DOI QR코드

DOI QR Code

Characteristics of the gene resources and selected strains of Agrocybe cylindracea

버들송이버섯(Agrocybe cylindracea)유전자원 및 선발계통의 특성

  • Heo, Byong-Soo (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Yoo, Young-Jin (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Seo, Sang-Young (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Kyu-Hwan (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Young Min (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kwon, Seog-Ju (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Jang, Kab-Yeul (Mushroom Research Division, NIHHS, RDA)
  • 허병수 (전라북도농업기술원) ;
  • 유영진 (전라북도농업기술원) ;
  • 서상영 (전라북도농업기술원) ;
  • 최규환 (전라북도농업기술원) ;
  • 최영민 (전라북도농업기술원) ;
  • 권석주 (전라북도농업기술원) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2019.03.07
  • Accepted : 2019.06.18
  • Published : 2019.06.30

Abstract

Agrocybe spp. belongs to the Agaricales order, Bolbitiaceae family, and Agrocybe genus. In Korea, so far, it has been cultivated through bottle cultivation; therefore, this study was conducted for the development of a new cultivar using the bag cultivation method for quantitative improvement. Thirty-three gene resources of Agrocybe spp. were collected and their quantity and characteristics of bag cultivation were examined. Next, 5 kinds of crossing parents were selected based on the cultivation period and shape of the fruit body. Seven strains were selected through 3 cross combinations. The 7 selected strains and the comparative cultivar 'Cham' were cultivated in a bag. As a result, the cultivation period was 49 days for 'JBAC15-1' and 50 days for 'JBAC15-6' which are 4 days and 3 days less than the cultivation period of the comparative cultivar 'Cham'(53 days), respectively. Cultivation periods of other strains except for 'JBAC15-1' and 'JBAC15-1' were longer than that of the comparative cultivar'Cham'. The best ratio of primordia formation among the selected strains was found to be that of 'JBAC15-1' with 96.1% followed by 'JBAC15-6' with 94.5%. These rates were 3.1% and 1.5% higher than the ratio of primordia formation of the comparative cultivar 'Cham', which is found to be 93.0%. The quantity was maximum in the 'JBAC15-1' cultivar with 176.8 g per bag followed by 'JBAC15-6' with 168.7 g per bag. The quantities were 10% and 5% more than the comparative cultivar 'Cham' with 160.7 g per bag. Based on these results, 'JBAC15-1' and 'JBAC15-6', which had shorter cultivation periods and more quantities than the comparative cultivar 'Cham' were finally selected. For the selected strains of 'JBAC15-1' and 'JBAC15-6', mycelial growth was observed to be optimal on PDA medium and the optimum temperature was $27.5^{\circ}C$. The optimum pH was pH 5 for 'JBAC15-1' and pH 6 for 'JBAC15-6'. The color of the pileus of the fruit body was dark brown in 'JBAC15-1' and 'Cham' and light brown in 'JBAC15-6'. The pileus was hemispherical in shape in both 'JBAC15-1' and 'Cham'. However, the colors of the stem were different - light brown in 'JBAC15-1', white in 'JBAC15-6', and ivory in the comparative cultivar 'Cham'.

Keywords

Agrocybe cylindracea;Cultivar;Fruit-body;Strain

BSHGBD_2019_v17n2_52_f0001.png 이미지

Fig. 2. Random amplified polymorphic DNA patterns by primer OPA-04, OPA-08, OPA-10, OPA-12. M : Marker, 1 :Beodeulsongi1ho, 2 : Sanggang, 3 : Mihwang, 4: Cham, 5 : Jin, 6 : JBAC15-1, 7 : JBAC15-6, N : Blank.

BSHGBD_2019_v17n2_52_f0002.png 이미지

Fig. 3. Photos of fruit body of A. cylindracea cultivar ‘Cham’, ‘JBAC15-1’, and ‘JBAC15-6’

BSHGBD_2019_v17n2_52_f0003.png 이미지

Fig. 1. Photos of somatic incompatibility in vitro between 5 cultivars (‘Beodeulsongi1ho’, ‘Cham’, ‘Jin’, ‘Mihwang’ and ‘Sanggang’) and ‘JBAC15-6’ of Agrocybe cylindracea.

Table 1. Cultural characteristics and quantity of collected gene resources of Agrocybe spp.

