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Skin lightening effect of fermented Panax ginseng extract

자연삼 발효 추출물의 미백 활성에 대한 연구

Lee, Hyosung
이효성

  • Received : 2018.12.21
  • Accepted : 2019.02.20
  • Published : 2019.02.28

Abstract

Panax ginseng is known for various pharmacological activities mainly due to saponins. Since minor saponins, generated by the decomposition of major saponins, generally exert higher activities than major saponins, the fermentation may increase the minor saponin contents in ginseng products. In this study, we tested fermented ginseng extract whether or not provide a safe cosmetic ingredient for whitening purpose. In this regard, fermented Ginseng extract was prepared and evaluated the inhibitory activity toward tyrosinase and the melanin synthesis suppression. The safety was tested via cell viability and toxicity test. The skin lightening effect was also evaluated by clinical study. The fermented Ginseng extract exerted higher activities in tyrosine inhibition and in suppressing melanin synthesis compared to Kojic acid and arbutin. In the clinical test, skin lightening effecte of the sample was clearly higher than vehicle or Vitamin C. We thus concluded that the fermented Ginseng extract may provide a safe cosmetic ingredient for skin lightening purpose.

Keywords

fermented ginseng extract;Skin lightening;melanin synthesis;pigmentation

References

  1. S. Del Bino, C. Duval, F. Bernerd. (2018). Clinical and biological characterization of skin pigmentation diversity and its consequences on UV impact International journal of Molecular Sciences, 19(9), 2668-2712. https://doi.org/10.3390/ijms19092668
  2. T. Pillaiyar, M. Manickam, V. Namasivayam. (2017). Skin whitening agents: medicinal chemistry perspective of tyrosinase inhibitors. Journal of Enzyme inhibition and Medicinal Chemistry, 32(1), 403-425. https://doi.org/10.1080/14756366.2016.1256882
  3. B. Desmedt et al. (2016). In vitro Dermal Absorption of Hydroquinone: Protocol Validation and applicability on illegal skin-whitening cosmetics. Skin Pharmacoogy and Physiology, 29(6), 300-308. https://doi.org/10.1159/000454719
  4. S. Sonthalia, D. Daulatabad, R. Sarkar. (2016). Glutathione as a skin whitening agent: Facts, myths, evidence and controversies. Indian journal of dermatology, venereology and leprology, 82(2), 262-72.
  5. J. Kim. (2018). Pharmacological and medical applications of Panax ginseng and ginsenosides: a review for use in cardiovascular diseases. Journal of Ginseng Reseach, 42(3), 264-269. https://doi.org/10.1016/j.jgr.2017.10.004
  6. D. Tam et al. (2018). Ginsenoside Rh1: A systematic review of its pharmacological properties. Planta Medica, 84(3), 139-152. https://doi.org/10.1055/s-0043-124087
  7. S. J. Lee, N. Ha, Y. Kim, M. G. Kim. (2016). Changes in the ginsenoside content during fermentation using an appliance for the preparation of red ginseng. American Journal of Chinese Medicine, 44(8), 1595-1606. https://doi.org/10.1142/S0192415X16500890
  8. E. Kim. (2006). A Study of Whitening Cosmetics from natural products. Korean Journal of Aesthetics and Cosmetics Society, 4(2), 195-203.
  9. S. W. Hwang et al. (2018). Fermentation-mediated enhancement of ginseng’s anti-allergic activity against IgE-mediated passive cutaneous anaphylaxis in vivo and in vitro. Journal of Microbiology and Biotechnology, 28(10), 1626-1634. https://doi.org/10.4014/jmb.1807.07057
  10. A. Di Petrillo et al. (2016). Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts. BMC Complementary and Alternative Medicine, 16(1), 453-461. https://doi.org/10.1186/s12906-016-1442-0
  11. H. B. Kim, K. C. Park, J. H. Park, C. G. Lee, S. K. Ye, J. Y. Park. (2016). Inhibition of tyrosinase activity and melanin production by the chalcone derivative 1-(2-cyclohexylmethoxy-6-hydroxy-phenyl)-3-(4-hydroxymethyl-phenyl)-propenone. Biochemical and Biophysical Research Communications, 480(4), 648-654. https://doi.org/10.1016/j.bbrc.2016.10.110
  12. F. Angius, A. Floris. (2015). Liposomes and MTT cell viability assay: an incompatible affair. Toxicology In Vitro, 29(2), 314-319. https://doi.org/10.1016/j.tiv.2014.11.009
  13. G. Fotakis, J. A. Timbrell. (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology letter, 160(2), 171-177. https://doi.org/10.1016/j.toxlet.2005.07.001
  14. Y. Tian, T. Hoshino, C. J. Chen, Y. E, S. Yabe, W. Liu. (2009). The evaluation of whitening efficacy of cosmetic products using a human skin pigmentation spot model. Skin Research and Technology, 15(2), 218-223. https://doi.org/10.1111/j.1600-0846.2009.00358.x
  15. E. V. Curto et al. (1999). Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochemical Pharmacology, 57(6), 663-672. https://doi.org/10.1016/S0006-2952(98)00340-2