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A Study on the Expression of Thymosin-β4 and c-Myc mRNA in the Model of liver cirrhosis with fibrosis

섬유화 진행 간경변 조직 모델에서 Thymosin β4와 C-myc mRNA 융합 발현 연구

  • Kim, Jean-Soo (Department of Clinical Laboratory Science, Daejeon Health Institute of Technology) ;
  • Park, Un-Kyu (Department of Clinical Laboratory Science, Daejeon university)
  • 김진수 (대전보건대학교 임상병리과) ;
  • 박운규 (대전대학교 임상병리과)
  • Received : 2019.03.28
  • Accepted : 2019.06.20
  • Published : 2019.06.28

Abstract

The propose of this study has been conducted to examine expression of c-Myc and Thymosin-${\beta}4$ in liver cirrhosis model from liver fibrosis and For the method of study, the experiment was conducted in 2 groups; liver cirrhosis model experiment group due to liver fibrosis and control group with distilled water. This study outcome showed that liver cirrhosis model experiment group had significantly higher expression of c-Myc and Thymosin-${\beta}4$. with changes to hepatic tissue of special staining and electron microscopy. In conclusion, in clinical tests regarding liver function, molecular evaluation of c-Myc and Thymosin-${\beta}4$ and their expression along with serological change and histological assessment can be utilized as a reference for diagnosing liver disease for prevention and diagnosis of the disease, Based on this research in the future, we will carry out an in-depth study by adding the types of experimental groups and related genes.

Keywords

Hematoxylin;Eosin;Masson's Trichrome;Reticulum;c-Myc;Thymosin-${\beta}4$;Convergence

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Fig. 1. Shapes of liver tissue in the TransmissionElectron Microscope.

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Fig. 2. H & E stain of liver tissue.

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Fig .3. Masson's Trichrom stain of liver tissue.

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Fig. 4. Reticular fiber stain of liver tissue.

Table 1. Method of Hematoxylin & Eosin stain

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Table 2. Transmission Electron Microscope : Method of TEM stain

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Table 3. Method of Masson's Trichrom stain

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Table 4. Method of Gomori Reticulum stain

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Table 5. Primers used for the detection of gene expression

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Table 6. Relative expression of β-actin, c-Myc and Thymosin-β4 gene expression

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Table 7. Cq values of gene expression in groups administered with CCl4

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Acknowledgement

Supported by : Daejeon Health Institute of Technology

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