DOI QR코드

DOI QR Code

Antidiabetic Activity of an Ayurvedic Formulation Chaturmukha Rasa: A Mechanism Based Study

  • Sharma, Akansha ;
  • Tiwari, Raj K ;
  • Sharma, Vikas ;
  • Pandey, Ravindra K ;
  • Shukla, Shiv Shnakar
  • Received : 2019.01.09
  • Accepted : 2019.05.20
  • Published : 2019.06.28

Abstract

Objectives: The objective of this study was to evaluate antidiabetic activity of Chaturmukha rasa based on streptozotocin induced diabetes model, alpha amylase inhibitory activity, alpha Glucosidase inhibitory activity and inhibition of sucrase. Methods: Chaturmukha rasa was prepared as per Ayurvedic formulary. Antidiabetic activity was measured in experimentally streptozotocin induced rats. The dose was taken as 45 mg/kg, i.p. The antidiabetic activity of Chaturmukha rasa was compared Triphala Kwatha, a marketed formulation. Further In vitro $\acute{\alpha}$- Amylase Inhibitory Assay, In vitro salivary amylase Inhibitory Assay, In vitro ${\alpha}-Glucosidase$ Inhibitory Assay and In vitro Sucrase Inhibitory Assay was performed with respect to Chaturmukha rasa. The IC50 value was calculated for all the above activity. Results: Streptozotocin with Acarbose showed significant decrease in blood glucose level whereas streptozotocin with Triphala kwatha showed more decrease in blood glucose level than Streptozotocin with Acarbose. The combination of Streptozotocin + Triphala kwatha + Chaturmukha rasa showed a significant decrease in blood glucose level on 21st day. In vitro $\acute{\alpha}$- Amylase Inhibitory Assay the Chaturmukha rasa showed IC50 value $495.94{\mu}l$ when compared with Acarbose $427.33{\mu}l$, respectively. In the ${\alpha}-Glucosidase$ Inhibitory Assay Chaturmukha rasa showed IC50 value $70.93{\mu}l$ when compared with Acarbose $102.28{\mu}l$, respectively. In vitro Sucrase Inhibitory Assay Chaturmukha rasa showed IC50 value $415.4{\mu}l$ when compared with Acarbose $371.43{\mu}l$, respectively. Conclusion: This study supports that Chaturmukha rasa may inhibit diabetes by inhibition of salivary amylase or alpha Glucosidase or sucrase. This may be the mechanism by which Chaturmukha rasa inhibits diabetes. Further this study supports the usage of Chaturmukha rasa for the management of diabetes.

Keywords

acarbose;$\acute{\alpha}$- Amylase Inhibitory Assay;chaturmukha rasa;IC50;${\alpha}-Glucosidase$ Inhibitory Assay;streptozotocin

DHOCBS_2019_v22n2_115_f0001.png 이미지

Figure 1 antidiabetic study of ayurvedic formulation “chaturmukha rasa” on albino wistar rat

DHOCBS_2019_v22n2_115_f0001.png 이미지

Figure 1 antidiabetic study of ayurvedic formulation “chaturmukha rasa” on albino wistar rat

DHOCBS_2019_v22n2_115_f0002.png 이미지

Figure 2 Te percent inhibition of salivary alpha-Amylase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_f0002.png 이미지

Figure 2 Te percent inhibition of salivary alpha-Amylase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_f0003.png 이미지

Figure 3 Te percent inhibition of alpha-Glucosidase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_f0003.png 이미지

Figure 3 Te percent inhibition of alpha-Glucosidase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_f0004.png 이미지

Figure 4 Te percent inhibition of sucrose by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_f0004.png 이미지

Figure 4 Te percent inhibition of sucrose by Chaturmukha rasa at varying concentrations

Table 1 Ingredients of Ayurvedic Formulation

DHOCBS_2019_v22n2_115_t0001.png 이미지

Table 1 Ingredients of Ayurvedic Formulation

DHOCBS_2019_v22n2_115_t0001.png 이미지

Table2 Dose level for different group for evaluation of toxicity study

DHOCBS_2019_v22n2_115_t0002.png 이미지

Table2 Dose level for different group for evaluation of toxicity study

DHOCBS_2019_v22n2_115_t0002.png 이미지

Table 3 antidiabetic study antidiabetic formulation “Chaturmukha rasa” on albino wistar rat.

