DOI QR코드

DOI QR Code

Liver Dysfunction and Oxidative Stress in Streptozotocin-Induced Diabetic Rats: Protective Role of Artemisia Turanica

  • Yazdi, Hassan Bgheri ;
  • Hojati, Vida ;
  • Shiravi, Abdolhossein ;
  • Hosseinian, Sara ;
  • Vaezi, Gholamhassan ;
  • Hadjzadeh, Mousa-Al-Reza
  • Received : 2018.08.27
  • Accepted : 2019.05.20
  • Published : 2019.06.28

Abstract

Objectives: Oxidative stress plays a central role in diabetes-induced complications. In the present study, the protevtive effect of Artemisia turanica (A. turanica) was evaluated against diabetes-induced liver oxidative stress and dysfunction. Methods: Fifty male Wistar rats were randomly divided into five groups: control, diabetic, diabetic + metformin, diabetic + A. turanica extract, and diabetic + A. turanica extract + metformin. Experimental diabetes was induced by a single-dose (55 mg/kg, intraperitoneally (ip)) injection of streptozotocin (STZ). Metformin (300 mg/kg) and A. turanica extract (70 mg/kg) were orally administrated three days after STZ injection for four weeks. The levels of malondialdehyde (MDA), total thiol content and superoxide dismutase (SOD) and catalase activities were measured in the liver tissue. Serum glucose concentration, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were also determined. Results: In the diabetic group, serum glucose concentration, serum AST and ALT activities and liver MDA level were significantly higher while tissue total thiol content as well as catalase and SOD activities were lower, compared to the control group. Serum glucose in diabetic rats treated with metformin + A. turanica extract showed a significant decrease compared with the diabetic group. In all the A. turanica extract and metformin treated groups, serum ALT, tissue MDA level, total thiol content and SOD activity significantly improved compared with the diabetic rats. However, treatment of the diabetic rats only with metformin could not significantly change the activities of catalase and AST compared with the diabetic group. Conclusion: These findings suggested that A. turanica extract had a therapeutic effect on liver dysfuncyion and oxidative stress induced by diabetes, that may be probably due to its antioxidant and antiinflammatory effects.

Keywords

diabetes mellitus;artemisia turanica;metformin;oxidative stress

DHOCBS_2019_v22n2_109_f0001.png 이미지

Figure 1 Glucose concentration in all experimental groups. Values are the Mean±SEM. The data were analyzed using one-way ANOVA & post hoc LSD.

DHOCBS_2019_v22n2_109_f0001.png 이미지

Figure 1 Glucose concentration in all experimental groups. Values are the Mean±SEM. The data were analyzed using one-way ANOVA & post hoc LSD.

DHOCBS_2019_v22n2_109_f0002.png 이미지

Figure 2 Liver MDA concentration in all experimental groups. Values are the Mean±SEM. The data were analyzed using oneway ANOVA & post hoc LSD.

DHOCBS_2019_v22n2_109_f0002.png 이미지

Figure 2 Liver MDA concentration in all experimental groups. Values are the Mean±SEM. The data were analyzed using oneway ANOVA & post hoc LSD.

Table 1 The effect of A. turanica on serum AST and ALT activities in all experimental groups.

DHOCBS_2019_v22n2_109_t0001.png 이미지

Table 1 The effect of A. turanica on serum AST and ALT activities in all experimental groups.

