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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Analytical Science and Technology
Journal Basic Information
Journal DOI :
The Korean Society of Analytical Science
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Volume & Issues
Volume 27, Issue 6 - Dec 2014
Volume 27, Issue 5 - Oct 2014
Volume 27, Issue 4 - Sep 2014
Volume 27, Issue 3 - Jun 2014
Volume 27, Issue 2 - Apr 2014
Volume 27, Issue 1 - Feb 2014
Selecting the target year
Aptamer-based optical switch for biosensors
Lee, Joo-Woon ; Cho, Jeong Hwan ; Cho, Eun Jeong ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 121~139
DOI : 10.5806/AST.2014.27.3.121
In this review, we will discuss aptamer technologies including in vitro selection, signal transduction mechanisms, and designing aptamers and aptazyme for label-free biosensors and catalysts. Dye-displacement, a typical label-less method, is described here which allows avoiding relatively complex labeling steps and extending this application to any aptamers without specific conformational changes, in a more simple, sensitive and cost effective way. We will also describe most recent and advanced technologies of signaling aptamer and aptazyme for the various analytical and clinical applications. Quantum dot biosensor (QDB) is explained in detail covering designing and adaptations for multiplexed protein detection. Application to aptamer array utilizing self-assembled signaling aptamer DNA tile and the novel methods that can directly select smart aptamer or aptazyme experimentally and computationally will also be finally discussed, respectively.
Study of improving precision and accuracy by using an internal standard in post column isotope dilution method for HPLC-ICP/MS
Joo, Mingyu ; Park, Myungsun ; Pak, Yong-Nam ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 140~146
DOI : 10.5806/AST.2014.27.3.140
An internal standard was used in PCID (post column isotope dilution) to improve the accuracy and precision in quantification of various chemical species. The error occurring in the column was the largest in HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry) when PCID and other traditional quantification methods were compared with each other. Internal standard was effective in correcting the loss of sample in the column to improve accuracy and precision. When applied to SeMet, using MeSecys or
as an internal standard, relative errors were reduced from 31% and 13% to less than 1%, while standard deviations were reduced from 5.1% and 6.9% to 1.5% and 0.2%, respectively. Positive aspects of using an internal standard in PCID were compared with other quantitative techniques and discussed in detail.
Effect of forensic short wavelength UV on DNA
Kim, A-Ram ; Kim, Woo-Joong ; Park, Hee-Yeon ; Lim, Si-Keun ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 147~152
DOI : 10.5806/AST.2014.27.3.147
RUVIS(Reflective Ultraviolet Imaging System) is an effective equipment that detects the location of latent fingerprint at crime scene using short wavelength ultraviolet of 254 nm. In this study, the degree of DNA damage in biological samples was compared depending on the distance and time of processing using four commonly used RUVIS. 50% of DNA was damaged by treating 10 seconds at 10 cm distance in 3 types of RUVIS such as Police RUVIS, SIRCHIE mini light and SIRCHIE RUVIS. In addition, the degree of DNA damage was increased as the distance was closer and the treatment time was longer. It showed that short wavelength UV could cause DNA damage when used close to the samples at crime scene. Therefore, it was suggested to use RUVIS at a distance of at least 1 m. The degree of DNA damage was not significant by Polilight which used long wavelength ultraviolet of 350 nm. As a result, the choice and usage of which UV light and RUVIS were critical for detection of fingerprint and successful DNA typing.
A study on the quantitation of asbestos by the visual estimation and point counting method
Choi, Yun-Ho ; Kim, Tae-Hwa ; Bae, Yong-Soo ; Kim, Tae-Hyun ; Kim, Hyeon-Ja ; Jang, Eun-Ah ; Hwang, Beom-Goo ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 153~160
DOI : 10.5806/AST.2014.27.3.153
While variety of cases of studies about asbestos analysis methods are released internationally, the results of Asbestos Containing Materials (ACM) according to differences in the method of the analysis is becoming an issue. In this study, homogeneity ensured ACM samples were analyzed by visual estimation method and point counting method, and the result cound be used not only to improve the reliability on asbestos analysis of the institutions and analysts but also to obtain the basic data of Polarizing Light Microscope (PLM) analysis by comparing and evaluating. Asbestos analysis were divided into qualitative and quantitative analysis method. The quantitative analysis was performed by visual estimation method and point counting method (total 400 points) of EPA 600-R-93-116 method by using PLM. Firstly, The following was the result of homogeneity of the samples by ANOVA (Analysis of variance) and the results were satisfied. The results of qualitative analysis showed that the samples were chrysotile and amosite, and about the results of quantitative analysis, asbestos concentration determined by point counting method tend to be lower than concentrations determined by visual estimation method and also, pont counting method was a little more complicated and time-consuming.
