Inhibitory effects of crude polysaccharide fractions from Annona muricata L. on melanogenesis

그라비올라 잎(Annona muricata L.) 조다당 분획분의 멜라닌 생성 저해 효과

  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 김이은 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.10.17
  • Accepted : 2018.12.17
  • Published : 2019.02.28


The objective of this study was to evaluate the anti-melanogenic effects of crude polysaccharide fractions from Annona muricata L. (ALP) in 3-isobutyl-1-methylxanthine (IBMX) stimulating hormone-induced mouse B16F10 melanoma cells. The inhibitory effect of ALP on tyrosinase activity was approximately $33.88{\pm}0.79%$ at 5 mg/mL. Additionally, the B16F10 cellular tyrosinase and melanin synthesis inhibition activities by ALP were $54.21{\pm}4.76$ and $56.74{\pm}6.97%$ at $250{\mu}g/mL$, respectively. Similarly, whitening-related protein tyrosinase, tyrosinase-related protein 1 (TRP-1) and TRP-2, and microphthalmia-associated transcription factor (MITF) were reduced by ALP treatment. These results indicated that ALP could be used as a functional cosmetic ingredient after confirming its whitening activity related to melanin content.

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Fig. 2. Cell viability of B16F10 melanoma cells after treating crude polysaccharide fractions from Annona muricata L. (ALP) and IBMX.

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Fig. 3. The difference of melanin contents in B16F10 mouse melanoma cells treated with various solvent fractions of crude polysaccharide fractions from Annona muricata L. (ALP).

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Fig. 4. Effect of crude polysaccharide fractions from Annona muricata L. (ALP) on tyrosinase and melanin synthesis in B16F10 mouse melanoma cells treated with 3-isobutyl-1-methylxanthine (IBMX; 200 μM).

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Fig. 5. Effects crude polysaccharide fractions from Annona muricata L. (ALP) on the levels of protein expression of TRP-1, TRP-2, MITF, Tyrosine, p-CREB in B16F10 mouse melanoma cells.

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Fig. 1. Tyrosinase inhibition activity of crude polysaccharide fractions from Annona muricata L. (ALP).


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