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Antimelanogenic effect and whitening of crude polysaccharide fraction extracted from Perilla frutescens Britton var. acuta Kudo

자소엽(Perilla frutescens Britton var. acuta Kudo) 조다당의 멜라닌 생성 저해 및 미백효과

  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 조은지 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.10.17
  • Accepted : 2019.01.22
  • Published : 2019.02.28

Abstract

In this study, the inhibitory effects of crude polysaccharide fractions separated from Perilla frutescens Britton var. acuta Kudo (PCP) on melanin synthesis and tyrosinase activity were observed. B16F10 melanoma cells were treated with 125 and $250{\mu}g/mL$ of PCP for 24 hours. Using these optimal concentrations, inhibition of melanin synthesis inhibition was measured, and PCP treatment significantly reduced melanin synthesis induced by 3-isobutyl-1-methylxanthine (IBMX). In addition, western blotting analysis on B16F10 melanoma cells showed that PCP inhibited tyrosinase, microphthalmia-associated transcriptipn factor, tyrosinase related protein-1, and tyrosinase related protein-2 expression. Therefore, these results indicate that PCP may have potential inhibitory activity against melanin synthesis and may be a natural ingredient useful for the development of whitening materials in cosmetics and functional foods.

Keywords

Perilla frutescens Britton var. acuta Kudo;tyrosinase;tyrosinase related protein-1;tyrosinase related protein-2;microphthalmia-associated transcription factor

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Fig. 1. Tyrosinase inhibition activity of crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP).

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Fig. 2. Cell viability of IBMX and crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP) in B16F10 melanoma cells.

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Fig. 3. The difference of melanin octents in B16F10 mouse melanoma cells treated with crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP).

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Fig. 4. Effect of crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP) on cellular melanin contents and tyrosinase activity in B16F10 cells.

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Fig. 5. Inhibitory effect of fractions from crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP) on TRP-1, TRP-2, MITF, Tyrosine.

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