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The effects of Socheongryong-Tang on LPS-induced lung inflammation rats model

소청룡탕이 LPS로 유도된 폐손상 동물모델에 미치는 영향

  • Jin, Bo-Ram (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Choi, In Young (Korean Medicinal Herbs Standardization Team, National Institute for Korean Medicine Development) ;
  • Hwang, Do Young (Korean Medicinal Herbs Standardization Team, National Institute for Korean Medicine Development) ;
  • Ham, Seong-Ho (Korean Medicinal Herbs Standardization Team, National Institute for Korean Medicine Development) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
  • 진보람 (상지대학교 한의과대학 약리학교실) ;
  • 최인영 (한국한의약진흥원 한약재표준화팀) ;
  • 황도영 (한국한의약진흥원 한약재표준화팀) ;
  • 함성호 (한국한의약진흥원 한약재표준화팀) ;
  • 안효진 (상지대학교 한의과대학 약리학교실)
  • Received : 2019.08.22
  • Accepted : 2019.09.25
  • Published : 2019.09.30

Abstract

Objectives : In present study, we investigated a therapeutic effect and optimum dose of Socheongryong-Tang (SCT) on LPS-induced lung inflammation rats model. Methods : Male Sprague-Dawley rats ($260{\pm}10g$) were divided into 12 groups : Group 1 included the normal rats, and Group 2-12 were administrated LPS by intranasal injection to induce experimental lung inflammation. After 1 day of LPS administration, Group 3-9 were treated with SCT ${\times}1/4$, ${\times}1/2$, ${\times}1$, ${\times}3$, ${\times}6$, ${\times}12$ or ${\times}18$, respectively. Group 10-12 (positive control) were treated with dexamethasone 1 mg/kg or acetylcystein 1.5 mg/kg or diclofenac sodium 0.4 mg/kg, respectively. After sacrifice, bronchoalveolar lavage fluid (BALF) was isolated. The levels of IL-$1{\beta}$, TNF-${\alpha}$, mucin glycoprotein 5AC (MUG5AC) were measured in BALF using enzyme-linked immunosorbent assay (ELISA). Results : LPS injected rats exhibited outstanding lung inflammation manifestations, including increased amount of total cells and neutrophil, and upregulated inflammatory cytokines level in BALF. However, the administration of SCT ${\times}1/4$, ${\times}1/2$ and ${\times}1$ decreased total cells and neutrophil, and suppressed the production of inflammatory cytokines, including $IL-1{\beta}$ and TNF-${\alpha}$, and MUG5AC in BALF. Notably, inhibitory effect of SCT ${\times}1/2$ and ${\times}1$ on the level of TNF-${\alpha}$ was markedly better than that of positive controls, dexamethasone and acetylcystein. Conclusions : Taken together, these results suggest that SCT ${\times}1/2$ and ${\times}1$ has therapeutic effects on LPS-induced lung inflammation rats model.

Keywords

Socheongryong-Tang;LPS-induced lung inflammation rats model;SD rats

Acknowledgement

Supported by : Ministry of Health and welfare (MOHW)

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