DHOCBS_2019_v22n2_109_t0001.png 이미지

References

  1. Ruch W, Zumsteg U. Diabetes or hyperglycemia? Schweizerische medizinische Wochenschrift. 1988;118(8):264-266.
  2. Amos AF, McCarty DJ, Zimmet P. The rising global burden of diabetes and its complications: estimates and projections to the year 2010. Diabetic medicine. 1997;14(S5).
  3. Levinthal GN, Tavill AS. Liver disease and diabetes mellitus. Clinical diabetes. 1999;17(2):73.
  4. Ahmad FK, He Z, King GL. Molecular targets of diabetic cardiovascular complications. Current drug targets. 2005;6(4):487-494. https://doi.org/10.2174/1389450054021990
  5. Jiang Z-Y, Woollard A, Wolff SP. Hydrogen peroxide production during experimental protein glycation. Febs Letters. 1990;268(1):69-71. https://doi.org/10.1016/0014-5793(90)80974-N
  6. Mohamed J, Nafizah AN, Zariyantey A, Budin SB. Mechanisms of Diabetes-Induced Liver Damage: The role of oxidative stress and inflammation. Sultan Qaboos University Medical Journal. 2016;16(2):e132. https://doi.org/10.18295/squmj.2016.16.02.002
  7. Balasubramanian T, Senthilkumar G, Karthikeyan M, Chatterjee TK. Protective effect of ethyl acetate fraction of stereospermum suaveolens against hepatic oxidative stress in STZ diabetic rats. Journal of traditional and complementary medicine. 2013;3(3):175-181. https://doi.org/10.4103/2225-4110.114904
  8. Hassani FV, Mehri S, Abnous K, Birner-Gruenberger R, Hosseinzadeh H. Protective effect of crocin on BPA-induced liver toxicity in rats through inhibition of oxidative stress and downregulation of MAPK and MAPKAP signaling pathway and miRNA-122 expression. Food and Chemical Toxicology. 2017;107:395-405. https://doi.org/10.1016/j.fct.2017.07.007
  9. Alam MM, Meerza D, Naseem I. Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice. Life sciences. 2014;109(1):8-14. https://doi.org/10.1016/j.lfs.2014.06.005
  10. Bora KS, Sharma A. The genus Artemisia: a comprehensive review. Pharmaceutical Biology. 2011;49(1):101-109. https://doi.org/10.3109/13880209.2010.497815
  11. V M. A Dictionary of Iranian Plant Names. Tehran: Farhang Moaser; 1998.
  12. Khayyat MH, Karimi H. Composition of the volatile oils of three different species of Artemisia. Iranian Journal of Pharmaceutical Sciences. 2005;1(1):33-37.
  13. Behravan J, Ramezani M, Hassanzadeh M, Eliaspour N, Sabeti Z. Cytotoxic and Antimycotic Activities of essential oil of Artemisia turanica Krasch from Iran. Journal of Essential Oil Bearing Plants. 2006;9(2):196-203. https://doi.org/10.1080/0972060X.2006.10643492
  14. Taherkhani M, Rustaiyan A, Nahrevanian H, Naeimi S, Taherkhani T. Comparison of antimalarial activity of Artemisia turanica extract with current drugs in vivo. Journal of vector borne diseases. 2013;50(1):51.
  15. Khodabandehloo F, Hosseini M, Rajaei Z, Soukhtanloo M, Farrokhi E, Rezaeipour M. Brain tissue oxidative damage as a possible mechanism for the deleterious effect of a chronic high dose of estradiol on learning and memory in ovariectomized rats. Arquivos de Neuropsiquiatria. 2013;71(5):313-319. https://doi.org/10.1590/0004-282X20130027
  16. Ellman GL. Tissue sulfhydryl groups. Archives of biochemistry and biophysics. 1959;82(1):70-77. https://doi.org/10.1016/0003-9861(59)90090-6
  17. Madesh M, Balasubramanian KA. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys. 1998;35(3):184-188. PubMed PMID: 9803669. Epub 1998/11/06. eng.
  18. Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circulation research. 2010;107(9):1058-1070. https://doi.org/10.1161/CIRCRESAHA.110.223545
  19. Ahmadieh H, Azar ST. Liver disease and diabetes: association, pathophysiology, and management. Diabetes research and clinical practice. 2014;104(1):53-62. https://doi.org/10.1016/j.diabres.2014.01.003
  20. Ghazanfar K, Ganai BA, Akbar S, Mubashir K, Dar SA, Dar MY, et al. Antidiabetic activity of Artemisia amygdalina Decne in streptozotocin induced diabetic rats. BioMed research international. 2014;2014:Article number 185676.
  21. Helal EG, Aouf NA, Khattab AM, Zoair MA. ANTI-DIABETIC EFFECT OF ARTEMISIA ANNUA (KAYSOM) IN ALLOXAN-INDUCED DIABETIC RATS. The Egyptian Journal of Hospital Medicine. 2014;57:422-430. https://doi.org/10.12816/0008476
  22. Al-Waili NSD. TREATMENT OF DIABETES MELLITUS BY ARTEMISIA HERBA-ALBA EXTRACT: PRELIMINARY STUDY. Clinical and Experimental Pharmacology and Physiology. 1986;13(7):569-574. https://doi.org/10.1111/j.1440-1681.1986.tb00940.x
  23. Nofal SM, Mahmoud SS, Ramadan A, Soliman G, Fawzy R. Anti-diabetic effect of Artemisia judaica extracts. Research Journal of Medicine and Medical Sciences. 2009;4(1):42-48.
  24. Nazaruk J, Borzym-Kluczyk M. The role of triterpenes in the management of diabetes mellitus and its complications. Phytochemistry Reviews. 2015;14(4):675-690. https://doi.org/10.1007/s11101-014-9369-x
  25. Stainsloss I, Sankarann M. Chemotherapeutic effect of 3, 3'-Diindolylmethane encapsulated chitosan nanoparticles on 7, 12-Dimethylbenz (a) anthracene induced mammary cancer - A dose dependent study. New Horizons in Translational Medicine. 2016;3(1):1-8 https://doi.org/10.1016/j.nhtm.2016.04.001
  26. Han K-H, Jeon Y-J, Athukorala Y, Choi K-D, Kim C-J, Cho J-K, et al. A water extract of Artemisia capillaris prevents 2, 2'-azobis (2-amidinopropane) dihydrochloride-induced liver damage in rats. Journal of medicinal food. 2006;9(3):342-347. https://doi.org/10.1089/jmf.2006.9.342
  27. Kim MH, Seo JY, Liu KH, Kim J-S. Protective effect of Artemisia annua L. extract against galactose-induced oxidative stress in mice. PloS one. 2014;9(7):e101486. https://doi.org/10.1371/journal.pone.0101486
  28. Sefi M, Bouaziz H, Soudani N, Boudawara T, Zeghal N. Fenthion induced-oxidative stress in the liver of adult rats and their progeny: Alleviation by Artemisia campestris. Pesticide biochemistry and physiology. 2011;101(2):71-79. https://doi.org/10.1016/j.pestbp.2011.08.002
  29. Grassmann J. Terpenoids as plant antioxidants. Vitamins & Hormones. 2005;72:505-535.
  30. Sunmonu TO, Afolayan AJ. Evaluation of antidiabetic activity and associated toxicity of Artemisia afra aqueous extract in wistar rats. Evidence-Based Complementary and Alternative Medicine. 2013;2013:Article number 929074.