Fig. 1. Preparation of crude extracts and their solvent-patitioned fractions from A. gmelinii
Fig. 2. Effect of A. gmelinii crude extracts (A) and their solvent fractions (B) on cell viability observed by MTT assay.
Fig. 3. Inhibitory effects of A. gmelinii crude extracts and their solvent-partitioned fractions on secretion levels of MMP-2 (A and C) and MMP-9 (B and D) were observed by ELISA kit.
Fig. 4. Inhibitory effects of the combined crude extracts (C.E) of A. gmelinii on enzymatic activity (A), mRNA expression (B), and protein (C) expression of MMP-2 and MMP-9 were observed by gelatin zymography, RTPCR, and Western blot assay, respectively.
Fig. 5. Inhibitory effects of solvent-partitioned fractions from A. gmelinii crude extracts were observed in enzymatic activity (A), mRNA expression (B), and protein expression (C) of MMP-2 and MMP-9 by gelatin zymography, RT-PCR and Western blot assay, respectively, and in HT-1080 cell migration (D)
Table 1. Sequences of primers used for RT-PCR