DOI QR코드

DOI QR Code

Inhibitory effects of crude polysaccharide fractions from Annona muricata L. on melanogenesis

그라비올라 잎(Annona muricata L.) 조다당 분획분의 멜라닌 생성 저해 효과

  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 김이은 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.10.17
  • Accepted : 2018.12.17
  • Published : 2019.02.28

Abstract

The objective of this study was to evaluate the anti-melanogenic effects of crude polysaccharide fractions from Annona muricata L. (ALP) in 3-isobutyl-1-methylxanthine (IBMX) stimulating hormone-induced mouse B16F10 melanoma cells. The inhibitory effect of ALP on tyrosinase activity was approximately $33.88{\pm}0.79%$ at 5 mg/mL. Additionally, the B16F10 cellular tyrosinase and melanin synthesis inhibition activities by ALP were $54.21{\pm}4.76$ and $56.74{\pm}6.97%$ at $250{\mu}g/mL$, respectively. Similarly, whitening-related protein tyrosinase, tyrosinase-related protein 1 (TRP-1) and TRP-2, and microphthalmia-associated transcription factor (MITF) were reduced by ALP treatment. These results indicated that ALP could be used as a functional cosmetic ingredient after confirming its whitening activity related to melanin content.

Keywords

Annona muricata L.;tyrosinase;melanin synthesis;whitening activity;microphthalmia-associated transcription factor (MITF)

SPGHB5_2019_v51n1_52_f0001.png 이미지

Fig. 2. Cell viability of B16F10 melanoma cells after treating crude polysaccharide fractions from Annona muricata L. (ALP) and IBMX.

SPGHB5_2019_v51n1_52_f0003.png 이미지

Fig. 3. The difference of melanin contents in B16F10 mouse melanoma cells treated with various solvent fractions of crude polysaccharide fractions from Annona muricata L. (ALP).

SPGHB5_2019_v51n1_52_f0004.png 이미지

Fig. 4. Effect of crude polysaccharide fractions from Annona muricata L. (ALP) on tyrosinase and melanin synthesis in B16F10 mouse melanoma cells treated with 3-isobutyl-1-methylxanthine (IBMX; 200 μM).

SPGHB5_2019_v51n1_52_f0005.png 이미지

Fig. 5. Effects crude polysaccharide fractions from Annona muricata L. (ALP) on the levels of protein expression of TRP-1, TRP-2, MITF, Tyrosine, p-CREB in B16F10 mouse melanoma cells.

SPGHB5_2019_v51n1_52_f0006.png 이미지

Fig. 1. Tyrosinase inhibition activity of crude polysaccharide fractions from Annona muricata L. (ALP).

