Comparative study of serological detection methods on old bloodstain samples

오래된 혈흔 시료에 대한 혈청학적 식별법의 비교 연구

  • Lee, Minji (Forensic DNA division, National Forensic Service) ;
  • Ahn, Eu-Ree (Forensic DNA division, National Forensic Service) ;
  • Kim, Da-Hye (Forensic DNA division, National Forensic Service) ;
  • Shin, Heejin (Forensic DNA division, National Forensic Service) ;
  • Jung, Ju Yeon (Forensic DNA division, National Forensic Service) ;
  • Lee, Sung-Jin (Forensic DNA division, National Forensic Service) ;
  • Chun, Byung-Won (Forensic DNA division, National Forensic Service)
  • 이민지 (국립과학수사연구원 법유전자과) ;
  • 안으리 (국립과학수사연구원 법유전자과) ;
  • 김다혜 (국립과학수사연구원 법유전자과) ;
  • 신희진 (국립과학수사연구원 법유전자과) ;
  • 정주연 (국립과학수사연구원 법유전자과) ;
  • 이성진 (국립과학수사연구원 법유전자과) ;
  • 전병원 (국립과학수사연구원 법유전자과)
  • Received : 2018.08.21
  • Accepted : 2018.10.06
  • Published : 2018.10.25


Blood is a commonly found body fluid at crime scenes, and plays an important role in identifying suspects and in the reconstruction of crime scenes. Although serological detection of blood has been widely used in the field of forensic science, research on the detection of old bloodstains is scarce. This work aimed to compare various methods for the detection of old bloodstains and validate the reliability of their results. Four presumptive tests-Tetramethylbenzidine, $Bluestar^{(R)}$, Leucomalachite Green, Kastle-Meyer tests-and two confirmatory tests-Fecal Occult Blood (FOB) and Rapid Stain $Identification^{(TM)}-Blood$ ($RSID^{TM}-Blood$) tests-were compared. Bloodstain samples from post-mortem cases were collected on gauzes and then stored at room temperature for periods from 7 to 30 years. All the presumptive tests were positive, even for the 30-year-old sample. However, FOB and $RSID^{TM}-Blood$ provided false negative results for some samples stored for 17 years or more (1988 to 2001). The results indicate that FOB and $RSID^{TM}-Blood$ are not reliable for the detection of old bloodstains. These findings can be useful in the selection of an appropriate detection method for serological testing of old bloodstains. In addition, the information will be useful background knowledge when applied in the field of forensic practice.


old bloodstain;serological detection;presumptive test;confirmatory test


Supported by : 국립과학수사연구원


  1. K. Virkler and I. K. Lednev, Forensic Sci. Int., 188, 1-17 (2009).
  2. H. Y. Lee, K.-J. Shin, and W. I. Yang, Korean J. Leg Med., 33, 84-88 (2009).
  3. J. H. An, K-J. Shin, and W. I. Yang. BMB Rep., 45(10), 545-553 (2012).
  4. L. Kobilinsky, 'Forensic Chemistry Handbook', John Wiley&Sons, New Jersey, 2011.
  5. 한면수, 과학수사와 증거재판에서 DNA프로필의 역할, 경찰학연구 제4호, 경찰대학, 173-269 (2003).
  6. R. Li, 'Forensic Biology', 2nd Ed., Boca Raton, 2008.
  7. J. Siegel and P. Saukko, 'Encyclopedia of Forensic Sciences', 2nd ed., London, 2012.
  8. R. Saferstein, 'Forensic Science Handbook', Vol. 2, New Jersey, 1988.
  9. L. J. Blum, P. Esperanca, and S. Rocquefelte., Can. Soc. Forensic Sci. J., 39. 81-100 (2006).
  10. J. L. Webb, J. I. Creamer, and T. I. Quichenden., Luminescence, 21(4), 214-220 (2006).
  11. M. vandewoestyne and T. lepez., J. Forensic Sci., 60(3), 707-711 (2015).
  12. S. S. Tobe, N. Watson, and N. N. Daeid, J. Forensic Sci., 52(1), 102-109 (2007).
  13. E. Johnston, C. E. Ames, K. E. Dagnall, and J. Foster., J. Forensic Sci., 53(3), 687-689 (2008).
  14. S. K. Lim, K. W. Park, and S. K. Choi., Anal. Sci. & Technol., 17, 211-216 (2004).
  15. I. horjan, L. Barbaric, and G. Mrsic., J. Forensic Leg Med., 38, 101-105 (2016).
  16. S. Turrina and G. Filippini., Forensic Sci. Int. Genet. Suppl. Series, 1, 74-75 (2008).
  17. H. C. Lee, T. Palmbach, and M. T. Miller. 'Henry Lee's Crime scene handbook. '1st Ed., London, 2001.
  18. 김택수, 황문규, 김슬기, '중요강력범죄 장기미제사건 수사를 위한 효율적 제도 연구', 경찰청 용역연구보고서, 2013.
  19. Claudia Gomes, Cesar Lopez-Matayoshi, Sara Palome-Diez, Ana Maria Lopez-Parra, Pedro Cuesta-Alvaro, Carlos Baeza-Richer, Juan F. Gibaja, and Eduardo Arroyo-Pardo, Forensic Sci. Int. Genet. Suppl. Series, 6, e546-e548 (2017).
  20. Blanchard, D., Transfus. Med. Rev., 4(3), 170-186 (1990).
  21. S. Alters, 'Biology: Understanding Life', 3rd Ed., Jones & Bartlett Learning, Massachusetts, (2000).