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티트리 (Melaleuca alternifolia) 추출물의 항균 활성

Antimicrobial Effects of Tea Tree (Melaleuca alternifolia) Extracts

  • 지근호 (부경대학교 기초과학연구소)
  • Ji, Keunho (Basic Science Research Institute, Pukyong National University)
  • 투고 : 2019.02.25
  • 심사 : 2019.05.03
  • 발행 : 2019.05.31

초록

티트리 (Melaleuca alternifolia, Tea tree)는 terpineol-4, cineol, cymene, sesquiterpenes 등을 함유하여 살균 효과와 피부 보습효과를 지니고 있으며 또한, 여드름 염증 완화, 비듬 치료, 통증 완화, 우울증 해소 등의 특성을 가지고 있다. 본 연구에서는 티트리를 유기용매 (Methanol, Dichloromethane, Ethyl acetate) 및 열수추출법을 이용하여 물질을 추출하였으며, 각 추출액에서 나타나는 항균 및 항진균 활성을 확인하였다. 항균 활성 검증은 9종의 병원성 미생물 (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio parahaemolyticus)을 대상으로 실시하였으며, 항균 활성 검증에 일반적으로 사용되는 disc diffusion법을 이용하여 추출물의 항균 활성을 확인하였다. 항균활성 검증을 통하여 ethyl acetate 분획추출물과 methanol 분획추출물에서 V. parahemolyticus와, S. aureus에 대한 항균활성을 확인하였다. 또한, 최대 활성을 나타내었던 V. parahaemolyticus를 대상으로 각 분획추출물 및 열수추출물의 항균활성을 확인하였으며, 10 mg/mL 농도로 처리하였을 때 99.9 % 이상의 항균활성을 확인하였다. 항진균활성의 경우, 균종에 따른 차이는 있으나 진균류에 대한 약 45시간 이상의 보존력을 확인하였다. 본 연구를 통해서 티트리 열수추출물과 메탄올 추출물의 V. parahaemolyticus에 대한 항균 활성을 확인하였으며, 추가적인 연구를 통해 항균제 및 항진균제로써의 개발 가능성을 확인하고자 한다.

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Fig. 1. The procedure for each extraction from Melaleuca alternifolia.

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Fig. 2. The antimicrobial activities of M. alternifolia extracts. C; DMSO control, D; dichloromethane extract, E; ethyl acetate extract, H; water layer

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Fig. 3. The antimicrobial activities of M. alternifolia extracts. B; MeOH control, 1; 99 % MeOH extract, 2; 70 % MeOH extract

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Fig. 4. The antimicrobial activities of M. alternifolia extracts. B; DMSO control, 1; MeOHext- DMSOdis, 2; MeOHext-H2Odis, 3; Hot water extract

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Fig. 5. The antifungal activities of M. alternifolia extracts against A. niger . A; 24hr, B; 45hr, C; 70hr, D; 95hr, E; 145hr, (1); Control, (2); 70 % MeOH extract, (3); 99 % MeOH extract, (4); MeOHext-DMSOdis, (5); Hot water extract

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Fig. 6. The antifungal activities of M. alternifolia extracts against C . globosum . A; 24hr, B; 45hr, C; 70hr, D; 95hr, E; 145hr, (1); Control, (2); 70 % MeOH extract, (3); 99 % MeOH extract, (4); MeOHext-DMSOdis, (5); Hot water extract

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Fig. 7. The antifungal activities of M. alternifolia extracts against P. pinophilum . A; 24hr, B; 45hr, C; 70hr, D; 95hr, E; 145hr, (1); Control, (2); 70 % MeOH extract, (3); 99 % MeOH extract, (4); MeOHext-DMSOdis, (5); Hot water extract

Table 1. Growth conditions of pathogenic bacteria

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Table 2. Antimicrobial effects of M. alternifolia various extracts against V. parahaemolyticus.

