GABAergic neuronal development in the embryonic mesencephalon of mice

  • Kim, Mun-Ki (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Si-Joon (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Vasudevan, Anju (Angiogenesis and Brain Development Laboratory, McLean Hospital/Harvard Medical School) ;
  • Won, Chung-Kil (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University)
  • Received : 2019.10.30
  • Accepted : 2019.11.15
  • Published : 2019.12.31


This study presents neurogenesis and neuronal migration patterns of gamma-aminobutyric acid-ergic (GABAergic) neurons during mesencephalic development of mouse. After neurons from embryonic day (E) 10-16 were labelled by a single injection of 5-bromo-2'-deoxyuridine (BrdU), immunohistochemistry was performed. Neurogenesis were mainly generated in the mesencephalic region at E10 to E13. After E14, BrdU positive cells were observed only in the dorsal mesencephalon. GABAergic neurons were mainly originated in the ventrolateral region of the mesencephalon at the early embryonic stage, especially at E11 to E13. E10-labeled cells showed positive for GABAergic neuron in the basal plate of the mesencephalon at E13. At E15, GABAergic neurons were observed in the entire basal plate and some regions of the ventral and dorsal mesencephalon. They were present in the whole basal plate, the ventral and dorsal mesencephalon of E17, spreading more outward of the mesencephalon at P0. Our study demonstrates that major neurogenesis of GABAergic neurons occurs at E11 to E13. However, neuronal migration continues until neonatal period during mesencephalic development.


Supported by : National Research Foundation of Korea (NRF)


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