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옥돔과 옥두어 판별을 위한 PCR 검사법 개발과 검증

Development and validation of a PCR method to discriminate between Branchiostegus japonicus and Branchiostegus albus

  • Kim, Na-Ye-Seul (Department of Food science and Technology, Suncheon National University) ;
  • Yang, Ji-Young (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Jung-Beom (Department of Food science and Technology, Suncheon National University)
  • 투고 : 2019.03.04
  • 심사 : 2019.04.12
  • 발행 : 2019.06.30

초록

본 연구에서는 형태학적으로 판별이 어려운 옥돔과 옥두어의 종 특이 primer를 개발, 검증한 후 모니터링을 통해 위변조 된 옥돔의 유통을 예방하고자 하였다. 옥돔과 옥두어 염기서열은 clustal omega 프로그램을 이용하여 정리하였고, primer3 프로그램을 이용하여 primer를 설계하였다. Multiplex PCR 결과는 옥돔과 옥두어에 대한 종 특이적 증폭이 확인되었고, PCR 반응을 확인하기 위한 공통 유전자에 대한 증폭이 확인되었다. 옥돔을 288 bp, 옥두어를 159 bp, 공통 유전자를 502 bp로 증폭되어 각각 PCR product 사이에 100 bp 이상 차이가 나타나 정확하게 판별이 가능하였다. Multiplex PCR 민감도 실험결과 옥돔 primer가 1 ng, 옥두어 primer가 1 ng, 공통 유전자 primer가 1 ng까지 밴드가 확인되었다. 모니터링 실험결과, 옥돔 38건, 옥두어 13건으로 판정되어 시료의 어종과 실험결과가 100% 일치함을 확인하였고, 위변조 사례는 나타나지 않았다. 본 실험에서 개발된 multiplex PCR 방법은 특이도와 민감도가 확보되었고 모니터링을 통해 유통, 판매되고 있는 옥돔과 옥두어의 판별에 적합함을 확인하였다.

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Fig. 1. Multiplex PCR specificity of B. japonicus and B. albus.

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Fig. 2. Multiplex PCR sensitivity of B. japonicus and B. albus.

Table 1. Primer sequence designed in this study

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Table 2. Monitoring results of B. japonicus and B. albus on the market

SPGHB5_2019_v51n3_295_t0002.png 이미지

과제정보

연구 과제 주관 기관 : 식품의약품안전처

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