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Protective effect of ethyl acetate fraction from Actinidia arguta sprout against high glucose-induced in vitro neurotoxicity

포도당으로 유도된 in vitro 뇌신경세포 독성에 대한 다래 순 아세트산에틸 분획물의 보호 효과

  • Yoo, Seul Ki (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jin Yong (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Su Bin (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Han, Hye Ju (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Chul-Wo (Division of Special Forest Products, National Institute of Forest Science) ;
  • Lee, Uk (Division of Special Forest Products, National Institute of Forest Science) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 유슬기 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 강진용 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 박수빈 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 한혜주 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김철우 (국립산림과학원 산림소득자원연구과) ;
  • 이욱 (국립산림과학원 산림소득자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Received : 2018.07.11
  • Accepted : 2018.08.23
  • Published : 2018.10.31

Abstract

The current study investigated in vitro anti-diabetic and neuroprotective effects of the ethyl acetate fraction in Actinidia arguta sprouts (EFAS), on $H_2O_2$ and high glucose-induced cytotoxicity in human neuroblastoma MC-IXC cells. EFAS had high total phenolic and total flavonoid contents. An assessment of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of EFAS, as well as its potential for inhibiting malondialdehyde production, indicated that EFAS may possess significant antioxidant properties. EFAS exerted inhibitory effects on ${\alpha}-glucosidase$ via glycemic regulation which forms advanced glycation end products. In addition, EFAS exhibited significant acetylcholinesterase inhibitory effects. Moreover, EFAS displayed protective effects against $H_2O_2$ and high glucose-induced cell death, and inhibited the generation of reactive oxygen species in MC-IXC cells. Finally, the main physiological compound of EFAS was identified via high performance liquid chromatography as a rutin.

