Survey of bovine norovirus infections from diarrheic calves in South Korea, 2015-2017

  • Lee, Eun-Yong (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kang, Hyung-Woo (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kim, Seong-Hee (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Moon, Boyoun (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • So, Byung Jae (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Lee, Kyoung-Ki (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kim, Yeon-Hee (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
  • Received : 2018.11.01
  • Accepted : 2019.02.18
  • Published : 2019.03.31


This study examined complex infections with various enteropathogens and the genetic diversity of bovine norovirus (BNoV) in 932 fecal samples from diarrheic calves in South Korea. Overall, seventeen (1.8%) of the samples tested positive for BNoV following RT-PCR examination. All BNoV-positive samples were co-infected with other intestinal pathogens, including bovine Rotavirus, Giardia, Cryptosporidium, and Escherichia coli. The genetic diversity of the BNoVs shared high nucleotide identity (98.1-99.5%) and amino acid homology (93.5-98.1%) with genotype 2 BNoV (GIII.2) strains. In conclusion, BNoV infections with GIII genotypes were detected in complex infections of diarrheic calves in South Korea.

Caliciviruses, belonging to the family Caliciviridae, are non-enveloped, single-stranded RNA viruses with positive-sense genomes of 7.4–8.3 kb. Based on genomic organization and genetic analysis [1], the Caliciviridae have been divided into at least four genera: Vesivirus, Lagovirus, Norovirus (NoV), and Sapovirus as well as a proposed genus, NB-like [2]. Phylogenetically, noroviruses (NoVs) are divided into five genogroups (GI–GV). Human are infected by GI, GII, and GIV NoVs [3], whereas animal NoVs are predominantly categorized as GII (swine), GIII (ruminants), GIV (lions and dogs), and GV (mice) based on the nature of the species infection [4]. NoVs comprise the leading cause of acute nonbacterial gastroenteritis in humans; notably genetically related viruses have been isolated in cattle feces. Currently, five genogroups have been explicated in the genus Norovirus. Depending on genetic homology and phylogenetic relationships, those genogroups have been further subdivided into genotypes. All bovine noroviruses (BNoVs) occupy genogroup III (GIII); thus far, genotype 1 (GIII.1) (Jena/1980/DE) and genotype 2 (GIII.2) (Newbury2/1976/UK) BNoV genomes have been fully sequenced. Among the three open reading frames (ORFs) of the BNoV genome, ORF1 encodes a polyprotein that is processed into six nonstructural proteins (N-terminal protein, NTPase, 3A-like protein, viral genome-linked protein [VPg], 3C-like proteinase, and polymerase). Furthermore, ORF2 encodes a single capsid protein, whereas ORF3 coordinates a minor structural protein [5]. In this study, we examined the presence of BNoVs in Korean calves by performing reverse transcription-polymerase chain reaction (RT-PCR) to determine the genetic diversity of Korean BNoV isolates. During diagnostic examinations of BNoV infection by RT-PCR, capsid protein sequences were obtained from diarrheic fecal samples, allowing comparisons with previously reported reference strains [6]. A total of 932 bovine diarrheic fecal samples were submitted to APQA from January 2015 to August 2017. Norovirus primers (BNoV-F/BNoV-R), which amplify a 515 bp partial gene of the capsid protein of BNoV GIII and have the following sequences: BNoV-F (5'-CGCTCCATGTTYGCBTGG-3') and BNoV-R (5'-ATCAGCACATGRGGRAACTG-3'), and Maxime RT-PCR premix (Intron, Korea) were used for the detection of noroviruses, as described previously for Korean diarrheic cattle [7]. Positive DNA amplicons (515 nucleotides) were directly sequenced by an ABI Prism 3730XI DNA Sequencer (Applied Biosystems, USA) at the Macrogen Institute (Korea). Comparative analysis of nucleotide sequences was performed by using BioEdit Sequence Editor Version 7.1 software and Molecular Evolutionary Genetic Analysis (MEGA) version 6.0 with bootstrap values calculated from 1000 replicates [8]. The neighbor-joining analysis was used to construct the phylogenetic tree by applying the maximum likelihood method [9]. All diarrheic fecal samples were also tested for the presence of other pathogens, including bovine Rotavirus (BRV), bovine viral diarrhea virus (BVD), bovine Kobuvirus (Kobu), Giardia  (GIAR), Cryptosporidium  (CRYP), enterohemorrhagic Escherichia coli (EHEC), and Clostridium difficile (CDIFF) [10].

