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Changes in expression of the autophagy-related genes microtubule-associated protein 1 light chain 3β and autophagy related 7 in skeletal muscle of fattening Japanese Black cattle: a pilot study

  • Nakanishi, Tomonori (Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki) ;
  • Tokunaga, Tadaaki (Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki) ;
  • Ishida, Takafumi (Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki) ;
  • Kobayashi, Ikuo (Sumiyoshi Livestock Science Station, Field Science Center, University of Miyazaki) ;
  • Katahama, Yuta (Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki) ;
  • Yano, Azusa (Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki) ;
  • Erickson, Laurie (Department of Biology, Harold Washington City College of Chicago) ;
  • Kawahara, Satoshi (Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki)
  • Received : 2018.05.09
  • Accepted : 2018.09.03
  • Published : 2019.04.01

Abstract

Objective: Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain $3{\beta}$ (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth. Methods: Six castrated Japanese Black cattle (initial body weight: $503{\pm}20kg$) were enrolled in this study and fattened for 7 months. Three skeletal muscles, M. longissimus, M. gluteus medius, and M. semimembranosus, were collected by needle biopsy three times during the observation period, and mRNA levels of MAP1LC3B and ATG7 were determined by quantitative reverse-transcription polymerase chain reaction. The expression levels of genes associated with the ubiquitin-proteasome system, another proteolytic mechanism, were also analyzed for comparison with autophagy-related genes. In addition, ultrasonic scanning was repeatedly performed to measure M. longissimus area as an index of muscle growth. Results: Our results showed that both MAP1LC3B and ATG7 expression increased over the observation period in all three skeletal muscles. Interestingly, the increase in expression of these two genes in M. longissimus was highly correlated with ultrasonic M. longissimus area and body weight. On the other hand, the expression of genes associated with the ubiquitin-proteasome system was unchanged during the same period. Conclusion: These findings suggest that autophagy plays an important role in the growth of skeletal muscle of fattening beef cattle and imply that autophagic activity affects meat productivity.

Keywords

Autophagy;Microtubule-associated Protein 1 Light Chain $3{\beta}$ (MAP1LC3B);Autophagy Related 7 (ATG7);Ultrasonic Scanning;Cattle;Skeletal Muscle Growth

Acknowledgement

Supported by : Japan Society for the Promotion of Science

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