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The Skin Whitening Effect of Gypsum Extract using B16F10 cell lines

석고 추출물의 미백 효과

  • Kam, Eun-Young (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kang, Eun-Jeong (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Ryu, Ji-Yeon (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Park, Soo-Yeon (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Jung, Min-Yeong (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kim, Jong-Han (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Choi, Jung-Hwa (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
  • 감은영 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 강은정 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 류지연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 김종한 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2020.04.02
  • Accepted : 2020.05.05
  • Published : 2020.05.25

Abstract

Objectives : Recently the aesthetic, especially skin whitening, is focused on. This study was designed to investigate the effects of Gypsum Extract(GE) on skin whitening using B16F10 cell lines. Methods : In this study, we investigated effect of GE on cell viability, melanin synthesis, Superoxide dismutase(SOD)-like activity and tyrosinase activity in vitro and in vivo. Results : In results, The proliferation of B16F10 cell lines was increased by GE concentration. But there was no statistical signification. Melanin synthesis was decreased except GE 125㎍/㎖ of treated group. And the melanin synthesis ratio was increased in group being treated α-MSH. But After treating GE, it was decreased compared to Non-GE treated group. In addition, the SOD-like activity was enhanced in more than GE 125㎍/㎖ of treated group. And the activity of tyrosinase in vitro was inhibited in GE 500㎍/㎖ and 1,000㎍/㎖ of treated groups. The activity of tyrosinase in vivo was reduced in both Arbutin 500㎍/㎖ of treated group and Gypsum 500㎍/㎖ of treated group compared to controled group. After being treated α-MSH, the activity of tyrosinase was enhanced. But after treating Arbutin 500㎍/㎖ or Gypsum 500㎍/㎖, it was inhibited being compared to the group of Non-medicament treated. Conclusion : The results of this study showed the effects of GE that can inhibit melanin synthesis and the activity of tyrosinase and enhance the SOD-like activity. It means that gypsum has the skin whitening effect.

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