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Setting Behavior and Drug Release from Brushite Bone Cement prepared with Granulated Hydroxyapatite and β-Tricalcium Phosphate

  • Son, Yeong-Jun (School of Advanced Materials Engineering, The Center of Biomedical Materials and Biotechnology, Andong National University) ;
  • Lee, In-Cheol (School of Advanced Materials Engineering, The Center of Biomedical Materials and Biotechnology, Andong National University) ;
  • Jo, Hyun-Ho (School of Advanced Materials Engineering, The Center of Biomedical Materials and Biotechnology, Andong National University) ;
  • Chung, Tai-Joo (School of Advanced Materials Engineering, The Center of Biomedical Materials and Biotechnology, Andong National University) ;
  • Oh, Kyung-Sik (School of Advanced Materials Engineering, The Center of Biomedical Materials and Biotechnology, Andong National University)
  • Received : 2018.10.03
  • Accepted : 2018.12.10
  • Published : 2019.01.31

Abstract

Calcium phosphate bone cement was prepared to contain antibiotics for release after setting using granulated ${\beta}$-tricalcium phosphate (${\beta}$-TCP) and hydroxyapatite (HA). Gentamicin sulfate (GS) solution was infiltrated within the interconnected pores of the granule to avoid affecting the setting reaction and for protection of GS during the setting. Consequently, the setting time and the temperature increase were not affected, regardless of the loading of GS. The release of the GS from the cement was estimated by measuring the concentration at regular intervals from the cement dipped solution. The ${\beta}$-TCP granule loaded with GS exhibited the saturation of accumulative concentration at 16 h. In contrast, the HA granule with GS exhibited steady increase in accumulative concentration of over $10{\mu}g/ml$ at 144 h. Thus, the granulated cement could release the GS greater than the minimum inhibitory concentration of staphylococcus during the prescription peroid of the oral antibiotics.

Keywords

Bone cement;Granule;Gentamicin;Hydroxyapatite;${\beta}$-TCP

Acknowledgement

Supported by : Andong National University

References

  1. M. Bohner, "Calcium Orthophosphates in Medicine: from Ceramics to Calcium Phosphate Cements," Injury, 31 [S4] D37-47 (2000). https://doi.org/10.1016/S0020-1383(00)80022-4
  2. M. Bohner, J. Lemaitre, P. Van Landuyt, P. Y. Zambelli, H. P. Merkle, and B. Gander, "Gentamicin-Loaded Hydraulic Calcium Phosphate Bone Cement as Antibiotic Delivery System," J. Pharm. Sci., 86 [5] 565-72 (1997). https://doi.org/10.1021/js960405a
  3. K. Hartke, H. Hartke, E. Mutschler, G. Rucker, and M. Wichtl, "Gentamicinsulfat (Kommentar)," pp. G11-1-G11-6 in Deutsches Arzneibuch 10 Kommentar fur Studierende, Govi-Verlag, Fankfort, 1991.
  4. J. Randzio, K. Thoma, R. Alex, and B. Rhomberg, "Healing and Pharmacokinetics of a Beta-Tricalcium Phosphate-Gentamycin Combination in Animal Studies (Preliminary Report)," Dtsch. Zahnarztl. Z., 40 [6] 668-71 (1985).
  5. F. Theiss, D. Apelt, B. Brand, A. Kutter, K. Zlinksky, M. Bohner, S. Matter, C. Frei, J. A. Auer, and B. von Rechenber, "Biocompatibility and Resorption of a Brushite Calcium Phosphate Cement," Biomaterials, 26 [21] 4383-94 (2005). https://doi.org/10.1016/j.biomaterials.2004.11.056
  6. Walenkamp GHIM, Gentamicin-PMMA Beads; pp. 50-7, Ed. by Merck, Merck, Darmstadt, 1983.
  7. M. Bohner, J. Lemaitre, H. P. Merkle, and B. Gander, "Control of Gentamicin Release from a Calcium Phosphate Cement by Admixed Poly(Acrylic Acid)," J. Pharm. Sci., 89 [10] 1262-70 (2000). https://doi.org/10.1002/1520-6017(200010)89:10<1262::AID-JPS4>3.0.CO;2-7
  8. K. S. Oh, H. W. Choi, and S. R. Kim, "Temperature Rise and Setting of ${\beta}$-TCP-MCPM Bone Cement Containing Dense ${\beta}$-TCP Granules," Curr. Appl. Phys., 5 [5] 489-92 (2005). https://doi.org/10.1016/j.cap.2005.01.016
  9. A. A. Mirtchi, J. Lemaitre, and N. Terao, "Calcium Phosphate Cements: Study of the ${\beta}$-Tricalicum Phosphate-Monocalcium Phosphate System," Biomaterials, 10 [7] 475-80 (1989). https://doi.org/10.1016/0142-9612(89)90089-6
  10. K. Ohura, M. Bohner, P. Hardouin, J. Lemaitre, G. Pasquier, and B. Flautre, "Resorption of, and Bone Formation from, New ${\beta}$-Tricalcium Phosphate-Monocalcium Phosphate Cements: An in vivo Study," J. Biomed. Mater. Res. 30 [2] 193-200 (1996). https://doi.org/10.1002/(SICI)1097-4636(199602)30:2<193::AID-JBM9>3.0.CO;2-M
  11. K. S. Oh, S. R. Kim, and P. Boch, "Synthesis and Properties of Bone Cement Containing Dense ${\beta}$-TCP Granules," Key Eng. Mater. 254-256 237-40 (2004).
  12. K. S. Oh, and S. R. Kim, "Effect of the Composition in ${\beta}$-TCP MCPM Bone Cement Containing Dense ${\beta}$-TCP Granules," Key Eng. Mater., 284-286 141-44 (2005). https://doi.org/10.4028/www.scientific.net/KEM.284-286.141
  13. S. A. Lee, T. J. Chung, and K. S. Oh, "Effect of Storage Conditions on the Setting Properties of Brushite Bone Cement Containing Granular ${\beta}$-Tricalcium Phosphate," J. Korean Ceram. Soc., 45 [10] 624-29 (2008).
  14. S. V. Dorozhkin, Calcium Orthophosphates: Applications in Nature, Biology, and Medicine; p. 850, Pan Stanford, Singapore, 2012.
  15. S. M. Seo, D. M. Kim, T. J. Chung, J. J. Yoo, H. J. Kim, H. J. Chun, J. W. Jang, and K. S. Oh, "Effect of Milling time on the Viscosity of Hydroxyapatite Suspension," Curr. Appl. Phys. 12 [S2] S71-5 (2012).
  16. G. Bertrand, P. Roy, C. Filiatre, and C. Coddet, "Spray-Dried Ceramic Powders: A Quantitative Correlation between Slurry Characteristics and Shapes of the Granules," Chem. Eng. Sci., 60 [1] 95-102 (2005). https://doi.org/10.1016/j.ces.2004.04.042
  17. B. E. Rosenkrantz, J. R. Greco, J. G. Hoogerheide, and E. M. Oden, "Gentamicin Sulfate," pp. 295-340 in Analytical Profiles of Drug Substances, Vol 9, Ed. by K. Florey, Academic Press, New York, 1981.
  18. J. Morant and H. Ruppaner, Arzneimittelkompendium der Schweiz; p. 834, Basler Zeitung, Basel, 1996.
  19. U. Gbureck, S. Dembski, R. Thull, and J. E. Barralet, "Factors Influencing Calcium Phosphate Cement Shelf-Life," Biomaterials, 26 [17] 3691-97 (2005). https://doi.org/10.1016/j.biomaterials.2004.09.036