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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Analytical Science and Technology
Journal Basic Information
Journal DOI :
The Korean Society of Analytical Science
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Volume & Issues
Volume 27, Issue 6 - Dec 2014
Volume 27, Issue 5 - Oct 2014
Volume 27, Issue 4 - Sep 2014
Volume 27, Issue 3 - Jun 2014
Volume 27, Issue 2 - Apr 2014
Volume 27, Issue 1 - Feb 2014
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Analysis of the effects of δ-Aminolevulinic acid on the proliferation and apoptosis of mammalian cells
Jun, Yong-Woo ; Kim, Kun-Hyung ; Jo, Su-Yeon ; Lee, Jin-A ; Jang, Deok-Jin ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 223~227
DOI : 10.5806/AST.2014.27.5.223
-Aminolevulinic acid (ALA) is a compound which is widely present in the biosphere and plays an important role in the living body as an intermediate of the tetrapyrrole compound biosynthesis pathway that leads to heme in mammals and chlorophyll in plants. ALA is of interest as a biodegradable mediator, a growth regulator, a precursor of heme proteins, and an effective agent used in therapy of cancer. It has been recently reported that ALA is commonly used in dermatology, due to good effects of skin therapy. Although for the last few decades a substantial amount of research has been focused on the elucidation of the mechanism of ALA and the improvement of its therapeutic activity, it`s effect on the cell functions and growth was not cleared. Here, we identified that ALA treatment could attenuate cell proliferation of HEK293T and HaCaT cells. In addition, ALA treatement could induce apoptosis of HeLa cells. These results suggest that apoptosis induced by ALA treatment might be responsible for inhibition of cell proliferation. These results propose the possibility of the improved therapeutic strategy making ALA one of the effective drugs used in human cancers.
Fabrication and characterization of block copolymer (PCL/PCL-PEG) nanofibers binding with collagen by electrospun
Lee, Jin Woo ; Yoon, Kuk Ro ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 228~233
DOI : 10.5806/AST.2014.27.5.228
Electrospun polymeric nanofibers have been extensively studied for biomedical materials because of their unique structures and relatively easy fabrication with biocompatible polymers. The amount of surface exposed amine groups increases as the blend ratio of block copolymer increases. Cell attachments on the nanofibers change according to the ratio of the block copolymer ((Poly(e-caprolactone, PCL), Poly(e-caprolactone)-Poly (ethylen glycol-
)) in the blend. We assume that the PEG and amine moiety plays a significant role in biocompatibility of nanofiber surfaces. Collagen was used as a grafting material on the composite nanofibers to enhance the cell adhesion because the collagen is a major constituent of connective tissue.
Development and validation of an analytical method for nematicide imicyafos determination in agricultural products by HPLC-UVD
Do, Jung-Ah ; