Soybean meal substitution by dehulled lupine (Lupinus angustifolius) with enzymes in broiler diets

  • Mera-Zuniga, Fredy (Program of Animal Science, Postgraduate College-Campus Montecillo) ;
  • Pro-Martinez, Arturo (Program of Animal Science, Postgraduate College-Campus Montecillo) ;
  • Zamora-Natera, Juan F (Department of Botany and Zoology, University Center of Biological and Agricultural Sciences, University of Guadalajara) ;
  • Sosa-Montes, Eliseo (Department of Animal Husbandry, Autonomous University of Chapingo) ;
  • Guerrero-Rodriguez, Juan D (Postgraduate College-Campus Puebla) ;
  • Mendoza-Pedroza, Sergio I (Department of Animal Husbandry, Autonomous University of Chapingo) ;
  • Cuca-Garcia, Juan M (Program of Animal Science, Postgraduate College-Campus Montecillo) ;
  • Lopez-Romero, Rosa M (Program of Soil Science, Postgraduate College-Campus Montecillo) ;
  • Chan-Diaz, David (Trouw Nutrition Mexico) ;
  • Becerril-Perez, Carlos M (Program of Animal Science, Postgraduate College-Campus Montecillo) ;
  • Vargas-Galicia, Artemio J (Program of Animal Science, Postgraduate College-Campus Montecillo) ;
  • Bautista-Ortega, Jaime (Program in Bioprospecting and Agricultural Sustainability in the Tropics, Postgraduate College-Campus Campeche)
  • Received : 2018.05.01
  • Accepted : 2018.08.29
  • Published : 2019.04.01


Objective: Evaluate the effects of i) dehulling of lupine seed on chemical composition and apparent metabolizable energy (AME) and ii) soybean meal substitution by dehulled lupine seed in broiler diets with enzymes on productive performance, size of digestive organs and welfare-related variables. Methods: Experiment 1, chemical composition and AME were determined in whole and dehulled lupine seed. Experiment 2, two hundred eighty-eight one-day-old male Ross 308 broilers were used. The experimental diets were maize-soybean meal (MS), MS with enzymes (MSE) and maize-dehulled lupine seed with enzymes (MLE). Diets were assigned to the experimental units under a completely randomized design (eight replicates per diet). The body weight (BW) gain, feed intake, feed conversion, digestive organ weights, gait score, latency to lie down and valgus/varus angulation were evaluated. Results: The dehulling process increased protein (25.0% to 31.1%), AME (5.9 to 8.8 MJ/kg) and amino acid contents. The BW gain of broilers fed the MLE diet was similar (p>0.05) to that of those fed the MS diet, but lower than that of those fed the MSE diet. Feed intake of broilers fed the MLE diet was higher (p<0.05) than that of those fed the MS diet and similar (p>0.05) to those fed the MSE diet. Feed conversion of broilers fed the MLE diet was 8.0% and 8.7% higher (p<0.05) than that of those fed the MS and MSE diets, respectively. Broilers fed the MLE diet had the highest (p<0.05) relative proventriculus and gizzard weights, but had poor welfare-related variables. Conclusion: It is possible to substitute soybean meal by dehulled lupine seed with enzymes in broiler diets, obtaining similar BW gains in broilers fed the MLE and MS diets; however, a higher feed intake is required. Additionally, the MLE diet reduced welfare-related variables.


Apparent Metabolizable Energy;Enzymes;Welfare-Related Variables;Broiler Performance;Dehulled Lupine;Digestive Organ Size


Supported by : National Council of Science and Technology of Mexico (CONACyT)


