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대사체분석 기술을 이용한 들깨 종자의 품질 특성 및 항산화 효과

Analysis of the seed metabolite profiles and antioxidant activity of perilla varieties

  • 구수연 (경상대학교 응용생명과학부) ;
  • 손예진 (경상대학교 응용생명과학부) ;
  • 박지영 (경상대학교 응용생명과학부) ;
  • 최성길 (경상대학교 농업생명과학대학 식품공학과) ;
  • 이명희 (국립식량과학원 남부작물부) ;
  • 김현진 (경상대학교 응용생명과학부)
  • Gu, Suyeon (Division of Applied Life Sciences (BK21 plus)) ;
  • Son, Yejin (Division of Applied Life Sciences (BK21 plus)) ;
  • Park, Ji Yeong (Division of Applied Life Sciences (BK21 plus)) ;
  • Choi, Sung-Gil (Department of Food Science and Technology, and Institute of Agriculure and Life Science) ;
  • Lee, Myoung Hee (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Hyun-Jin (Division of Applied Life Sciences (BK21 plus))
  • 투고 : 2019.03.15
  • 심사 : 2019.05.02
  • 발행 : 2019.06.30

초록

지방산과 이차대사물질들의 프로파일들을 종합적으로 비교한 결과 지방산의 함량이 높은 Okdong, Deulsaem, Daesil 품종의 총 페놀화합물과 항산화 활성은 낮았으나 Joim, Yeupsil, Danjo 품종은 지방산과 총 페놀화합물 함량이 상대적으로 높은 것으로 확인되었으며 Yeupsil 품종이 그 중에서 가장 우수한 것으로 확인되었다. 따라서 기름의 함량만으로는 Okdong과 Deulsaem 품종이 우수한 반면 기름 함량과 다양한 건강기능성을 갖고 있는 것으로 확인된 rosmarinyl glucoside와 rosmarinic acid을 비롯한 기능성 이차산물의 함량도 높은 것을 고려한다면 Yeupsil 품종이 우수한 것으로 확인되어 들기름 뿐만 아니라 들깨가루용으로도 적합할 것으로 사료된다. 반면에 지방산 함량과 이차대사물질의 함량이 낮은 것으로 확인된 Dasil, Daeyeop, Saeyeupsil 품종은 식품 가공용 들깨 품종으로는 적합하지 않는 것으로 사료된다.

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Fig. 1. 29 varieties of perilla seed.

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Fig. 2. Representative chromatogram of fatty acids (A) and metabolite profiles (B) analyzed by GC-MS and by UPLC-Q-TOF MS, respectively.

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Fig. 3. PLS-DA (Partial Least Squares Discriminant Analysis) score plots of perilla seed fatty acids analyzed using gas chromatographymass spectrometry (A) and perilla seed metabolites analyzed using UPLC-Q-TOF MS (B).

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Fig. 4 Heat maps of perilla seed fatty acids (A) and perilla seed metabolites (B) contributing to the separation among samples.

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Fig. 5 Total fatty acid relative abundance (A), total phenolic content (B), and antioxidant activity (C) of 29 varieties of perilla seed.

Table 1. Identification of major metabolites contributing to the separation on the PLS-DA scores plot of the data nalyzed by GC-MS

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Table 2. Identification of major metabolites contributing to the separation on the PLS-DA scores plot of the data analyzed by LC-MS

