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보골탕이 Monosodium Iodoacetate 유도 골관절염과 Interleukin-1β 유도 연골세포에 미치는 보호 효과

Protective Effects of Bogol-tang on Monosodium Iodoacetate-induced Osteoarthritis and Interleukin-1β-treated Primary Chondrocytes

  • 성진욱 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 이해웅 (동의대학교 한의과대학 예방의학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 김경민 (동의대학교 한의과대학 한방내과학교실) ;
  • 조성우 (동의대학교 한의과대학 한방재활의학과교실)
  • Sung, Jin Wook (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dong-eui University) ;
  • Lee, Hai Woong (Department of Preventive Medicine, College of Korean Medicine, Dong-eui University) ;
  • Kang, Kyung Hwa (Department of Physiology, College of Korean Medicine, Dong-eui University) ;
  • Kim, Kyoung Min (Department of Oriental Internal Medicine, College of Korean Medicine, Dong-eui University) ;
  • Cho, Sung Woo (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dong-eui University)
  • 투고 : 2019.03.14
  • 심사 : 2019.04.17
  • 발행 : 2019.04.30

초록

Objectives Bogol-tang has clinically been used to protect joint cartilage and to treat osteoarthritis. Our objective was to study the protective effect of Bogol-tang extract (BGT) in functional impairment, behavioral disorders, cartilage loss and pathological changes in a monoiodoacetate (MIA)-induced murine osteoarthritis (OA) model and interleukin (IL)-$1{\beta}$ -treated primary rat chondrocytes. Methods Mouse knee joints were injected with MIA, a chemical that inhibits glycolysis and causes joint inflammation and matrix loss. MIA-OA induced mice orally administered BGT or acetaminophen (AAP) for 18 days by daily. Primary rat chondrocytes were pretreated with BGT or dexamethasone (DEX) and followed by co-incubation with IL-$1{\beta}$ (10 ng/mL). Results In MIA-OA mice model, BGT led to delayed response on hot plate analysis, and suppressed the cartilage loss and damages in joint tissues. BGT suppressed the elevated levels of inflammatory mediators, nitrite and $PGE_2$, the gene expression of matrix degrading enzymes, and extracellular-signal-regulated kinases 1/2 and c-JunN-terminal kinase phosphorylation in IL-$1{\beta}$-treated primary rat chondrocytes. Conclusions Our results suggest that BGT improve the knee joint function and delay the cartilage damages by anti-nociceptive, anti-inflammatory and ant-catabolic effects, which indicate BGT could be a potential candidate for osteoarthritis treatment.

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