BSHGBD_2019_v17n2_52_t0001.png 이미지

Table 1. Continued

BSHGBD_2019_v17n2_52_t0002.png 이미지

Table 1. Continued

BSHGBD_2019_v17n2_52_t0003.png 이미지

Table 1. Continued

BSHGBD_2019_v17n2_52_t0004.png 이미지

Table 1. Continued

BSHGBD_2019_v17n2_52_t0005.png 이미지

Table 2. Quality characteristics of fruit body over a two year period of selected gene resources of Agrocybe cylindracea

BSHGBD_2019_v17n2_52_t0006.png 이미지

Table 3. Characteristics of mycelial growth of selected gene resources of Agrocybe cylindracea

BSHGBD_2019_v17n2_52_t0007.png 이미지

Table 4. Monosporus crossbreeding using determination of antagonism

BSHGBD_2019_v17n2_52_t0008.png 이미지

Table 5. Characteristics of mycelial growth in 35 crossbreeding strains in PDA at 25oC after 10 days

BSHGBD_2019_v17n2_52_t0009.png 이미지

Table 6. Characteristics of sawdust bag cultivation on superior strains of Agrocybe cylindracea

BSHGBD_2019_v17n2_52_t0010.png 이미지

Table 7. Characteristics of fruit body on superior strains of Agrocybe cylindracea

BSHGBD_2019_v17n2_52_t0011.png 이미지

Table 8. Characteristics of fruit body on selected strains of Agrocybe cylindracea

BSHGBD_2019_v17n2_52_t0012.png 이미지

Table 9. Effect of various media on mycelial growth of selected strains of Agrocybe cylindracea in 25oC

BSHGBD_2019_v17n2_52_t0013.png 이미지

Table 10. Effect of temperature on mycelial growth of selected strains of Agrocybe cylindracea in PDA

BSHGBD_2019_v17n2_52_t0014.png 이미지

Table 11. Effect of the pH on mycelial growth of selected strains of Agrocybe cylindracea in PDA at 25oC

BSHGBD_2019_v17n2_52_t0015.png 이미지

Acknowledgement

Supported by : 농촌진흥청

References

  1. Cha WS, Lee MY, Cho BS, Park SY, Oh DG. 2004. A study on the mycelial growth of Agrocybe aegerita in flask culture. Kor J Life Science 14: 560-566.
  2. Cheong JC, Seok DK, Kim SH, Jhune CS, Lee CJ. 2008. Characteristics of mycelial growth and fruit body production in two strains of the genus Agrocybe ASI19003(A. cylindracea) and ASI19016(A. chaxingu). J Mushroom 6: 13-19.
  3. Esser K, Semerdzieva M, Stahl U. 1974. Genetische untershchungen an dem basidiomycetes Agrocybe aegerita. Theoretical and Applied Genetics. 45:77-85. https://doi.org/10.1007/BF00283480
  4. Esser K, Meinhardt F. 1977. A common genetic control of dikaryotic and monokaryotic fruiting in the basidiomycetes Agrocybe aegerita. Molec Gen Genet 115: 113-115.
  5. Jeon DH, Choi JI, Ha T, Ju YC. 2010. Characterization of a new cultivar, 'Cham' in Agrocybe aegerita. Korean J Breed Sci 42:288-293.
  6. Kito T, Sobue S, Ukai S. 1994. Structural features and hypoglycemic activities of two polysaccharides from a hotwater extract of Agrocybe cylindracea. Carbohyd Res 251: 81-87. https://doi.org/10.1016/0008-6215(94)84277-9
  7. Labarere J, Noel T, Imbernon M. 1989. Selection and genetic analysis of antibiotic resistant mutant strains in Agrocybe aegerita. Mushroom Sci 12:161-174.
  8. Lee JY. 1988. Coloured Korean Mushroom. I. Academic Press. 137.
  9. Meinhardt F, Esser K. 1981. Genetic studies of the basidiomycetes Agrocybe aegerita. Part II genetic control of fruit body formation and its practical implications. Theoretical and Applied Genetics 60: 265-268. https://doi.org/10.1007/BF00263714
  10. Noel T, Labarere J. 1987. Isolation and regeneration of protoplasts from homokaryotic mycelia of Agrocybe aegerita. ISMS XII. p. 109.
  11. Seo SY, Ahn MS, Choi SR, Song EJ, Choi MK, Kim YS. 2011. Analysis of nutritional compositions and biological activity of Agrocybe aegerita. J Mushroom Sci 9: 166-122.