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Physiological activities of extracts of wild mushrooms collected in Korea

국내 야생수집 버섯류 추출물의 생리활성 분석

  • An, Gi-Hong (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Jae-Han (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Kang-Hyo (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Han, Jae-Gu (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 안기홍 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 조재한 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이강효 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 한재구 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과)
  • Received : 2019.05.13
  • Accepted : 2019.06.20
  • Published : 2019.06.30

Abstract

This study was carried out to analyze the physiological activities of wild mushroom extracts collected from the Gangwon-do, Gyeonggi-do, and Chungcheongbuk-do provinces in Korea. Among the wild mushroom extracts, those of Clitocybe robusta and Leucopaxillus giganteus (OK829) showed the highest DPPH radical scavenging activities. Nitrite scavenging activity of the L. giganteus extract (OK811) was determined to be 64.1%, which is considerably higher than those of the other mushroom extracts analyzed in this study. The total polyphenol levels in Suillus granulatus, L. giganteus (OK829), and Amanita manginiana extracts were found to be 19.7 mg GAE/g, 20.2 mg GAE/g, and 22.3 mg GAE/g, respectively. To determine their anti-inflammatory effects, nitric oxide production, and cell viability, NO measurement and MTT assays were performed using lipopolysaccharide (LPS)-treated RAW 264.7 cells. The levels of nitric oxide (NO) produced by the C. robusta and Hypholoma fasciculare extracts were remarkably lower than those produced by the others. In our MTT assay, the extracts of S. granulatus, L. giganteus (OK811), and Lactarius chrysorrheus showed high cell viabilities of 40.3%, 48.3%, and 43.2%, respectively. These results can provide the fundamental data for extracting useful compounds from wild mushrooms.

Keywords

DPPH scavenging activity;MTT assay;Nitrite scavenging activity;NO assay;Total pholyphenol content;Wild mushroom

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Fig. 1. Fruiting bodies of the wild mushrooms in this study (A, OK795; B, OK804; C, OK811; D, OK825; E, OK826; F, OK829; G, OK832; H, OK904; I, OK943; J, OK944).

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Fig. 2. DPPH radical scavenging activities of wild mushroom extracts (1mg/ml concentrations). White bar indicates a positive control. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

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Fig. 3. Nitrite scavenging activities of wild mushroom extracts (1mg/ml concentrations). White bar indicates a positive control. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

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Fig. 4. Total polyphenol contents of wild mushroom extracts (1mg/ml concentrations). White bar indicates a negative control. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

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Fig. 5. The suppression rates of nitric oxide (NO) by the treatment of wild mushrooms extracts were determined by using NO assay. RAW 264.7 cells were incubated under 1 μg/ml concentration of lipopolysaccharide (LPS) and wild mushrooms extracts. White bars indicate positive and negative controls. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

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Fig. 6. The rates of cell viability after treating wild mushrooms extracts. Viability of RAW 264.7 cells harvest at 24 hr after 1 μg/ml concentration of LPS addition was determined using MTT assay. White bars indicate positive and negative controls. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

Table 1. List of the strains/specimens used in this study.

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Acknowledgement

Supported by : 국립원예특작과학원

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