Development of alternative assay method without hazardous reagent, carbon tetrachloride, for cefroxadine capsules in Korean Pharmaceutical Codex
Seo, Narae ; Kang, Yeoul ; Park, Yeong Shin ; Lee, Jong Min ; Jeong, Myung Jin ; Jeon, Sang Seol ; An, Jae Hyung ; Kim, Kyeong Ho ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 161~166
DOI : 10.5806/AST.2014.27.3.161
Currently oxidation-reduction titration method for the assay method using the hazardous reagent, carbon tetrachloride, for cefroxadine capsules has been used in Korean Pharmaceutical Codex. We developed and validated an alternative HPLC assay method by substituting the use of the hazardous reagent, carbon tetrachloride, to the use of less toxic ones like acetonitrile. The linearity of the calibration curves in the desired concentration range was good (
> 0.999). Relative standard deviations of intra-day precision were ranged between 0.30% and 0.69% and inter-day precision were ranged between 0.47% and 0.82%. Accuracy was obtained with recoveries in range of 100.20% and 100.56%. Developed method was applied to the determination of cefroxadine in commercial cefroxadine capsule and could be expected to become valuable tools for revising the Korean Pharmaceutical Codex.
Analytical method for combustible waste contaminated by the HF leakage from industrial process
Kang, Young-Yeul ; Kim, Yong-Jun ; Kim, Woo-Il ; Yoon, Cheol-Woo ; Yeon, Jin-Mo ; Shin, Sun-Kyoung ; Oh, Gil-Jong ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 167~171
DOI : 10.5806/AST.2014.27.3.167
Hydrofluoric acid (HF), a typical inorganic acid, has been used in the industry for its various usage and classified as the toxic compound, because it can cause the pneumonia and pulmonary edema when it was exposed to respiratory organs. The official environmental analytical method for fluorine and its compound in waste has not been developed. For this reason, we have faced some problem to treat the contaminated wastes by the HF leakage from industrial process. In this study, prepared for analytical method for combustible waste (crop, trees, etc.) generated from HF leaking accident and to be applied as the official analytical method for fluorine contaminated waste when the fluorine and its compound will be regulated as a hazardous material by the waste management law later.
Development and validation of an analytical method for fungicide fenpyrazamine determination in agricultural products by HPLC-UVD
Park, Hyejin ; Do, Jung-Ah ; Kwon, Ji-Eun ; Lee, Ji-Young ; Cho, Yoon-Jae ; Kim, Heejung ; Oh, Jae-Ho ; Rhee, Kyu-Sik ; Lee, Sang-Jae ; Chang, Moon-Ik ;
Analytical Science and Technology, volume 27, issue 3, 2014, Pages 172~180
DOI : 10.5806/AST.2014.27.3.172
Fenpyrazamine which is a pyrazole fungicide class for controlling gray mold, sclerotinia rot, and Monilinia in grapevines, stone fruit trees, and vegetables has been registered in republic of Korea in 2013 and the maximum residue limits of fenpyrazamine is set to grape, peach, and mandarin as 5.0, 2.0, and 2.0 mg/kg, respectively. Very reliable and sensitive analytical method for determination of fenpyrazamine residues is required for ensuring the food safety in agricultural products. Fenpyrazamine residues in samples were extracted with acetonitrile, partitioned with dichloromethane, and then purified with silica-SPE cartridge and eluted with hexane and acetone mixture. The purified samples were determined by HPLC-UVD and confirmed with LC-MS and quantified using external standard method. Linear range of fenpyrazamine was between
with the correlation coefficient (r) 0.999. The average recovery ranged from 71.8 to 102.7% at the spiked level of 0.05, 0.5, and 5.0 mg/kg, while the relative standard deviation was between 0.1 and 7.3%. In addition, limit of detection and limit of quantitation were 0.01 and 0.05 mg/L, respectively. The results revealed that the developed and validated analytical method is possible for fenpyrazamine determination in agricultural product samples and will be used as an official analytical method.