- Volume 59 Issue 4
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GABAergic neuronal development in the embryonic mesencephalon of mice
- Kim, Mun-Ki (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
- Lee, Si-Joon (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
- Vasudevan, Anju (Angiogenesis and Brain Development Laboratory, McLean Hospital/Harvard Medical School) ;
- Won, Chung-Kil (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University)
- Received : 2019.10.30
- Accepted : 2019.11.15
- Published : 2019.12.31
This study presents neurogenesis and neuronal migration patterns of gamma-aminobutyric acid-ergic (GABAergic) neurons during mesencephalic development of mouse. After neurons from embryonic day (E) 10-16 were labelled by a single injection of 5-bromo-2'-deoxyuridine (BrdU), immunohistochemistry was performed. Neurogenesis were mainly generated in the mesencephalic region at E10 to E13. After E14, BrdU positive cells were observed only in the dorsal mesencephalon. GABAergic neurons were mainly originated in the ventrolateral region of the mesencephalon at the early embryonic stage, especially at E11 to E13. E10-labeled cells showed positive for GABAergic neuron in the basal plate of the mesencephalon at E13. At E15, GABAergic neurons were observed in the entire basal plate and some regions of the ventral and dorsal mesencephalon. They were present in the whole basal plate, the ventral and dorsal mesencephalon of E17, spreading more outward of the mesencephalon at P0. Our study demonstrates that major neurogenesis of GABAergic neurons occurs at E11 to E13. However, neuronal migration continues until neonatal period during mesencephalic development.
Supported by : National Research Foundation of Korea (NRF)
- Hatten ME. New directions in neuronal migration. Science 2002;297:1660-1663. https://doi.org/10.1126/science.1074572
- Li S, Joshee S, Vasudevan A. Mesencephalic GABA neuronal development: no more on the other side of oblivion. Biomol Concepts 2014;5:371-382.
- Vasudevan A, Won C, Li S, Erdelyi F, Szabo G, Kim KS. Dopaminergic neurons modulate GABA neuron migration in the embryonic midbrain. Development 2012;139:3136-3141. https://doi.org/10.1242/dev.078394
- Altman J, Bayer SA. Development of the brain stem in the rat. V. Thymidine-radiographic study of the time of origin of neurons in the midbrain tegmentum. J Comp Neurol 1981;198:677-716. https://doi.org/10.1002/cne.901980409
- Barbaresi P. Postnatal development of GABA-immunoreactive neurons and terminals in rat periaqueductal gray matter: a light and electron microscopic study. J Comp Neurol 2010;518:2240-2260. https://doi.org/10.1002/cne.22329
- Fahn S, Cote LJ. Regional distribution of gamma-aminobutyric acid (GABA) in brain of the rhesus monkey. J Neurochem 1968;15:209-213. https://doi.org/10.1111/j.1471-4159.1968.tb06198.x
- Mize RR. The organization of GABAergic neurons in the mammalian superior colliculus. Prog Brain Res 1992;90:219-248.
- Benes FM, Berretta S. GABAergic interneurons: implications for understanding schizophrenia and bipolar disorder. Neuropsychopharmacology 2001;25:1-27. https://doi.org/10.1016/S0893-133X(01)00225-1
- Rakic P. Mode of cell migration to the superficial layers of fetal monkey neocortex. J Comp Neurol 1972;145:61-83. https://doi.org/10.1002/cne.901450105
- Anderson SA, Eisenstat DD, Shi L, Rubenstein JL. Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes. Science 1997;278:474-476. https://doi.org/10.1126/science.278.5337.474
- Letinic K, Zoncu R, Rakic P. Origin of GABAergic neurons in the human neocortex. Nature 2002;417:645-649. https://doi.org/10.1038/nature00779
- Tsunekawa N, Yanagawa Y, Obata K. Development of GABAergic neurons from the ventricular zone in the superior colliculus of the mouse. Neurosci Res 2005;51:243-251. https://doi.org/10.1016/j.neures.2004.11.011
- Corbin JG, Nery S, Fishell G. Telencephalic cells take a tangent: non-radial migration in the mammalian forebrain. Nat Neurosci 2001;4 Suppl:1177-1182. https://doi.org/10.1038/nn749
- Marin O, Rubenstein JL. A long, remarkable journey: tangential migration in the telencephalon. Nat Rev Neurosci 2001;2:780-790.
- Parnavelas JG. The origin and migration of cortical neurones: new vistas. Trends Neurosci 2000;23:126-131. https://doi.org/10.1016/S0166-2236(00)01553-8
- Nadarajah B, Parnavelas JG. Modes of neuronal migration in the developing cerebral cortex. Nat Rev Neurosci 2002;3:423-432. https://doi.org/10.1038/nrn845
- Lewandowski TA, Ponce RA, Charleston JS, Hong S, Faustman EM. Changes in cell cycle parameters and cell number in the rat midbrain during organogenesis. Brain Res Dev Brain Res 2003;141:117-128. https://doi.org/10.1016/S0165-3806(03)00004-X
- Soriano E, Del Rio JA. Simultaneous immunocytochemical visualization of bromodeoxyuridine and neural tissue antigens. J Histochem Cytochem 1991;39:255-263. https://doi.org/10.1177/39.3.1671576
- Lahti L, Achim K, Partanen J. Molecular regulation of GABAergic neuron differentiation and diversity in the developing midbrain. Acta Physiol (Oxf) 2013;207:616-627. https://doi.org/10.1111/apha.12062
- Bayer SA, Wills KV, Triarhou LC, Ghetti B. Time of neuron origin and gradients of neurogenesis in midbrain dopaminergic neurons in the mouse. Exp Brain Res 1995;105:191-199.
- Kim MK, Lee SJ, Vasudevan A, Won C. Neurogenesis and neuronal migration of dopaminergic neurons during mesencephalon development in mice. J Biomed Transl Res 2018;19:125-129. https://doi.org/10.12729/jbtr.2018.19.4.125
- Nair-Roberts RG, Chatelain-Badie SD, Benson E, White-Cooper H, Bolam JP, Ungless MA. Stereological estimates of dopaminergic, GABAergic and glutamatergic neurons in the ventral tegmental area, substantia nigra and retrorubral field in the rat. Neuroscience 2008;152:1024-1031. https://doi.org/10.1016/j.neuroscience.2008.01.046
- Korotkova TM, Ponomarenko AA, Brown RE, Haas HL. Functional diversity of ventral midbrain dopamine and GABAergic neurons. Mol Neurobiol 2004;29:243-259. https://doi.org/10.1385/MN:29:3:243