DOI QR코드

DOI QR Code

Effects of Curcumae Longae Rhizoma Pharmacopuncture on Monosodium Iodoacetate-induced Osteoarthritis Rats

강황(薑黃) 약침이 Monosodium Iodoacetate 유도 골관절염 흰쥐에 미치는 영향

  • Lee, Jong-Hoon (Department of Rehabilitaion Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Woo, Chang-Hoon (Department of Rehabilitaion Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University)
  • 이종훈 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Received : 2019.01.17
  • Accepted : 2019.04.03
  • Published : 2019.04.30

Abstract

Objectives The purpose of this study was to evaluate the effects of Curcumae Longae Rhizoma pharmacopuncture on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods Osteoarthritis was induced by injection of MIA ($50{\mu}L$ with 80 mg/mL) into knee joint cavity of rats. Rats were divided into 6 groups. Normal group was injected by normal saline into knee joint cavity only. Control group was induced for osteoarthritis by MIA and orally administered with distilled water. Normal Saline group was induced for osteoarthritis by MIA and injected with normal saline $100{\mu}L$. Positive comparison group was injected with MIA and orally administered with indomethacin 5 mg/kg. Curcumae Longae Rhizoma pharmacopuncture low concentration (CL) group was induced for osteoarthritis by MIA and injected with Curcumae Longae Rhizoma pharmacopuncture low concentration $100{\mu}L$. Curcumae Longae Rhizoma pharmacopuncture high concentration (CH) group was induced for osteoarthritis by MIA and injected with Curcumae Longae Rhizoma pharmacopuncture high concentration $100{\mu}L$. Curcumae Longae Rhizoma pharmacopuncture was injected at ST35 and EX-LE4 each group (CL, CH). After that, hind paw weight distribution was measured and oxidative stress biomarker in serum, liver function biomarker in serum, western blot analysis were measured. Histological analysis of knee joint tissue was performed by hematoxylin and eosin staining, Safranin-O staining and Masson's trichrome staining. Results Hind paw weight distribution was significantly improved in both group. alanine aminotransferanse and aspartate aminotransferase were decreased significantly in CH group compare with Indomethacin threated group. Antioxidant enzyme glutathione peroxidase, Catalase and heme oxygenase-1 were increased in CH group compare with control group. Inflammatory cytokine cyclooxygenase-2, inducible nitric oxide synthase and interleukin-1 beta were decreased significantly in CH group. Histological analysis result shows that protective effects of joint and cartilage were observed in both CH and CL groups in a concentration-dependent. Conclusions The result suggest that Curcumae Longae Rhizoma pharmacopuncture has anti-oxidation effect, anti-inflammatory effect and also can prevent progression of osteoarthritis and protect joint cartilage.