DHOCBS_2019_v22n2_115_t0003.png 이미지

Table 3 antidiabetic study antidiabetic formulation “Chaturmukha rasa” on albino wistar rat.

DHOCBS_2019_v22n2_115_t0003.png 이미지

Table 4 The percent inhibition of salivary alpha-Amylase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_t0004.png 이미지

Table 4 The percent inhibition of salivary alpha-Amylase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_t0004.png 이미지

Table 5 The percent inhibition of alpha-Glucosidase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_t0005.png 이미지

Table 5 The percent inhibition of alpha-Glucosidase by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_t0005.png 이미지

Table 6 The percent inhi ition of sucrose by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_t0006.png 이미지

Table 6 The percent inhi ition of sucrose by Chaturmukha rasa at varying concentrations

DHOCBS_2019_v22n2_115_t0006.png 이미지

References

  1. Rajput D, Patgiri BJ, Galib RP, Prajapati PK. Anti-diabetic formulations of Naga bhasma (lead calx): A brief review. Anc Sci Life. 2013;33(1):52-59. https://doi.org/10.4103/0257-7941.134609
  2. Pandey RK, Sharma V, Tiwari RK, Shukla SS. Diabetic Foot Ulcer; Management and Novel Treatment Approaches. Indo American Journal of Pharmaceutical Sciences. 2018;5(4):2824-2828.
  3. Kazeem MI, Ogungbe SM, Saibu GM, Aboyade OM. In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts. Bangladesh J Pharmacol. 2014;9:140-145.
  4. Dimitriadis GD, Tessari P, Go VL, Gerich JE. Alpha-Glucosidase inhibition improves postprandial hyperglycemia and decreases insulin requirements in insulin-dependent diabetes mellitus. Metabolism. 1985;34(3):261-265. https://doi.org/10.1016/0026-0495(85)90010-1
  5. Pandey RK, Tiwari RK, Shukla SS. Omics: A Newer Technique in Herbal Drug Standardization and Quantification. J Young Pharm. 2016;8(2):76-81. https://doi.org/10.5530/jyp.2016.2.4
  6. Petrovska BB. Historical review of medicinal plants usage. Pharmacogn Rev.2012; 6(11): 1-5. https://doi.org/10.4103/0973-7847.95849
  7. Chaturmukha Rasa - Benefits, Dosage, Ingredients, Side Effects [Internet]. Milton Keynes: College of Ayurveda; 2012 [cites 2012 Jul 10]. Available from: https://ayurmedinfo.com/2012/07/10/chaturmukha-rasa-benefits-dosage-ingredients-side- effects/.
  8. Sahu M, Rao SP. In-vitro Inhibitory Activity of Medicinal Plant (Syzygium cumini Seed) against Carbohydrate Metabolizing Enzymes. Research journal of Pharmacognosy and Phytochemistry. 2018;10(4):55-60.
  9. Dewangan H, Tiwari RK, Sharma V, Shukla SS, Satapathy T, Pandey R. Past and Future of in-vitro and in-vivo Animal Models for Diabetes: A Review. Indian Journal of Pharmaceutical Education and Research. 2017;51(4S):s522-s530. https://doi.org/10.5530/ijper.51.4s.79
  10. Huang YN, et al. Intestinal Alpha - glucosidase inhibitory activity and toxicological evaluation of Nymphaea stellata flowers extract. Journal of Ethanopharmacology. 2010;131(2):306-3012 https://doi.org/10.1016/j.jep.2010.06.035
  11. Ahmed F, Chandra JNNS, Timmaiah NV. An In vitro study on inhibitory activities of Eugenia jambolana seeds against carbohydrate hydrolyzing enzymes. J Young Pharma. 2009;1(4):317-321. https://doi.org/10.4103/0975-1483.59320