- Volume 39 Issue 2
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Development of Multi-residue Analytical Method for 261 Pesticides in Herbal Medicines using GC-MS/MS and LC-MS/MS
GC-MS/MS와 LC-MS/MS를 이용한 생약재 중 261종 농약의 동시분석
- Na, Eun Shik (R&D Headquarters, Korea Ginseng Corporation) ;
- Kim, Seong Soo (R&D Headquarters, Korea Ginseng Corporation) ;
- Hong, Sung Soo (R&D Headquarters, Korea Ginseng Corporation) ;
- Kim, Kyoung Ju (R&D Headquarters, Korea Ginseng Corporation) ;
- Lee, Yong Jae (R&D Headquarters, Korea Ginseng Corporation) ;
- Lee, Byung Chul (R&D Headquarters, Korea Ginseng Corporation) ;
- Lee, Kyu Seung (Department of Biological Environment Chemistry, College of Agriculture, Life and Science, Chungnam National University)
- 나은식 ((주)한국인삼공사 R&D본부) ;
- 김성수 ((주)한국인삼공사 R&D본부) ;
- 홍성수 ((주)한국인삼공사 R&D본부) ;
- 김경주 ((주)한국인삼공사 R&D본부) ;
- 이용재 ((주)한국인삼공사 R&D본부) ;
- 이병철 ((주)한국인삼공사 R&D본부) ;
- 이규승 (충남대학교 농업생명과학대학 생물환경화학과)
- Received : 2020.04.21
- Accepted : 2020.06.24
- Published : 2020.06.30
BACKGROUND: A new analytical method has been developed to determine 261 pesticide residues in herbal medicines. METHODS AND RESULTS: The extraction of pesticides was carried out by modified method of the Korea Food Standards Codex sample extraction and determination was performed using GC-MS/MS and LC-MS/MS. During the pre-treatment process of the test method, Solid-liquid separation was changed to centrifugation. The method was validated by the precision and accuracy results. 261 pesticides spiked at three level 20, 50, 100 ug/kg in herbal medicines. The limit of quantification of method were 4-40 ug/kg for GC-MS/MS and 2-45 ug/kg for LC-MS/MS, respectively. Among the pesticides analysed by GC-MS/MS and LC-MS/MS, 244 pesticides (94% of total number) in chinese matrimony vine and 224 pesticides (86% of total number) in korean angelica root and 231 pesticides (89% of total number) in jujube and 214 (82% of total number) in cnidium showed recoveries in the range of 70-120% with RSD⪯20%. CONCLUSION: These results indicated that GC-MS/MS and LC-MS/MS analysis with the sample extraction in this study can be applied to multi-residue analysis of pesticides in herbal medicines.
- Choi YC, Park SK, Kim OH, Seoung HJ, Han SH, Lee YJ, Jeong HJ, Kim YH, JO HB et al. (2011) Pesticides residue monitoring of medicinal herbs in Seoul. The Korean Jounral of Pesticide Science, 15(4), 335-349.
- Cho JH, KIM DH, Kim HS, Oh MH, Kang IH, Shim YH, Hwang WK, Myung SW, Chio BK (2000) Monitoring research for residual pesticides as endocrine disreptors in natural medicines(I). Korean Journal of Pharmacognosy, 31(4), 455-458.
- Hwang BH, Lee MR (2000) Solid-phase microextraction for organochlorine pesticide residues analysis in Chinese herbal formulations. Journal of Chromatography A, 898(2), 245-256.
- Lee SH, Kim HS, Kim YM, KIM WS, Won YJ, Chae GY, Kim OH, Park HJ, Jeong SW (2006) Monitoring of pesticide residues in herbal medicines. Journal of the Environmental Sciences, 15(8), 811-817.
- KIM BS, Park SG, Kim MS, Cho TH, Han CH, Jo HB, Chio BH, Kim SD (2007) A study of current status on pesticide residues in commercial dried agricultural products. Korean Jounral Food Science and Technology, 39(2), 114-121.
- Ock HS (2009) Developmental trend of analytical methods for pesticide residues. The Korean Jounral of Pesticide Science, 13(4), 336-348.
- Park JW, Kim AK, Kim JP, Lee HH, Park DW, Moon SJ, Ha DY, Seo KW (2014) Multi-residue analysis of pesticides using GC-TOF/MS, ECD, NPD with QuEChERS sample preparation. The Korean Jounral of Pesticide Science, 18(4), 278-295.
- Wilkowska A, Biziuk M (2011) Determination of pesticide residues in food matrices using QuEChERS methodology. Food Chemistry, 125(3), 803-812. https://doi.org/10.1016/j.foodchem.2010.09.094.
- de Sousa Freitas S, Lancas FM (2009) Matrix effects observed during pesticides residue analysis in fruits by GC. Journal of Seperation Science, 32(21), 3698-3705. https://doi.org/10.1002/jssc.200900358.
- Hajslova, J., Holadova, K., Kocourek, V., Poustka, J., Godula, M., Cuhra, P., & Kempny, M. (1998). Matrix-induced effects: a critical point in the gas chromatographic analysis of pesticide residues. Journal of Chromatography A, 800(2), 283-295.
- Kwon HY, Kim CS, Park BJ, Jin YD, Son KA (2011) Multiresidue analysis of 240 pesticides in apple and lettuce by QuEChERS sample preparation and HPLC-MS/MS analysis. The Korean Jounral of Pesticide Science, 15(4), 417-433.
- Hernando MD, Suarez-Barcena JM, Bueno MJM, Garcia-Reyes JF, Fenandez-Alba AR (2007) Fast separation liquid chromatography tandem mass spectrometry for the confirmation and quantitative analysis of avermectin residues in food. Journal of chromatograpy A, 1155(1), 62-73. https://doi.org/10.1016/j.chroma.2007.02.120.
- Ferrer C, Lozano A, Aguera A, Giron AJ, Fernandez-Alba AR (2011) Overcoming matrix effects using the dilution approach in multiresidue methods for fruits and vegetables. Journal of Chromatograpy A, 1218(42), 7634-7639. https://doi.org/10.1016/j.chroma.2011.07.033.
- Moreno-Gonzalez D, Huertas-Perez JF, Garcia-Campana AM, Gamiz-Gracia L (2014) Determination of carbamates in edible vegetable oils by ultra-high performance liquid chromatography-tandem mass spectrometry using a new clean-up based on zirconia for QuEChERS methodology. Talanta, 128, 299-304. https://doi.org/10.1016/j.talanta.2014.04.045.
- Gwon JH, Kim TK, Seo EK, Hong SM, Kwon KS, Kyung HY, Kyung KS, Kim JE, Cho NJ (2014) Multiresidue analysis of 124 pesticides in soil with QuEChERS extraction and LC-MS/MS. The Korean Jounral of Pesticide Science, 18(4), 296-313.
- Stahnke H, Reemtsma T, Alder L (2009) Compensation of matrix effects by postcolumn infusion of a monitor substance in multiresidue analysis with LC-MS/MS. Analytical Chemistry, 81(6), 2185-2192. https://doi.org/10.1021/ac802362s.