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Phosphate solubilizing effect by two paraburkholderia bacteria Isolated from button mushroom medium

양송이배지로부터 분리한 두 Paraburkholderia 속 세균에 의한 인산가용화 효과

  • Yu, Hye-Jin (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • 유혜진 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 윤민호 (충남대학교 농업생명과학대학 생물환경화학과)
  • Received : 2019.05.28
  • Accepted : 2019.06.20
  • Published : 2019.06.30

Abstract

The present study was conducted to investigate the synergistic effects caused by single and co-inoculation of the phosphate solubilizing bacteria (PSB), Paraburkholderia phenazinium YH3 and Paraburkholderia metrosideri YH4. Phosphate solubilization was assessed by measuring the phosphorus contents for 7 days in a single and co-inoculation medium. Co-inoculation of the two strains was found to release the highest content of soluble phosphorus ($1,250{\mu}g\;mL^{-1}$) into the medium, followed by the single inoculation of P. metrosideri YH4 ($1196.59{\mu}g\;mL^{-1}$) and P. phenazinium YH3 ($994.34{\mu}g\;mL^{-1}$). The highest pH reduction, organic acid production and glucose consumption was also observed in the co-inoculation medium of the two strains. A plant growth promotion bioassay revealed that co-inoculation with the two strains enhanced the growth of romaine lettuce more than single inoculation with either of the two strains (28.5% for leaf and 16.6% for root). Although there was no significant difference between single and co-inoculation of bacterial strains in terms of phosphorous release and plant growth, the synergistic effects of co-inoculation with PSB could be beneficial for crop growth.

Keywords

Lettuce Growth;Paraburkholderia metrosideri YH4;Paraburkholderia phenazinium YH3;Phosphate Solubilization

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Fig. 1. Phylogenetic tree based on 16S rDNA gene sequences, showing the position of isolate phosphate solubilizing bacterial strains YH3 (PSB-C) with respect to related species. The scale bar dindicates 0.005 substitutions per nucleotide position and accession numbers are given in parenthesis.

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Fig. 2. Phylogenetic tree based on 16S rDNA gene sequences, showing the position of isolate phosphate solubilizing bacterial strains YH4 (PSB-D) with respect to related species. The scale bar dindicates 0.005 substitutions per nucleotide position and accession numbers are given in parenthesis.

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Fig. 3. Comparison of phosphate solubilization between single and co-inoculation of P. phenazinium and P. metrosideri. Values given here are the means of three replicates (n=3).

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Fig. 4. Comparison of pH change during single and coinoculation of P. phenazinium and P. metrosideri. Values given here are the means of three replicates (n=3).

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Fig. 5. Comparison of residual glucose in the medium between single and co-inoculation of P. phenazinium and P. metrosideri. Values given here are the means of three replicates (n=3)

Table 1. HPLC analysis of organic acid by single and co-inoculation of (Paraburkholderia phenazinium and Paraburkholderia metrosideri) Values given here are the means of three replicates (n=3). ND = Not dectected.

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Table 2. Comparison on growth of romaine lettuce after 4 weeks inoculation with Paraburkholderia phenazinium and Paraburkholderia metrosideri. Values given here are the means of three replicates (n=3).

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