DOI QR코드

DOI QR Code

Pathological, immunohistochemical, and bacteriological findings in dogs infected with Brucella canis

  • Jung, Ji-Youl (Animal Disease Diagnostic Research Division, Animal and Plant Quarantine Agency) ;
  • Yoon, Soon-Seek (Animal Disease Diagnostic Research Division, Animal and Plant Quarantine Agency) ;
  • Lee, Seunghee (Animal Disease Diagnostic Research Division, Animal and Plant Quarantine Agency) ;
  • Park, Jung-Won (Animal Disease Diagnostic Research Division, Animal and Plant Quarantine Agency) ;
  • Lee, JinJu (Bacterial Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Her, Moon (Research Planning & Management Division, Animal and Plant Quarantine Agency) ;
  • So, ByungJae (Animal Disease Diagnostic Research Division, Animal and Plant Quarantine Agency) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Received : 2019.09.16
  • Accepted : 2019.12.02
  • Published : 2020.03.31

Abstract

This study describes pathological, immunohistochemical, and bacteriological findings in adult dogs and fetuses naturally infected with Brucella (B.) canis. A total of 42 dogs including 40 dogs and 2 aborted fetuses were examined. The most common gross lesions in infected dogs were swelling of lymph nodes and spleen. The testes showed marked swelling with multifocal to diffuse reddish discoloration. The most significant histopathological lesions were observed in the placenta. Placental trophoblasts were markedly hypertrophied due to the accumulation of intra-cellular gram-negative bacteria. Lymphocytic inflammation of varying severity was observed in the reproductive organs such as male testis, epididymis, and prostate gland and female uterus. Strong immunolabelling was observed in the cytoplasm of most trophoblasts in the placental tissues using immunohistochemistry. However, immunohistochemical staining did not demonstrate any organisms in other organs of dogs and fetuses. B. canis isolates were most frequently obtained from the whole blood (82.5%) and superficial inguinal lymph node (77.5%) in both sexes. In addition, the isolation rate was higher in male genital organs than in those of females. Hence, management of male dogs is most important because infected dogs can play a role as carriers.

Acknowledgement

Supported by : Animal and Plant Quarantine Agency

References

  1. Carmichael LE. Brucella canis. In: Nielsen KH, Duncan JR (eds.). Animal Brucellosis. pp. 336-350, CRC Press, Boca Raton, 1990.
  2. Gyuranecz M, Szeredi L, Ronai Z, Denes B, Dencso L, Dan , Palmai N, Hauser Z, Lami E, Makrai L, Erdelyi K, Janosi S. Detection of Brucella canis-induced reproductive diseases in a kennel. J Vet Diagn Invest 2011;23:143-147. https://doi.org/10.1177/104063871102300127
  3. Wanke MM. Canine brucellosis. Anim Reprod Sci 2004;82-83:195-207. https://doi.org/10.1016/j.anireprosci.2004.05.005
  4. Rodriguez Ferri EF, Suarez Fernandez G, Dominguez Rodriguez L, Pozo Vegas M. Estudio serologico de perros procedentes de areas urbanas y rurales en relacion con la brucelosis. An Inst Nac Investig Agrar Ser Ganad 1982;17:123-139.
  5. Carmichael LE, Kenney RM. Canine abortion caused by Brucella canis. J Am Vet Med Assoc 1968;152:605-616.
  6. Kim SG, Seo HJ, Kim ST, Jang YS, Jo MH. Serological and bacteriological study on canine brucellosis in the large kennel farms in Gyeongbuk province. Korean J Vet Serv 2010;33:129-134.
  7. Kang SI, Her M, Kim JW, Kim JY, Ko KY, Ha YM, Jung SC. Advanced multiplex PCR assay for differentiation of Brucella species. Appl Environ Microbiol 2011;77:6726-6728. https://doi.org/10.1128/AEM.00581-11
  8. Xavier MN, Paixao TA, Poester FP, Lage AP, Santos RL. Pathological, immunohistochemical and bacteriological study of tissues and milk of cows and fetuses experimentally infected with Brucella abortus. J Comp Pathol 2009;140:149-157. https://doi.org/10.1016/j.jcpa.2008.10.004
  9. Anderson JD, Smith H. The metabolism of erythritol by Brucella abortus. J Gen Microbiol 1965;38:109-124. https://doi.org/10.1099/00221287-38-1-109
  10. Hollett RB. Canine brucellosis: outbreaks and compliance. Theriogenology 2006;66:575-587. https://doi.org/10.1016/j.theriogenology.2006.04.011
  11. Greene CE, Carmichael LE. Canine brucellosis. In: Greene CE (ed.). Infectious Diseases of the Dog and Cat. 4th ed. pp. 398-411, WB Saunders, Philadelphia, 2011.
  12. Hofer E, Bag ZN, Revilla-Fern Ndez S, Melzer F, Tomaso H, L Pez-Go I I, Fasching G, Schmoll F. First detection of Brucella canis infections in a breeding kennel in Austria. New Microbiol 2012;35:507-510.
  13. Keid LB, Soares RM, Vieira NR, Megid J, Salgado VR, Vasconcellos SA, da Costa M, Gregori F, Richtzenhain LJ. Diagnosis of canine brucellosis: comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspacer. Vet Res Commun 2007;31:951-965. https://doi.org/10.1007/s11259-006-0109-6