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과제정보

연구 과제 주관 기관 : 한국연구재단

참고문헌

  1. J. Y. An, "Levels of Health and Subjective Life Expectancy among Community-dwelling Elders in Korea", J Kor Gerontol Nursing, Vol.20, No.1, pp.22-34, 2018. DOI: https://doi.org/10.17079/jkgn.2018.20.1.22 https://doi.org/10.17079/jkgn.2018.20.1.22
  2. J. Y. Yoon, An Analysis of the Market and Demand of Aging-Friendly Medicines. p.1-8, KHIDI, 2015.
  3. D. J. Kwon, J. H. Park, M. Kwon, J. Y. Yoo, Y. J. Koo, "Optimal Extracting Condition of Growth-inhibitory Component of Wormwood (Artemisia princeps ) against Clostridium perfringens ", J Kor Soc Agricul Chem Biotechnol, Vol.40, No.4, pp.267-270, 1997.
  4. J. H. Guk, S. J. Ma, K. H. Park, "Isolation and Characterization of Benzoic Acid with Antimicrobial Activity from Needle of Pinus densiflora ", Kor J Food Sci technol, Vol.29, No.2, pp.204-210, 1997.
  5. C. S. Park, "Antibacterial Activity of Water Extract of Green Tea against Pathogenic Bacteria", Kor J Food Preserv, Vol.5, No.3, pp.286-291, 1998.
  6. J. H. Bae, "Effect of Extracts from Paeonia japonica on the Growth of Food-borne Pathogens", J East Asian Soc Diet Life, Vol.21, No.2, pp.272-276, 2011.
  7. H. S. Choi, H. S. Song, H. Ukeda, M. Sawamura, " Radical Scavenging Activities of Citrus Essential Oils and Their Components: Detection Using 1,1-diphenyl-2-picrylhydrazyl", J Agric Food Chem, Vol.48, No.9, pp.4156-416, 2000. DOI: https://doi.org/10.1021/jf000227d https://doi.org/10.1021/jf000227d
  8. K. F. Massry, A. H. El-Ghorab, A. Farouk, " Antioxidant Activity and Volatile Components of Egyptian Artemisia judaica L", Food Chem Vol.79, No.3, pp.331-336, 2002. DOI: https://doi.org/10.1016/S0308-8146(02)00164-4 https://doi.org/10.1016/S0308-8146(02)00164-4
  9. F. Juteau, V. Masotti, J. M. Bessiere, M. Dherbomez, J. Viano, "Antibacterial and Antioxidant Activies of Artemisia annua Essential Oil", Fitoterapia , Vol.73, No.6, pp.532-535, 2002. DOI: https://doi.org/10.1016/S0367-326X(02)00175-2 https://doi.org/10.1016/S0367-326X(02)00175-2
  10. M. J. Lee, G. S. Moon, "Antioxidative Effects of Korean Bamboo Trees, Wang-dae, Som-dae, Maengjong-juk, Jolit-dae and O-juk", Kor J Food Sci Techno, Vol.35, No.6, pp.1226-1232. 2003.
  11. E. Y. Kim, I. N. Baik, J. H. Kim, S. R. Kim, M. R. Rhyu, "Screening of Antioxidant Activity of Some Medicinal Plants", Kor J Food Sci Technol , Vol.36, No.2, pp.333-338, 2004.
  12. J. A. Kim, J. M. Lee, "The Change of Biologically Functional Compounds and Antioxidant Activities in Hizikia fusiformis with Drying Methods", Kor J Food Cul, Vol.19, No.2, pp.200-208, 2004.
  13. M.M. Tong, P. M. Altman, R. S. Barnetson, "Tea Tree Oil in the Treatment of Tinea pedis ", Australas J Dermatol, Vol.33, No.3, pp.145-149, 1992. DOI: https://doi.org/10.1111/j.1440-0960.1992.tb00103.x https://doi.org/10.1111/j.1440-0960.1992.tb00103.x
  14. S. H. Kim, S. Y. Lee, L. Shunhua, "The Effect of Essential Oils from Tea-tree and Palmarosa on the Acne Skin", Kor J Aesthet Cosmetol, Vol.11, No.6, pp.1083-1090, 2013.
  15. I. K. Park, H. Koo, Y. S. Chun, "A Case of Phthiriasis Palpebrarum Treated with Tea Tree Oil in a Child", J Kor Ophthalmol Soc, Vol.52, No.10, pp.1222-1226, 2011. DOI: https://doi.org/10.3341/jkos.2011.52.10.1222 https://doi.org/10.3341/jkos.2011.52.10.1222
  16. E. H. Park, "A Study on Efficiency of Essential Oil for Dandruff Symptoms Relief-Focused on Tea Tree Oil-", J Kor Soc Fashion Beauty, Vol.3, No.3, pp.31-40, 2005.
  17. Clinical Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard- 12th Ed, M07-A10. p27-47, Wayne, PA: Clinical Laboratory Standards Institute; 2015.
  18. Merghni, Abderrahmen, et al. "Assessment of the antibiofilm and antiquorum sensing activities of Eucalyptus globulus essential oil and its main component 1, 8-cineole against methicillin-resistant Staphylococcus aureus strains" Microb pathog , Vol.118, pp.74-80, 2018. DOI: https://doi.org/10.1016/j.micpath.2018.03.006 https://doi.org/10.1016/j.micpath.2018.03.006
  19. P. S. Kwon, D-J. Kim, H. Park, "Improved Antibacterial Effect of Blending Essential Oils", Kor J Clin Lab Sci, Vol.49, No.3 pp.256-262, 2017. DOI: https://doi.org/10.15324/kjcls.2017.49.3.256 https://doi.org/10.15324/kjcls.2017.49.3.256
  20. de Sa Silva, Claudileide, et al. "Inhibition of Listeria monocytogenes by Melaleuca alternifolia (tea tree) essential oil in ground beef", Int J Food Microbiol, Vol.293, pp.79-86, 2019. DOI: https://doi.org/10.1016/j.ijfoodmicro.2019.01.004 https://doi.org/10.1016/j.ijfoodmicro.2019.01.004
  21. J. Nguefack, B. B. Budde, M. Jakobsen, "Five Essential Oils from Aromatic Plants of Cameroon: Their Antibacterial Activity and Ability to Permeabilize the Cytoplasmic Membrane of Listeria innocua Examined by Flow Cytometry", Lett App Microbiol, Vol.39, No.5, pp.395-400, 2004. DOI: https://doi.org/10.1111/j.1472-765X.2004.01587.x https://doi.org/10.1111/j.1472-765X.2004.01587.x
  22. M. J. Kim, T. K. Jung, I. G. Hong, K. S. Yoon, "Comparision of Antimicrobial Oil as Natural Preservatives", J Soc Cosmet Scientists Kor, Vol.32, No.2, pp.99-103, 2006.
  23. Yano, Yutaka, Masataka Satomi, and Hiroshi Oikawa. "Antimicrobial effect of spices and herbs on Vibrio parahaemolyticus ", Int J Food Microbiol, Vol,111, No.1 pp.6-11, 2006. DOI: https://doi.org/10.1016/j.ijfoodmicro.2006.04.031 https://doi.org/10.1016/j.ijfoodmicro.2006.04.031
  24. E-H. Kim, Y-A. Jang, S-B. Kim, H-H. Kim, J-T. Lee, "Antimicrobial, antifungal effect and safety verification using BCOP assay of extract from Coptis chinensis ", J Appl Biol Chem, Vol.61, No.3, pp.297-304, 2018. DOI: https://doi.org/10.3839/jabc.2018.042 https://doi.org/10.3839/jabc.2018.042