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The effects of Lactobacillus reuteri-containing probiotics on the viability and biofilm formation of oral microorganisms

Lactobacillus reuteri 함유 Probiotics가 구강미생물의 생존 및 biofilm 형성에 미치는 영향

  • Lee, Su-Bin (Department of Health Science, Dongseo University) ;
  • Lee, Kyung-Hee (Department of Dental Hygiene, Dongseo University)
  • 이수빈 (동서대학교 일반대학원 보건과학과) ;
  • 이경희 (동서대학교 치위생학과)
  • Received : 2020.04.23
  • Accepted : 2020.06.02
  • Published : 2020.06.30

Abstract

Objectives: This study aimed to evaluate the inhibitory effects of probiotics containing Lactobacillus reuteri on Streptococcus mutans and Aggregatibacter actinomycetemcomitans. In addition, the degree of biofilm formation, initial acidity, buffering ability, and acid production performance were measured to confirm the dental caries-inducing ability. Methods: S. mutans (KCTC3065) and A. actinomycetemcomitans (KCTC2581) were used as experimental strains. The number of viable cells, degree of biofilm formation, initial pH, buffering capacity, and production performance were measured for comparing L. reuteri-containing probiotics and Bulgaris. Results: The viability of S. mutans in the groups was reduced in the following order: Bulgaris, probiotics, control. The degree of biofilm formation was significantly higher at 0% and gradually reduced at different concentrations (p<0.01). At 2.5%, the absorbance of the probiotics and Bulgaris groups differed significantly (p<0.01). The acid formation ability differed significantly based on the performance of S. mutans in each product (p<0.05). The absorbance of the probiotics group was significantly lower than that of the Bulgaris group (p<0.01). Conclusions: This study suggests that the use of L. reuteri-containing probiotics as an adjuvant for the prevention and decreasing of oral diseases may reduce their incidence, which can be considered one of the benefits of using probiotics.

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