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Development of an apple/pear pomace fermented with Lentinus edodes Mycelia

사과/배 부산물 및 표고버섯균사체를 이용한 발효물 개발

  • 김진경 ((재)발효미생물산업진흥원) ;
  • 조승화 ((재)발효미생물산업진흥원) ;
  • 김은지 ((재)발효미생물산업진흥원) ;
  • 정도연 ((재)발효미생물산업진흥원)
  • Received : 2019.01.24
  • Accepted : 2019.04.11
  • Published : 2019.06.30

Abstract

The purpose of this study was to investigate the possibility of enhancing the functional compounds in apple and pear pomace (APP) by fermentation with mycelia from the mushroom Lentinula edodes. A 30% (w/v) APP with added rice bran and Biji was fermented with L. edodes at $24^{\circ}C$ and 80% humidity. The cellulase and pectinase activities in the fermented APP (FAPP) were higher than those in the non-fermented control. In addition, the physiological activities of the FAPP, including DPPH, ABTS radical scavenging, and SOD-like activity, as well as the total polyphenol and ${\beta}-glucan$ contents were higher than those in the control. FAPP treatment significantly reduced LPS-induced nitric oxide (NO) levels in Raw 264.7 cell. Therefore, FAPP treatment was considered to more effectively suppress cell injury caused by inflammatory cytokines through inhibition of LPS-induced NO production. These results suggest that the levels of functional components in APP can be increased by fermentation with this mushroom mycelium. However, further studies are needed before it can be used as a functional material.

Keywords

apple/pear pomace;DPPH radical scavenging;ABTS radical;SOD-like activity;${\beta}-glucan$

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Fig. 1. Total phenolic contents of apple/pear pomace ferment with Lentinula edodes mycelia.

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Fig. 2. DPPH radical scavenging of apple/pear pomace ferment with Lentinula edodes mycelia.

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Fig. 3. SOD-like activity of apple/pear pomace ferment with Lentinula edodes mycelia.

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Fig. 4. ABTS+ cation radical scavengign activity of apple/pear pomace ferment with Lentinula edodes mycelia.

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Fig. 5. Effect of apple/pear pomace ferment with Lentinula edodes mycelia on the cell viability of raw 264.7 cell.

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Fig. 6. Effect of apple/pear pomace ferment with Lentinula edodes mycelia on the cell viability of raw 264.7 cell.

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Fig. 7. Production of nitric oxide in apple/pear pomace ferment with Lentinula edodes mycelia.

Table 1. Different addition ratio of nutrient for apple/pear pomace ferment with Lentinula edodes mycelia

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Table 2. Proximate of apple/pear pomace ferment with Lentinula edodes mycelia

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Table 3. Enzyme activities of apple/pear pomace ferment with Lentinula edodes mycelia

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Table 4. β-glucan contents of apple/pear pomace ferment with Lentinula edodes mycelia

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