References

  1. The Society of Korean Medicine Rehabilitation. Korea Rehabilitation Medicine. 4th ed. Paju:Koonja Publishing. 2015:102-16.
  2. The Korean Orthopaedic Association. Orthopaedics. 7th ed. Seoul:Choisin medical Publishing Co. 2013:49-50, 99-100, 317-26.
  3. Jung IC, Kim ES, Lee EJ, Gu JH, Park YC. A systematic review of bee venom acupuncture for knee osteoarthritis. J Korean Med Rehabil. 2017;27(3):47-60. https://doi.org/10.18325/jkmr.2017.27.3.47
  4. Science Commitee of Korean Pharmacopuncture Institute, Pharmacopuncture Medicine Labaratory. Pharmacopuncturology. Seoul:Elsevier Korea. 2011:3-8.
  5. A Herbal Medicine Compilation Committee of a College of Korean Medicine. Herbal medicine. 3th ed. Seoul: Yeonglimsa. 2014:454-6.
  6. Yoon JH, Ryu BH, Kim JS, Yoon SH. Effects of Curcuma longa L. on some kinds of cancer cells. The Journal of Internal Korean Medicine. 2006;27(2):429-43.
  7. Jung SY, Oh MS, Ju MS, Oh HI, Park HB. Comparative study of anti-oxidant and anti-inflammatory activities between Curcumae Longae Radix and Curcumae Longae Rhizoma. Korea Journal of Herbology. 2010;25(1):83-91. https://doi.org/10.6116/KJH.2010.25.1.083
  8. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med. 1994;16(2):149-56. https://doi.org/10.1016/0891-5849(94)90138-4
  9. Shane Anderson A, Loeser RF. Why is osteoarthritis an age related disease? Best Pract Res Clin Rheumatol. 2010; 24(1):15-26. https://doi.org/10.1016/j.berh.2009.08.006
  10. Ministry of Health and Welfare. Korea Health Statistics 2012: Korea National Health and Nutrition Examination Survey [Internet] 2013 [cited 2017 Oct 11]. Available from: URL: https://knhanes.cdc.go.kr/knhanes/sub04/sub04_03.do?classType=7.
  11. Fauci AS, Kasper DL, Longo DL, Braunwald E, Hauser SL, Jameson JL, Loscalzo J. Harrison's Principles of Internal Medicine. 17th ed. Seoul:Doseochulpah MIP. 2010:2594-603.
  12. Wolfe MM, Lichtenstein DR, Singh G. Gastrointestinal toxicity of nonsteroidal antiinflammatory drugs. N Engl J Med. 1999;340(24):1888-99. https://doi.org/10.1056/NEJM199906173402407
  13. Mukherjee D, Nissen SE, Topol EJ. Risk of cardiovascular events associated with selective COX-2 inhibitors. J Am Med Assoc. 2001;286(8):954-9. https://doi.org/10.1001/jama.286.8.954
  14. Textbook Committee of Korean Acupuncture and Moxibustion Society. Acupuncture Medicine. 4th ed. Seoul: Hanmi Medical Publishing Co. 2016:550.
  15. Kim WY, Choi JB. Effects of bee venom and Cervi Cornu Parvum pharmacoacupuncture in monosodium iodoacetate( MIA)-induced osteoarthritis rat. J Korean Med Rehabil. 2010;20(1):61-77.
  16. Kim YH, Yim YK, Lee H. The effects of herbal-acupuncture with Aconiti iateralis preparata Radix solutionon collagen- induced arthritis. Korean J Acupunct. 2006;23(2):137-54.
  17. Lee HJ, Kim JS, Lim SC, Lee YK, Ji MJ. Original article: effects of Curculiginis rhizoma pharmaco-acupuncture at ST36 on monosodium iodoacetate(MIA)-induced osteoarthritic rats. Journal of Acupuncture Research. 2015;32(1): 53-66. https://doi.org/10.13045/acupunct.2015005
  18. Deshpande UR, Gadre SG, Raste AS, Pillai D, Bhide SV, Samuel AM. Protective effect of turmeric (Curcuma longa L.) extract on carbon tetrachloride-induced liver damage in rats. Indian Journal of Experimental Biology. 1998; 36(6):573-7.
  19. Kim DC, Kim SH, Choi BH, Baek NI, Kim D, Kim MJ, Kim KT. Curcuma longa extract protects against gastric ulcers by blocking H2 histamine receptors. Biol Pharm Bull. 2005;28(12):2220-4. https://doi.org/10.1248/bpb.28.2220
  20. Yadav SK, Sah AK, J ha RK, S ah P , Shah DK. Turmeric (curcumin) remedies gastroprotective action. Pharmacogn Rev. 2013;7(13):42-6. https://doi.org/10.4103/0973-7847.112843
