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Development of Multi-residue Analytical Method for 261 Pesticides in Herbal Medicines using GC-MS/MS and LC-MS/MS

GC-MS/MS와 LC-MS/MS를 이용한 생약재 중 261종 농약의 동시분석

  • Na, Eun Shik (R&D Headquarters, Korea Ginseng Corporation) ;
  • Kim, Seong Soo (R&D Headquarters, Korea Ginseng Corporation) ;
  • Hong, Sung Soo (R&D Headquarters, Korea Ginseng Corporation) ;
  • Kim, Kyoung Ju (R&D Headquarters, Korea Ginseng Corporation) ;
  • Lee, Yong Jae (R&D Headquarters, Korea Ginseng Corporation) ;
  • Lee, Byung Chul (R&D Headquarters, Korea Ginseng Corporation) ;
  • Lee, Kyu Seung (Department of Biological Environment Chemistry, College of Agriculture, Life and Science, Chungnam National University)
  • 나은식 ((주)한국인삼공사 R&D본부) ;
  • 김성수 ((주)한국인삼공사 R&D본부) ;
  • 홍성수 ((주)한국인삼공사 R&D본부) ;
  • 김경주 ((주)한국인삼공사 R&D본부) ;
  • 이용재 ((주)한국인삼공사 R&D본부) ;
  • 이병철 ((주)한국인삼공사 R&D본부) ;
  • 이규승 (충남대학교 농업생명과학대학 생물환경화학과)
  • Received : 2020.04.21
  • Accepted : 2020.06.24
  • Published : 2020.06.30

Abstract

BACKGROUND: A new analytical method has been developed to determine 261 pesticide residues in herbal medicines. METHODS AND RESULTS: The extraction of pesticides was carried out by modified method of the Korea Food Standards Codex sample extraction and determination was performed using GC-MS/MS and LC-MS/MS. During the pre-treatment process of the test method, Solid-liquid separation was changed to centrifugation. The method was validated by the precision and accuracy results. 261 pesticides spiked at three level 20, 50, 100 ug/kg in herbal medicines. The limit of quantification of method were 4-40 ug/kg for GC-MS/MS and 2-45 ug/kg for LC-MS/MS, respectively. Among the pesticides analysed by GC-MS/MS and LC-MS/MS, 244 pesticides (94% of total number) in chinese matrimony vine and 224 pesticides (86% of total number) in korean angelica root and 231 pesticides (89% of total number) in jujube and 214 (82% of total number) in cnidium showed recoveries in the range of 70-120% with RSD⪯20%. CONCLUSION: These results indicated that GC-MS/MS and LC-MS/MS analysis with the sample extraction in this study can be applied to multi-residue analysis of pesticides in herbal medicines.

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