  1. Lee MRF, Parkinson S, Fleming HR, et al. The potential of blue lupins as a protein source, in the diets of laying hens. Vet Anim Sci 2016;1-2:29-35.
  2. Perween S, Kumar K, Chandramoni, et al. Effect of feeding different dietary levels of energy and protein on growth performance and immune status of Vanaraja chicken in the tropic. Vet World 2016;9:893-9.
  3. Francesch M. Sistemas para la valoracion energetica de los alimentos en aves. Arch Latinoam Prod Anim 2001;9:35-42.
  4. Vecerek V, Suchy P, Strakova E, Machacek M. Nutritive Composition of seeds of the lupin varieties registered in the Czech Republic. In: Palta JA, Berger JD, editors. Lupins for health and wealth. Proceedings of the 12th International Lupin Conference, 14-18 Sept. 2008, Fremantle, Western Australia. International Lupin Association, Canterbury, New Zealand; 2008. pp. 123-6.
  5. Mierlita D, Popovici D. Effect of partial substitution of soybean meal with lupine seeds on growth and economic efficiency of broilers. Lucrari Stiintifice-Seria Zootehnie 2013;59:60-5.
  6. Nalle CL, Ravindran V, Ravindran G. Nutritional value of narrow-leafed lupin (Lupinus angustifolius) for broilers. Br Poult Sci 2011;52:775-81.
  7. Evans AJ, Cheung PC-K, Cheetham NWH. The carbohydrate composition of cotyledons and hulls of cultivars of Lupinus angustifolius from Western Australia. J Sci Food Agric 1993;61:189-94.
  8. Pasquali GAM, Fascina VB, Silva AL, et al. Maize replacement with sorghum and a combination of protease, xylanase, and phytase on performance, nutrient utilization, litter moisture, and digestive organ size in broiler chicken. Can J Anim Sci 2017;97:328-37.
  9. AOAC. Official methods of analysis of the Association of Official Analytical Chemists. 15th ed. Washington, DC, USA: Association of Official Analytical Chemists; 1990.
  10. Aviagen. Ross broiler nutrition supplement [internet]. Newbridge, UK: Aviagen Ltd; 2014. Available from:
  11. Official Mexican Standard, NOM-033-SAG/ZOO-2014. Methods to kill domestic and wild animals. Official Diary of the Federation, Mexico; 2015.
  12. Garner JP, Falcone C, Wakenell P, Martin M, Mench JA. Reliability and validity of a modified gait scoring system and its use in assessing tibial dyschondroplasia in broilers. Br Poult Sci 2002;43:355-63.
  13. Leterrier C, Nys Y. Clinical and anatomical differences in varus and valgus deformities of chick limbs suggest different aetiopathogenesis. Avian Pathol 1992;21:429-42.
  14. Berg C, Sanotra GS. Can a modified latency-to-lie test be used to validate gait-scoring results in commercial broiler flocks? Anim Welf 2003;12:655-9.
  15. Sujak, A, Kotlarz A, Strobel W. Compositional and nutritional evaluation of several lupin seeds. Food Chem 2006;98:711-9.
  16. Kingwell R. Extracting value from protein variation in lupins. In: Glencross BD, editor. Proceedings of the third workshop for Seeding a Future for Grains in Aquaculture Feeds- 14 April 2005, Fisheries Occasional Publications No. 24, Department of Fisheries, Western Australia, Australia; 2013. pp. 17-9.
  17. Wideman RF, Rhoads DD, Erf GF, Anthony NB. Pulmonary arterial hypertension (ascites syndrome) in broilers: a review. Poult Sci 2013;92:64-83.
  18. Nadal S, Moreno MT, Cubero JI. The grain legumes in modern agriculture. 1st ed. Madrid, Spain: Editions Mundi-Prensa; 2004.
  19. Nalle CL, Ravindran G, Ravindran V. Influence of dehulling on the apparent metabolisable energy and ileal amino acid digestibility of grain legumes for broilers. J Sci Food Agric 2010;90:1227-31.
  20. Brenes A, Marquardt RR, Guenter W, Viveros A. Effect of enzyme addition on the performance and gastrointestinal tract size of chicks fed lupin seed and their fractions. Poult Sci 2002;81:670-8.
  21. Svihus B. Function of the digestive system. J Appl Poult Res 2014;23:306-14.
  22. Shirzadegan K, Taheri HR. Insoluble fibers affected the performance, carcass characteristics and serum lipid of broiler chickens fed wheat-based diet. Iranian J Appl Anim Sci 2017;7:109-17.
  23. Rubio LA, Brenes A, Centeno C. Effects of feeding growing broiler chickens with practical diets containing sweet lupin (Lupinus angustifolius) seed meal. Br Poult Sci 2003;44:391-7.
  24. Józefiak D, Rutkowski A, Jensen BB, Engberg RM. Effects of dietary inclusion of triticale, rye and wheat and xylanase supplementation on growth performance of broiler chickens and fermentation in the gastrointestinal tract. Anim Feed Sci Technol 2007;132:79-93.
  25. Nalle CL, Ravindran V, Ravindran G. Nutritional value of white lupins (Lupinus albus) for broilers: apparent metabolisable energy, apparent ileal amino acid digestibility and production performance. Animal 2012;6:579-85.
  26. Olkowski BI, Classen HL, Wojnarowicz C, Olkowski AA. Feeding high levels of lupine seeds to broiler chickens: plasma micronutrient status in the context of digesta viscosity and morphometric and ultrastructural changes in the gastrointestinal tract. Poult Sci 2005;84:1707-15.
  27. Su G, Sorensen P, Kestin SC. A note on the effects of perches and litter substrate on leg weakness in broiler chickens. Poult Sci 2000;79:1259-63.
  28. Dawkins MS, Donnelly CA, Jones TA. Chicken welfare is influenced more by housing conditions than by stocking density. Nature 2004;427:342-4.
  29. Caprita R, Caprita A, Julean C. Biochemical aspects of nonstarch polysaccharides. Scientific papers: Anim Sci Biotechnol 2010;43:368-75.
  30. Bach Knudsen KE. The nutritional significance of "dietary fibre" analysis. Anim Feed Sci Technol 2001;90:3-20.