  21. Funk JL, Frye JB, Oyarzo JN, Kuscuoglu N, Wilson J, McCaffrey G, Stafford G, Chen G, Lantz RC, Jolad SD, S olyom AM, Kiela PR, Timmermann BN. Efficacy and mechanism of action of turmeric supplements in the treatment of experimental arthritis. Arthritis Rheum. 2006;54(11):3452-64. https://doi.org/10.1002/art.22180
  22. Madhu K, Chanda K, Saji MJ. Safety and efficacy of Curcuma longa extract in the treatment of painful knee osteoarthritis: a randomized placebo-controlled trial. Inflammopharmacology. 2013;21(2):129-36. https://doi.org/10.1007/s10787-012-0163-3
  23. Pinsornsak P, Niempoog S. The efficacy of Curcuma Longa L. extract as an adjuvant therapy in primary knee osteoarthritis: a randomized control trial. J Med Assoc Thai. 2012;95 Suppl 1:S51-8.
  24. Combe R, Bramwell S, Field MJ. The monosodium iodoacetate model of osteoarthritis. Neurosci Lett. 2004; 370(2-3):236-40. https://doi.org/10.1016/j.neulet.2004.08.023
  25. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic change of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol. 2003;31(6):619-24. https://doi.org/10.1080/01926230390241800
  26. Bove SE, Calcaterra SL, Brooker RM, Huber CM, Guzman RE, Juneau PL, Schrier DJ, Kilgore KS. Weight bearing as a measure of disease progression and efficacy of anti- inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis. Osteoarthritis Cartilage. 2003;11(11):821-30. https://doi.org/10.1016/S1063-4584(03)00163-8
  27. Schuelert N, McDougall JJ. Grading of monosodium iodoacetate- induced osteoarthritis reveals a concentrationdependent sensitization of nociceptors in the knee joint of the rat. Neurosci Lett. 2009;465(2):184-8. https://doi.org/10.1016/j.neulet.2009.08.063
  28. Zhu CZ, Hsieh G, Ei-Kouhen O, Wilson SG, Mikusa JP, Hollingsworth PR, Chang R, Moreland RB, Brioni J, Decker MW, Honore P. Role of central and peripheral mGluR5 receptors in post-operative pain in rats. Pain. 2005;114(1-2):195-202. https://doi.org/10.1016/j.pain.2004.12.016
  29. Henrotin Y, Kurz B, Aigner T. Oxygen and reactive oxygen species in cartilage degradation: friends or foes? Osteoarthritis Cartilage. 2005;13(8):643-54. https://doi.org/10.1016/j.joca.2005.04.002
  30. Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci U S A. 1990;87(4):1620-4. https://doi.org/10.1073/pnas.87.4.1620
  31. Henrotin YE, Bruckner P, Pujol JP. The role of reactive oxygen species in homeostasis and degradation of cartilage. Osteoarthritis Cartilage. 2003;11(10):747-55. https://doi.org/10.1016/S1063-4584(03)00150-X
  32. Takac I, Schroder K, Zhang L, Lardy B, Anilkumar N, Lambeth JD, Shah AM, Morel F, Brandes RP. The E-loop is involved in hydrogen peroxide formation by the NADPH oxidase Nox4. J Biol Chem. 2011;286(15):13304-13. https://doi.org/10.1074/jbc.M110.192138
  33. Hultqvist M, Olsson LM, Gelderman KA, Holmdahl R. The protective role of ROS in autoimmune disease. Trends Immunol. 2009;30(5):201-8. https://doi.org/10.1016/j.it.2009.03.004
  34. Dang PM, Cross AR, Babior BM. Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558. Proc Natl Acad Sci U S A. 2001;98(6):3001-5. https://doi.org/10.1073/pnas.061029698
  35. Kallenborn-Gerhardt W, Schröder K, Geisslinger G, Schmidtko A. NOXious signaling in pain processing. Pharmacol Ther. 2013;137(3):309-17. https://doi.org/10.1016/j.pharmthera.2012.11.001
  36. Altenhofer S, Kleikers PW, Radermacher KA, Scheurer P, Rob Hermans JJ, Schiffers P, Ho H, Wingler K, Schmidt HH. The NOX toolbox: validating the role of NADPH oxidases in physiology and disease. Cell Mol Life Sci. 2012;69(14):2327-43. https://doi.org/10.1007/s00018-012-1010-9
  37. Jin BM, Lee MK, Lee JS, Hyun KY. Anti-inflammatory effects of Korean red ginseng extract in formalin-induced orofacial pain in rats. J Korea Acad Industr Coop Soc. 2014;15(9):5708-15. https://doi.org/10.5762/KAIS.2014.15.9.5708
  38. Lim JS, Ahn KY. Potassium depletion upregulates expression of Nrf2 transcription factor in rat kidney. Korean J Nephrol. 2011;30(3):239-45.
