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The Suppressive Effects of Daebangfung-tang against Disuse Muscle Atrophy in Gastrocnemius of Rats

대방풍탕의 흰 쥐 비복근 불용성 근위축에 대한 억제 효과

  • Kim, Jae Hoon (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jung Hee (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Cho In (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Bong Hyo (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Hyun-Jong (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daegu Haany University)
  • 김재훈 (대구한의대학교 부속 한방병원 침구의학과) ;
  • 이정희 (대구한의대학교 부속 한방병원 침구의학과) ;
  • 이초인 (대구한의대학교 부속 한방병원 침구의학과) ;
  • 이봉효 (대구한의대학교 부속 한방병원 침구의학과) ;
  • 이윤규 (대구한의대학교 부속 한방병원 침구의학과) ;
  • 이현종 (대구한의대학교 부속 한방병원 침구의학과) ;
  • 김재수 (대구한의대학교 부속 한방병원 침구의학과)
  • Received : 2020.11.13
  • Accepted : 2020.12.11
  • Published : 2020.12.27

Abstract

Objectives : The aim of this study is to evaluate the effect of Daebangfung-tang (DBFT) on suppressing muscle atrophy followed by immobilization. Methods : Twenty young male Sprague-Dawley rats were randomized into two groups. The rats in experimental group (DBFT group) were orally administrated DBFT once a day for 2 weeks, and the rats in control group were given normal saline in the same manner. The immobilization on left leg was carried out using casting tape. The morphologies of gastrocnemius in both groups were assessed. The cross sectional area of them was measured by image analyzer. Immunohistochemical staining was implemented and the numbers of immunopositive cells implying the expression of TNF-α were measured and quantified. Results : DBFT represented the significant suppressive effects against the weight loss of the left gastrocnemius muscle and the reduction in cross sectional area of the left gastrocnemius muscle compared with normal saline. Also the treatment with DBFT significantly reduced the expression of TNF-α in gastrocnemius compared with normal saline. Conclusions : DBFT is thought to have the significant suppressive effect against muscle atrophy followed by immobilization.

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