Among the 932 diarrheic fecal samples, 17 (1.8%) were positive for BNoV. The 17 RT-PCR products were sequenced and analyzed phylogenetically to confirm their nucleotide identity with previously reported BNoV strains belonging to genotype GIII (GenBank accession numbers: MH579349– MH579361). The 17 Korean strains showed 98.1%–99.5% nucleotide identity and 93.5%–98.1% amino acid homology with the GIII.2 reference Bo/Newbury2/UK strain (Fig. 1). The ages of the infected calves were between 3 and 40 days. The numbers of BNoV-positive calves at specific ages were as follows: seven at 3–7 days old, five at 8−14 days old, three at 15–21 days old, and two at 22–40 days old. Other bovine enteric pathogens might have important roles in diarrhea symptoms, both clinically and pathologically, because many other intestinal pathogens have been detected in cattle diarrhea [11]. Of the other enteric pathogens tested in this study, BRV was the predominant agent of co-infection with BNoV (41.2%); the remaining pathogens present were GIAR (23.5%), CRYP (23.5%), Kobu (23.5%), BVD (17.6%), EHEC (11.8%), and CDIFF (11.8%). Interestingly, two diarrheic fecal samples tested positive for the presence of four enteric pathogens (Table 1).

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Fig. 1. A phylogenetic tree was constructed on the basis of the partial nucleotide sequence of the capsid protein of caliciviruses by using the maximum likelihood method. GenBank accession numbers (MH579349–MH579361) are registered, except for four identical sequences. The bootstrap value (percent) is given at each node. Viruses for which capsid gene sequences were used for phylogenetic analysis are included. Phylogenetically, noroviruses are divided into five genogroups (GI−GV).

Table 1. Summary of the enteric pathogens present in diarrheic fecal specimens obtained from calves

20200713_140134.jpg 이미지

Based on comparisons of the amino acid and nucleotide sequences of the capsid protein fragment, 17 BNoV strains belonging to GIII.2 were detected in diarrheic feces of calves in South Korea. Genotypes 1 and 2 were reported in the first Korean domestic report of BNoV in diarrhea samples that were collected from 2004 to 2005; however, the present results showed that only genotype 2 (GIII.2) was present during sample collection from 2015 to 2017. Notably, the detection rate of BNoV infections in diarrheic calves was reported to be 9.3% during collections from 2004 to 2005 in South Korea [11]. However, no further reports were published until 2017. In other countries, the following BNoV infection rates have been reported: 8.0% in England [12], 21% in Italy [13], 31.6% in Netherlands [14], and 72.0% in Ohio, USA [2]. BNoV infections were detected in Turkey at a rate of 1.7% and were identical to 100% Italian and Tunisian strains [15]. Based on the RT-PCR analysis, BNoVs were detected in 1.8% of the fecal specimens examined in this study. These results suggest that the BNoV-positive rate has decreased markedly; moreover, the mean positive rate has decreased since the initial report of 9.3% in South Korea. Park et al. [11] reported BNoV infection with only other enteric viruses; however, NoV can participate in a complex infection, which can be shown by simultaneous testing for other intestinal pathogenic microorganisms including bacteria, protozoa and parasites. In this study, we also observed that there was a difference in the BNoV-positive rate according to calf age with the BNoV-positive rate decreasing with age.

This study confirmed the presence of one single genotype of BNoV, the GIII type, and showed that the GIII.2 BNoV was the predominant genotype; results that are consistent with the previously reported BNoV genogroup and genotype results. Additional pathogenicity studies into single and multiple infections are needed as these BNoVs form endemic infections in diarrheic calves in South Korea.


This project was supported by a grant (N-1543069-201599-03) from the Animal and Plants Quarantine Agency (APQA), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.


Supported by : Animal and Plants Quarantine Agency (APQA)


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