characteristics, and volatiles in ground fork during long-term frozen storage: Effects of packaging. J. Food Sci. 57: 558-563 (1992)
  24. Byun MW, Byun EH. Immunological synergistic effects of combined treatment with herbal preparation (HemoHIM) and red ginseng extracts. J. Korean Soc. Food Sci. Nutr. 44: 182-190 (2015)
  25. Byun EB, Jang BS, Sung NY, Byun EH. Immunomodulatory activity of crude polysaccharide separated from Cudrania tricuspidata leaf. J. Korean Soc. Food Sci. Nutr. 45: 1099-1106 (2016)