  39. Rotruck JT, Pope A, Ganther ME, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science. 1973; 179(4073):588-90. https://doi.org/10.1126/science.179.4073.588
  40. Preiser JC. “Oxidative stress”. JPEN J Parenter Enteral Nutr. 2012;36(2):147-54. https://doi.org/10.1177/0148607111434963
  41. Lemos FB, Ijzermans JN, Zondervan PE, Peeters AM, Van Den Engel S, Mol WM, Weimar W, Baan CC. Differential expression of heme oxygenase-1 and vascular endothelial growth factor in cadaveric and living donor kidneys after ischemia-reperfusion. J Am Soc Nephrol. 2003;14(12):3278-87. https://doi.org/10.1097/01.ASN.0000098683.92538.66
  42. Beg AA, Baldwin AS Jr. The I kappa B protein: multifunctional regulators of Rel/NF-kappa B transcription factor. Genes Dev. 1993;7(11):2064-70. https://doi.org/10.1101/gad.7.11.2064
  43. Palombella VJ, Rando OJ, Goldberg AL, Maniatis T. The ubiquitinproteasome pathway is required for processing the $NF-{\kappa}$ B1 precursor protein and the activation of NF- ${\kappa}B$. Cell. 1996;78(5):773-85.
  44. Kuprsh DV, Udalova IA, Turetskaya RL, Rice NR, Nedospasov SAC. Convserved kappa B element located downstream of the tumor necrosis factor alpha gene:distinct NF-kappa B binding pattern and enhancer activity in LPS activated murine macrophages. Oncogene. 1995;11(1):97-106.
  45. Galien R, Evans HF, Garcia T. Involvement of CCAAT/ enhancer-binding protein and nuclear factor-kappa B binding site in interleukin-6 promoter inhibition by estrogens. Mol Endocrinol. 1996;10(6):713-22. https://doi.org/10.1210/mend.10.6.8776731
  46. Brune K, Hinz B. Selective cyclooxygenase-2 inhibitors: similarities and differences. Scand J Rheumatol. 2004; 33(1):1-6. https://doi.org/10.1080/03009740310004766
  47. Richardot P, Charni-Ben Tabassi N, Toh L, Marotte H, Bay-Jensen AC, Miossec P, Garnero P. Nitrated type III collagen as a biological marker of nitric oxide-mediated synovial tissue metabolism in osteoarthritis. Osteoarthritis Cartilage. 2009;17(10):1362-7. https://doi.org/10.1016/j.joca.2009.04.024
  48. McCartney-Francis NL, Song X, Mizel DE, Wahl SM. Selective inhibition of inducible nitric oxide synthase exacerbates erosive joint disease. J Immunol. 2001;166(4): 2734-40. https://doi.org/10.4049/jimmunol.166.4.2734
  49. Iwanami K, Matsumoto I, Tanaka-Watanabe Y, Inoue A, Mihara M, Ohsugi Y, Mamura M, Goto D, Ito S, Tsutsumi A, Kishimoto T, Sumida T. Crucial role of interleukin- 6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase. Arthritis Rheum. 2008;58(3):754-63. https://doi.org/10.1002/art.23222
  50. Nelson AR, Fingleton B, Rothenberg ML, Matrisian LM. Matrix metalloproteinases: biolobic activity ans clinical implication. J Clin Oncol. 2000;18(5):1135-49. https://doi.org/10.1200/JCO.2000.18.5.1135
  51. Zeng ZS, Cohen AM, Guillem JG. Loss of basement membrane type IV collagen is associated with increased expression of metalloproteinases 2 and 9 (MMP-2 and MMP-9) during human colorectal tumorigenesis. Carcinogenesis. 1999;20(5):749-55. https://doi.org/10.1093/carcin/20.5.749
  52. Nagasa H, Woessner JF. Matrix metalloproteinases. J Biol Chem. 1999;274(31):21491-4. https://doi.org/10.1074/jbc.274.31.21491
  53. Halliwell B. Oral inflammation and reactive species: a missed opportunity. Oral Dis. 2000;6(3):137.
  54. Lambert E, Dasse E, Haye B, Petitfrere E. TIMPs as multifacial proteins. Crit Rev Oncol Hematol. 2004;49(3): 187-98. https://doi.org/10.1016/j.critrevonc.2003.09.008
  55. Dijkgraaf LC, de Bont LG, Boering G, Liem RS. Normal cartilage structure, biochemistry, and metabolism: a review of the literature. J Oral Maxillofac Surg. 1995;53(8): 924-9. https://doi.org/10.1016/0278-2391(95)90283-X
  56. Shinomura T. Proteoglycan knock-out mice and osteoarthritis. Clin Calcium. 2004;14(7):58-63.