Acknowledgement

Supported by : 산림청

References

  1. Pashikanti S, de Alba DR, Boissonneault GA, Cervantes-Laurean D. Rutin metabolites: novel inhibitors of nonoxidative advanced glycation end products. Free Radical Bio. Med. 48: 656-663 (2010) https://doi.org/10.1016/j.freeradbiomed.2009.11.019
  2. Schliebs R, Arendt T. The cholinergic system in aging and neuronal degeneration. Behav. brain Res. 221: 555-563 (2011) https://doi.org/10.1016/j.bbr.2010.11.058
  3. Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem. 85: 611-616 (2004) https://doi.org/10.1016/j.foodchem.2003.07.003
  4. Slominski A, Tobin DJ, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol. Rev. 84: 1155-1228 (2004) https://doi.org/10.1152/physrev.00044.2003
  5. Son ES, Oh SS, Han DS, Lee JM. Contents of total flavonoid and biological activities of edible plants. J. Korean Soc. Food Cult. 16: 504-514 (2001)
  6. Wallace DC. Mitochondrial genetics: A paradigm for aging and degenerative disease? Science 256: 628-632 (1992) https://doi.org/10.1126/science.1533953
  7. Wang X, Wang W, Li L, Perry G, Lee HG, Zhu X. Oxidative stress and mitochondrial dysfunction in Alzheimer's disease. Biochim. Biophys. Acta-Mol. Basis Dis. 1842: 1240-1247 (2014) https://doi.org/10.1016/j.bbadis.2013.10.015
  8. Watkins PB, Zimmerman HJ, Knapp MJ, Gracon SI, Lewis KW. Hepatotoxic effects of tacrine administration in patients with Alzheimer's disease. JAMA-J. Am. Med. Assoc. 271: 992-998 (1994) https://doi.org/10.1001/jama.1994.03510370044030
  9. Zhang W, Xu YC, Guo FJ, Meng Y, Li ML. Anti-diabetic effects of cinnamaldehyde and berberine and their impacts on retinol-binding protein 4 expression in rats with type 2 diabetes mellitus. Chin. Med. J. 121: 2124-2128 (2008)
  10. Kim JH, Yang HK, Hong HJ, Kang WY, Kim DG, Kim SC, Song KJ, King D, Han CH, Lee YJ. Neuroprotective effects of Korean kiwifruit against t-BHP-induced cell damage in PC12 cells. Korean J. Plant Resour. 23: 165-171 (2010)
  11. Kwak CS, Lee JH. In vitro antioxidant and anti-inflammatory effects of ethanol extracts from sprout of evening primrose (Oenothera laciniata) and gooseberry (Actinidia arguta). J. Korean Soc. Food Sci. Nutr. 43: 207-215 (2014) https://doi.org/10.3746/jkfn.2014.43.2.207
  12. Lee JW, Park JH, Kim JS, Choi EY, Han SN, Seong ES, Yu CY, Kwon YS, Kim MJ. Isolation of flavonol glycoside related to antioxidant activity from Hippophae rhamnoides leaves. Korean J. Medicinal Crop Sci. 19: 251-256 (2011) https://doi.org/10.7783/KJMCS.2011.19.4.251
  13. Lee AY, Kang MJ, Choe E, Kim JI. Hypoglycemic and antioxidant effects of Daraesoon (Actinidia arguta shoot) in animal models of diabetes mellitus. Nutr. Res. Pract. 9: 262-267 (2015a) https://doi.org/10.4162/nrp.2015.9.3.262
  14. Lee I, Lee BH, Eom SH, Oh CS, Kang H, Cho YS, Kim DO. Antioxidant capacity and protective effects on neuronal PC-12 cells of domestic bred kiwifruit. Korean J. Hort. Sci. Technol. 33: 259-267 (2015b) https://doi.org/10.7235/hort.2015.14123
  15. Mayne ST. Antioxidant nutrients and chronic disease: use of biomarkers of exposure and oxidative stress status in epidemiologic research. J. Nutr. 133: 933-940 (2003) https://doi.org/10.1093/jn/133.3.933S
  16. McPherson JD, Shilton BH, Walton DJ. Role of fructose in glycation and cross-linking of proteins. Biochem. 27: 1901-1907 (1988) https://doi.org/10.1021/bi00406a016
  17. Mousavi SH, Tayarani NZ, Parsaee H. Protective effect of saffron extract and crocin on reactive oxygen species-mediated high glucose-induced toxicity in PC12 cells. Cell. Molecular Neurobiol. 30: 185-191 (2010) https://doi.org/10.1007/s10571-009-9441-z
  18. Oh HJ, Jeon SB, Kang HY, Yang YJ, Kim SC, Lim SB. Chemical composition and antioxidative activity of kiwifruit in different cultivars and maturity. J. Korean Soc. Food Sci. Nutr. 40: 343-349 (2011) https://doi.org/10.3746/jkfn.2011.40.3.343
  19. Cyboran S, Oszmianski J, Kleszczynska H. Modification of the properties of biological membrane and its protection against oxidation by Actinidia arguta leaf extract. Chem. Biol. Interact. 222: 50-59 (2014) https://doi.org/10.1016/j.cbi.2014.08.012
  20. Ellman GL, Courtney KD, Andres Jr V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-95 (1961) https://doi.org/10.1016/0006-2952(61)90145-9
  21. Halliwell B, Gytteridge JM, Cross CE. Free radicals, antioxidants, and human disease: where are we now? J. Lab. Clin. Med. 119: 598-620 (1992)
  22. Jin DE, Park SK, Park CH, Seung TW, Choi SG, Heo HJ. Nutritional components of Korean traditional actinidia (Actinidia arguta) sprout and in vitro antioxidant effect. Korean J. Food Sci. Technol. 47: 37-43 (2015) https://doi.org/10.9721/KJFST.2015.47.1.37
  23. Kandimalla R, Thirumala V, Reddy PH, Hemachandra. Is Alzheimer's disease a type 3 diabetes? A critical appraisal. Biochim. Biophys. Acta-Mol. Basis Dis. 1863: 1078-1089 (2017) https://doi.org/10.1016/j.bbadis.2016.08.018
  24. Kang YK, Lee EA, Park HR. Neuroprotective effect according to reactive oxygen species scavenging activity from extracts of Cudrania tricuspidata leaves. Korean J. Food Cook. Sci. 28: 821-828 (2012) https://doi.org/10.9724/kfcs.2012.28.6.821
  25. Kim GH, Choi MH. Antioxidant activity of flavonoids in plant origin food. Korean J. Postharvest Sci. Technol. 6: 121-135 (1999)
  26. Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 81: 321-326 (2003) https://doi.org/10.1016/S0308-8146(02)00423-5
  27. Kim DI, Lee SH, Hur EY, Cho SM, Park HJ. Screening of natural plant resources with acetylcholinesterase inhibition and antioxidant activity. J. Korean Soc. Food Sci. Nutr. 34: 427-432 (2005) https://doi.org/10.3746/jkfn.2005.34.3.427
  28. Ahmed N. Advanced glycation endproducts-role in pathology of diabetic complications. Diabetes Res. Clin. Pract. 67: 3-21 (2005) https://doi.org/10.1016/j.diabres.2004.09.004
  29. Ahn H, Chung L, Choe E. In vitro antioxidant activity and ${\alpha}$-glucosidase and pancreatic lipase inhibitory activities of several Korean sanchae. Korean J. Food Sci. Technol. 47: 164-169 (2015) https://doi.org/10.9721/KJFST.2015.47.2.164
  30. Almeidaa D, Pintoa D, Santosa J, Vinha AF, Palmeira J, Ferreira HN, Rodriguesa F, Beatriz M, Oliveira PP. Hardy kiwifruit leaves (Actinidia arguta): An extraordinary source of value-added compounds for food industry. Food Chem. 259: 113-121 (2018) https://doi.org/10.1016/j.foodchem.2018.03.113
  31. Apostolidis E, Kwon YI, Shetty K. Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innov. Food Sci. Emerg. Technol. 8: 46-54 (2007) https://doi.org/10.1016/j.ifset.2006.06.001
  32. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 414: 813-820 (2001) https://doi.org/10.1038/414813a
  33. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agr. Food Chem. 49: 3420-3424 (2001) https://doi.org/10.1021/jf0100907