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Cytotoxicity of natural killer cells on canine mammary carcinoma cells

개 유선종양세포에 대한 자연살해세포 독성

  • Jeong, Da-Un (Viral Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Byeon, Jeong Su (Viral Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Gu, Na-Yeon (Viral Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Jung, Moonhee (Department of Forensic Medicine, Chonnam National University Medical School) ;
  • Kim, Eun Hee (Department of Forensic Medicine, Chonnam National University Medical School) ;
  • Kim, Hyung-Seok (Department of Forensic Medicine, Chonnam National University Medical School) ;
  • Cho, In-Soo (Viral Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Song, Jae-Young (Viral Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Hyun, Bang-Hun (Viral Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Lee, Jienny (Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • 정다운 (농림축산검역본부 바이러스질병과) ;
  • 변정수 (농림축산검역본부 바이러스질병과) ;
  • 구나연 (농림축산검역본부 바이러스질병과) ;
  • 정문희 (전남대학교 의과대학 법의학교실) ;
  • 김은희 (전남대학교 의과대학 법의학교실) ;
  • 김형석 (전남대학교 의과대학 법의학교실) ;
  • 조인수 (농림축산검역본부 바이러스질병과) ;
  • 송재영 (농림축산검역본부 바이러스질병과) ;
  • 현방훈 (농림축산검역본부 바이러스질병과) ;
  • 이지현 (농림축산검역본부 바이러스질병과)
  • Received : 2019.06.28
  • Accepted : 2019.12.02
  • Published : 2020.03.31

Abstract

Natural killer (NK) cells play have a crucial role in the early phase of immune responses against various pathogens. We compared characteristics of canine NK cells against two canine mammary carcinoma cell lines, REM134 and CF41.Mg. REM134 showed higher expression of progesterone receptor, proliferative cell nuclear antigen, Ki67, multiple drug resistance, Bmi-1, c-myc, E-cadherin, and human epidermal growth factor receptor type-2 than that of CF41.Mg. For specific expansion and activation of NK cells, we isolated CD5 negative cells from canine peripheral blood mononuclear cells and co-cultured K562 cells in the presence of interleukin (IL)-2, IL-15, and IL-21 for 21 days. As a result, we found that expression markers of activated NK cells such as NKp30, NKp44, NKp46, NKG2D, CD244, perforin, granzyme B, and tumor necrosis factor alpha were highly upregulated. In addition, we found there was upregulated production of interferon gamma of activated NK cells against target cells such as REM134 and CF41.Mg. Specifically, we observed that cytotoxicity of NK cells against target cells was more sensitively reacted to CF41.Mg than REM134. Based on the results of this study, we recommend the development of an experimental application of CF41Mg, which has not been reported in canine mammary carcinoma research.

Acknowledgement

Supported by : 농림축산검역본부

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