Isolation and Characterization of Novel Chlorella Species with Cold Resistance and High Lipid Accumulation for Biodiesel Production

  • Koh, Hyun Gi (Advanced Biomass R&D Center (ABC), KAIST) ;
  • Kang, Nam Kyu (Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign) ;
  • Kim, Eun Kyung (Advanced Biomass R&D Center (ABC), KAIST) ;
  • Suh, William I. (Advanced Biomass R&D Center (ABC), KAIST) ;
  • Park, Won-Kun (Department of Chemistry and Energy Engineering, Sangmyung University) ;
  • Lee, Bongsoo (Department of Microbial and Nano Materials, College of Science and Technology, Mokwon University) ;
  • Chang, Yong Keun (Advanced Biomass R&D Center (ABC), KAIST)
  • Received : 2019.04.04
  • Accepted : 2019.05.17
  • Published : 2019.06.28


Chlorella spp. are green algae that are found across wide-ranging habitats from deserts to arctic regions, with various strains having adapted to survive under diverse environmental conditions. In this study, two novel Chlorella strains (ABC-002, ABC-008) were isolated from a freshwater lake in South Korea during the winter season and examined for possible use in the biofuel production process. The comparison of ABC-002 and ABC-008 strains with Chlorella vulgaris UTEX265 under two different temperatures ($10^{\circ}C$, $25^{\circ}C$) revealed their cold-tolerant phenotypes as well as high biomass yields. The maximum quantum yields of UTEX25, ABC-002, and ABC-008 at $10^{\circ}C$ were 0.5594, 0.6747, and 0.7150, respectively, providing evidence of the relatively higher cold-resistance capabilities of these two strains. Furthermore, both the biomass yields and lipid content of the two novel strains were found to be higher than those of UTEX265; the overall lipid productivities of ABC-002 and ABC-008 were 1.7 ~ 2.8 fold and 1.6 ~ 4.2 fold higher compared to that of UTEX265, respectively. Thus, the high biomass and lipid productivity over a wide range of temperatures indicate that C. vulgaris ABC-002 and ABC-008 are promising candidates for applications in biofuel productions via outdoor biomass cultivation.


Supported by : Advanced Biomass R&D Center (ABC)


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