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Molecular & cellular toxicology
Volume 4, Issue 3, September 2008, pp.199-204
The Korean Society of Toxicogenomics and Toxicoproteomics
Subject Category : Technology (Applied sciences) / Medical sciences Medicine
ISSN : 1738-642x

Differential Gene Expression in a Red Alga Gracilaria textorii(Suringar) Hariot (Gracilariales, Florideophyceae) between Natural Populations Woo, Seon-Ock ; Ko, Young-Wook ; Oh, Yoon-Sik ; Kim, Jeong -Ha ; Lee, Taek-Kyun ; Yum, Seung-Shic

Abstract	The bio-molecules involved in defense mechanisms can be used as efficient biomarkers for physiological changes in organisms caused by both of internal and external stress. Thus, the expression level of genes which encoding such molecules serve as critical 'early warning system' for environmental assessment as well as health diagnosis of biological organisms. In this study, Cytochrome P450, Heat shock protein 90, Ubiquitin and ${${\beta}$}$ -actin gene were isolated for the first time from a red alga Gracilaria textorii. The quantitative differential gene expression analyses of three genes, GteCYP1A, GteHsp90 and Gte-UB, were carried out in G. textorii sporophytes collected from two different localities, polluted Sujeong (Masan, Korea) and potentially unpolluted Danggeum (Daemaemuldo Is., Korea). The transcripts of all three tested genes were highly expressed in the Sujeong population. The results suggest: 1) the Sujeong site was more polluted than the Danggeum site; 2) G. textorii could be applicable to marine environment monitoring in coastal regions.

Keywords	Gracilaria textorii; Red alga; Differential gene expression; GteCYP1A; GteHsp90; GteUB; Real-time quantitative PCR

Language	English

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