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Antioxidant and neuroprotective effects of crude polysaccharide fractions from Cudrania tricuspidata fruits

꾸지뽕 열매 조다당류 분획물의 산화방지 활성 및 신경세포 보호 효과

  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 김이은 (공주대학교 식품공학과) ;
  • 조은지 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.07.30
  • Accepted : 2018.08.29
  • Published : 2018.10.31

Abstract

The current study examined antioxidant and neuronal cell protective effects of the crude polysaccharide fraction in Cudrania tricuspidata fruits (CTP). The radical scavenging activities of (1,1-diphenyl-picrylhydrazyl and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) and reducing power and FRAP of CTP were increased dose-dependently. In addition, the expression of neuroprotective effect of CTP was tested in HT22 mouse hippocampal cells. CTP treatment exhibited non cytotoxicity at dose levels below $500{\mu}g/mL$. Within this optimal concentration range, CTP treatment significantly increased cell viability in $H_2O_2-treated$ HT22 cells. Furthermore, CTP treatment increased superoxide dismutase (SOD) activity and decreased malonaldehyde (MDA) levels in HT22 cells. Therefore, these results indicate that the crude polysaccharide fraction from Cudrania tricuspidata fruits (CTP) possesses antioxidant activities and displays therapeutic potential as a useful source material in the development of brain disorder treatments targeting oxidative stress in neuronal cells.

Keywords

Cudrania tricuspidata fruits;polysaccharide;neuroprotective effects;antioxidant;neuronal cell

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