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양송이배지로부터 분리한 두 Paraburkholderia 속 세균에 의한 인산가용화 효과

Phosphate solubilizing effect by two paraburkholderia bacteria Isolated from button mushroom medium

  • 유혜진 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 윤민호 (충남대학교 농업생명과학대학 생물환경화학과)
  • Yu, Hye-Jin (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • 투고 : 2019.05.28
  • 심사 : 2019.06.20
  • 발행 : 2019.06.30

초록

양송이배지로부터 분리한 인산가용화균 Paraburkholderia phenazinium YH3와 Paraburkholderia metrosideri YH4의 단일접종 및 동시접종에 따른 인산가용화 능력의 시너지효과 및 상추생육효과를 조사하였다. 인산캄슘이 함유된 NBRIP 액체배지에 분리균을 접종하고 해리된 가용인산(soluble phosphorus)함량을 HPLC에 의해 분석하여 인산가용화능을 측정한 결과, 배양 3일차에 동시접종구 $1,250{\mu}g\;mL^{-1}$, P. metrosideri $1196.59{\mu}g\;mL^{-1}$, P. phenazinium $994.34{\mu}g\;mL^{-1}$의 순으로 나타나 두 종의 인산가용화 박테리아간의 동시 접종에 의한 시너지효과가 관찰되었다. 배지 내 pH 와 잔류 glucose 함량 변화도 모든 접종구에서 배양 1일 후 대부분의 변화가 이루어져 pH의 경우 초기 pH 7.0에서 pH 4.0 수준으로 감소하였으며, 잔류 glucose 함량은 초기 $10mg\;mL^{-1}$에서 $4.8mg\;mL^{-1}$ 수준으로 검출되어 인산가용화능과 거의 유사한 경향을 보였다. 배양여액의 유기산 분석결과 gluconic acid 약 $11mg\;mL^{-1}$와 malic acid 약 $4mg\;mL^{-1}$ 수준으로 가장 높게 검출되었으며, Park et al(2016)에 의해 보고된 oxalic acid는 검출되지 않았다. 대부분의 유기산이 배양 1일과 3일 후 생성되는 결과를 보임으로서 유기산이 배지의 pH를 감소시키고 인산 가용화를 유도하는 주요 원인임을 확인할 수 있었다. 접종 4주 후 상추 생육차이를 조사한 결과, TCP를 첨가하지 않은 P. phenazinium 접종구, P. metrosideri 접종구 와 동시 접종구에 비해 TCA 첨가한 모든 처리구에서 10 -20% 수준의 생육증진효과가 확인되었으나 단일접종과 동시접종구간에 유의수준의 차이를 확인할 수 없었다. 결과적으로 인(P) 결핍 토양 및 염류토양에 인산가용화균을 사용하면 식물과 작물 수확량에 의한 P 섭취가 동시에 증가하고 생육이 촉진된다는 다른 연구결과(Rodriguez et al, 1999; Suh et al, 2008; Walpola and Yoon. 2013)와 유사한 경향을 보였다.

BSHGBD_2019_v17n2_64_f0001.png 이미지

Fig. 1. Phylogenetic tree based on 16S rDNA gene sequences, showing the position of isolate phosphate solubilizing bacterial strains YH3 (PSB-C) with respect to related species. The scale bar dindicates 0.005 substitutions per nucleotide position and accession numbers are given in parenthesis.

BSHGBD_2019_v17n2_64_f0002.png 이미지

Fig. 2. Phylogenetic tree based on 16S rDNA gene sequences, showing the position of isolate phosphate solubilizing bacterial strains YH4 (PSB-D) with respect to related species. The scale bar dindicates 0.005 substitutions per nucleotide position and accession numbers are given in parenthesis.

BSHGBD_2019_v17n2_64_f0003.png 이미지

Fig. 3. Comparison of phosphate solubilization between single and co-inoculation of P. phenazinium and P. metrosideri. Values given here are the means of three replicates (n=3).

BSHGBD_2019_v17n2_64_f0004.png 이미지

Fig. 4. Comparison of pH change during single and coinoculation of P. phenazinium and P. metrosideri. Values given here are the means of three replicates (n=3).

BSHGBD_2019_v17n2_64_f0005.png 이미지

Fig. 5. Comparison of residual glucose in the medium between single and co-inoculation of P. phenazinium and P. metrosideri. Values given here are the means of three replicates (n=3)

Table 1. HPLC analysis of organic acid by single and co-inoculation of (Paraburkholderia phenazinium and Paraburkholderia metrosideri) Values given here are the means of three replicates (n=3). ND = Not dectected.

BSHGBD_2019_v17n2_64_t0001.png 이미지

Table 2. Comparison on growth of romaine lettuce after 4 weeks inoculation with Paraburkholderia phenazinium and Paraburkholderia metrosideri. Values given here are the means of three replicates (n=3).

BSHGBD_2019_v17n2_64_t0002.png 이미지

참고문헌

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