References

  1. Adewole SO, Ojewole JA. Protective effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocin-treated diabetic rats. Afr. J. Tradit. Complem. 6: 30-41 (2008)
  2. Ando H, Niki Y, Ito M, Akiyama K, Matsui MS, Yarosh DB, Ichihashi M. Melanosomes are transferred from melanocytes to feratinocytes through the processes of packaging, release, uptake and dispersion. J. Invest. Dermatol.. 132: 1222-1229 (2012) https://doi.org/10.1038/jid.2011.413
  3. Boguniewicz M, Leung YM. Atopic dermatitis: a disease of altered skin barrier and immune dysregulation. Immunology Review. 242: 233 (2011) https://doi.org/10.1111/j.1600-065X.2011.01027.x
  4. Berneburg M, Plettengerg H, Krutmann J. Photoaging of human skin, Photodermatol. Photoimmunol. Photomed. 16: 239 (2000) https://doi.org/10.1034/j.1600-0781.2000.160601.x
  5. Carbajal D, Casaco A, Arruzazabala L, Gonzalez R, Fuentes V. Pharmacological screening of plant decoctions commonly used in Cuban folk medicine. J. Ethnopharmacol. 33: 21-24 (1991) https://doi.org/10.1016/0378-8741(91)90155-7
  6. Chan YY, Kim KH, Cheah SH. Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. J. Ethnopharmacol. 137: 1183-1188 (2011) https://doi.org/10.1016/j.jep.2011.07.050
  7. Faria A, Pestana D, Teixeira D, Couraud PO, Romero I, Weksler B, de Freitas V, Mateus N, Calhau C. Insights into the putative catechin and epicatechin transport across blood-brain barrier. Food Funct. 2: 39-44 (2011) https://doi.org/10.1039/C0FO00100G
  8. Ferguson CA, Kidson SH. The regulation of tyrosinase gene transcription, Pigment Cell Res. 10: 127 (1997) https://doi.org/10.1111/j.1600-0749.1997.tb00474.x
  9. Florence NT, Benoit MZ, Jonas K, Alexandra T, Dsir DD, Pierre K, Thophile D. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced dia-betic rats. J. Ethnopharmacol. 151: 784-790 (2013) https://doi.org/10.1016/j.jep.2013.09.021
  10. Flurkey WH. Identification of tyrosinase in mushrooms by isoelectric focusing. J. Food Sci. 56: 93-95 (1991) https://doi.org/10.1111/j.1365-2621.1991.tb07983.x
  11. Han GS, Kim DS, Woo WH, Mun YJ. Inhibitory effect of Fructus ligustri lucidi on tyrosinase and MITF expressions. Kor. J. Ori. Physiol. Pathol. 24: 296-301 (2010)
  12. Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 5: 2902 (1991) https://doi.org/10.1096/fasebj.5.14.1752358
  13. Hibasami H, Komiya T, Achiwa Y, Ohnishi K, Kojima T, Nakanishi K, Akashi K, Hara Y. Induction of apoptosis in human stomach cancer cells by green tea catechins. Oncol Rep. 5: 527-529 (1998)
  14. Jang JY, Kim HN, Kim YR, Kim BW, Choi YH, Choi BT. Studies of Inhibitory Mechanism on Melanogenesis by Partially Purified Asiasari radix in ${\alpha}$-MSH Stimulated B16F10 Melanoma Cells. J. Life Sci. 20: 1617-1624 (2015) https://doi.org/10.5352/JLS.2010.20.11.1617
  15. Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, Hearing VJ, Lozano JA, Garcia-Borron JC. A new enzymatic function in the melanogenic pathway. The 5, 6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J. Biol. Chem. 269: 17993-18000 (1994)
  16. Joo D, Jeong S, Lee HK, Shin SH, Choi SJ, Kim K, An IS, Kim KY, Ku JE, Jeong SH, Cha HJ. Annona muricata L. extracts decrease melanogenesis in B16F10 mouse melanoma cells. Biomedical Dermatology. 1: 10-16 (2017) https://doi.org/10.1186/s41702-017-0010-7
  17. Kang MK, Lee YE, Woo WH, Mun YJ. Commelina communis Ledeb inhibitis melanin synthesis in alpha-MSH-stimulated B16F10 cells. J. Physiol. Pathol. Korean Med. 28: 506-511 (2014)
  18. Kim YE, Lee JH, Sung NY, Ahn DH, Byun EH. A comparative study of the immuno-modulatory activities of ethanol extracts and crude polysaccharide fractions from Annona muricata L. Korean J. Food Sci. Technol. 4: 453-458 (2017)
  19. Moghadamtousi SZ, Rouhollahi E, Hajrezaie M, Karimian H, Abdulla MA, Kadir HA. Annona muricata leaves accelerate wound healing in rats via involvement of Hsp70 and antioxidant defence. Int. J. Surg. 18: 110-117 (2015a) https://doi.org/10.1016/j.ijsu.2015.03.026
  20. Moghadamtousi SZ, Rouhollahi E, Karimian H, Fadaeinasab M, Firoozinia M, Ameen Abdulla M, Abdul Kadir H. The chemopotential effect of Annona muricata leaves against azoxymethaneinduced colonic aberrant crypt foci in rats and the apoptotic effect of acetogenin annomuricin E in HT-29 cells: a bioassayguided approach. PLoS One 10: 10-14 (2015b)
  21. No JK, Soung DY, Kim YJ, Shim KH, Jun YS, Rhee SH, Yokozawa T, Chung HY. Inhibition of tyrosinase by green tea components. J. Life. Sci. 65: 241-246 (1999)
  22. Nouemo P, Koudogbo B, Tchivounda H, Nguema C, Etoua M. Effects of ethanol extract of Annona muricata on pentylenetetrazol-induced convulsive seizures in mice, Phytother. Res. 11: 243-245 (1997) https://doi.org/10.1002/(SICI)1099-1573(199705)11:3<243::AID-PTR66>3.0.CO;2-A
  23. Park SH, Kim AH, Park BJ, Kim JJ. Whitening effect of Biochanin A. J. Soc. Cosmet. Sci. Korea. 39: 289-294 (2013) https://doi.org/10.15230/SCSK.2013.39.4.289
  24. Yoo DH, Joo DH, Lee SY, Lee JY. Antioxidant effect of Nelumbo nucifera G. leaf extract and inhibition of MITF, TRP-1, TRP-2, and tyrosinase expression in a B16F10 melanoma cell line. J. Life Sci. 25: 1115-1123 (2015) https://doi.org/10.5352/JLS.2015.25.10.1115
  25. Yuan SS, Chang HL, Chen HW, Yeh YT, Kao YH, Lin KH, Wu YC, Su JH. Annonacin, a mono-tetrahydrofuran acetogenin, arrests cancer cells at the G1 phase and causes cytotoxicity in a Bax-and caspase-3-related pathway. Life Sci. 72: 2853-2861 (2003) https://doi.org/10.1016/S0024-3205(03)00190-5
  26. Zahra TH, Akaberi M, Vatani M, Emami SA. Evaluation of antioxidant and anti-melanogenic activities of different extracts from aerial parts of Nepeta binaludensis Jamzad in murine melanoma B16F10 cells, Iran. J. Basic Med. Sci. 19: 662-669 (2016)