SPGHB5_2019_v51n3_193_t0002.png 이미지

과제정보

연구 과제 주관 기관 : 농촌진흥청

참고문헌

  1. Cho JH, Song YC, Lee KS, Choi SW, Lee MJ, Jang KC, Kim HY, Kang HJ, Park KD, Seo WD. Physicochemical characteristics of cultivated aromatic rice germplasm and comparative analysis of flavor components during transplanting time. Korean J. Environ. Agric. 36: 175-183 (2017)
  2. Choung MG. Comparison of major characteristics between seed perilla and vegetable perilla. Korean J. Crop Sci. 50: 171-174 (2005)
  3. Chua YG, Bloodworth BC, Leong LP, Li SF. Metabolite profiling of edible bird's nest using gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry. Rapid Commun. Mass Spectrom. 28: 1387-1400 (2014)
  4. Gonzalo Ortiz EC, Raul SV, Omar SM, David HP, Manuel C, Maria Isabel B. In vitro antifungal activity of residues from essential oil industry against penicillium verrucosum, a common contaminant of ripening cheeses. LWT-Food Sci. Technol. 73: 226-232 (2016)
  5. Ha TJ, Lee MH, Park CH, Kim JI, Oh EY, Pae SB, Park JE, Kim SU, Kwak DY. rvH1N1 Neuraminidase inhibitory activities of phenolics from Perilla frutescens (L.) and their contents in cultivars and germplasm. Korean J. Breed. Sci. 6: 404-412 (2018)
  6. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36: 333-338 (2004)
  7. Kim SW, Koo BC, Kim JH, Liu JR. Metabolic discrimination of rice cultivars and relative quantification of major sugar compounds using H NMR spectroscopy combined by multivariate statistical analysis. J. Plant Biotechnol. 33: 283-288 (2006)
  8. Kim SW, Kwon YK, Kim JH, Liu JR. Present and prospect of plant metabolomics. J. Plant Biotechnol. 37: 12-24 (2010)
  9. Kim JK, Park SY, Na JK, Seong ES, Yu CY. Metabolite profiling based on lipophilic compounds for quality assessment of perilla (Perilla frutescens) cultivars. J. Agr. Food Chem. 60: 2257-2263 (2012)
  10. Kim SN, Song BR, Ju JH. Antioxidant activities of Perilla frutescens britton seed extract and its inhibitory effects against major characteristics of cancer cells. J. Korean Soc. Food Sci. Nutr. 44: 208-215 (2015)
  11. Lee YH, Kim BK, Kim SY, Kim MS, Kim HJ, Hwang SR, Kim K, Lee JH. Characterization of metabolite profiles from the leaves of green perilla (Perilla frutescens) by ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry and screening for their antioxidant properties. J. Food Drug Anal. 25: 776-788 (2017)
  12. Lee JH, Park KH, Lee MH, Kim HT, Seo WD, Kim JY, Baek IY, Jang DS, Ha TJ. Identification, characterization, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chem. 136: 843-852 (2013) https://doi.org/10.1016/j.foodchem.2012.08.057
  13. Lee JM, P. Rodriguez J, Kim YJ, Lee MH, Cho EJ, Lee SH. Fatty acid content in perilla cultivars and commercial oils determined by GC analysis. Nat. Prod. Sci. 22: 259-262 (2016) https://doi.org/10.20307/nps.2016.22.4.259
  14. Lee YJ, Lee KT. Identification of volatile compounds of 4 grape species by storage conditions. J. Korean Soc. Food Sci. Nutr. 36: 874-880 (2007) https://doi.org/10.3746/jkfn.2007.36.7.874
  15. Li H, Zhang Z, He D, Xia Y, Liu Q, Li X. Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity. Food Sci. Technol. Campinas. 37: 71-77 (2017)
  16. Petersen M, Simmonds MSJ. Rosmarinic acid. Phytochemistry 62: 121-125 (2003)
  17. Shin HS, Kim SW. Lipid composition of perilla seed. J. Am. Oil Chem. Soc. 71: 619-622 (1994)
  18. Singleton VL, Orthofer R, Lamuela-raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299: 152-178 (1999)
  19. Wang SY, Choe EO. Oxidative stability and antioxidant changes in perilla seeds and perilla oil affected by UV irradiation. Korean J. Food Sci. Technol. 44: 8-13 (2012)
  20. Yang SY, Kim HG, Lee SJ, Cha WM, Ahn CH, Boo HO. Comparison of the antioxidative abilities of greenhouse-grown cucumber according to cultivars and growth stages. Korean J. Plant Res. 26: 548-556 (2013)
  21. Zhao Y, Zhao J, Zhao C, Zhou H, Li Y, Zhang J, Li L, Hu C, Li W, Peng X, Lu X, Lin F, Xu G. A metabolomics study delineating geographical location-associated primary metabolic changes in the leaves of growing tobacco plants by GC-MS and CE-MS. Sci. Rep. 2015: 16346 (2015)