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Anti-obesity effect of 3,5-dicaffeoylquinic acid on high-fat diet mouse

고지방식이 마우스에서 3,5-dicaffeoylquinic acid의 항비만 효과

  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Su Bin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Yoo, Seul Ki (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Han, Hye Ju (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Dae Ok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 강진용 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 박수빈 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 유슬기 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 한혜주 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 김대옥 (경희대학교 식품생명공학과) ;
  • 허호진 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원)
  • Received : 2018.11.16
  • Accepted : 2018.12.30
  • Published : 2019.02.28

Abstract

This study was performed to confirm the influence of chlorogenic acid (CGA) and 3,5-dicaffeyolquinic acid (3,5-diCQA) intake on problems caused by high-fat diet. CGA was more effective in suppressing weight gain than 3,5-diCQA. In contrast, 3,5-diCQA was more effective in improving glucose tolerance than CGA. In the biopsy, it was confirmed that CGA inhibited visceral fat and liver fat accumulation. 3,5-diCQA also inhibited visceral fat accumulation, but 3,5-diCQA increased liver fat accumulation. The liver fat accumulation induced oxidative stress, but 3,5-diCQA reduced oxidative damage through its antioxidant activity. The increased liver fat accumulation was because a 3,5-diCQA greatly increased Akt phosphorylation and decreased AMPK phosphorylation in the liver. Consequently, CGA was effective in alleviating the problems caused by high-fat diets, while maintaining normal balance. 3,5-diCQA also showed a positive effect on problems caused by high-fat diets, but it increased liver fat accumulation and thereby had negative consequences.

Keywords

3,5-dicaffeoylquinic acid;chlorogenic acid;obesity;p-AMPK;p-Akt

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Fig. 1 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on body weight change.

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Fig. 2 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on glucose tolerance.

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Fig. 3 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on lipid accumulation.

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Fig. 4 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on liver damage.

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Fig. 5 The effect of CGA and 3,5-diCQA intake HFD-induced obese mice on protein expression of p-AMPK/β-actin in epididymal adipose (A) and liver tissue (B) and p-Akt/β-actin in epididymal adipose (C) and liver tissue (D) and representative image (E).

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Fig. 5. Continued

Table 1. Effect of CGA and 3,5-diCQA on body weight changes and food intake in HFD-induced obese mice

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Table 2. Effect of CGA and 3,5-diCQA on serum biomarker in HFD-induced obese mice

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Table 3. Effect of CGA and 3,5-diCQA on serum lipid biomarker in HFD-induced obese mice

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Acknowledgement

Supported by : 한국연구재단

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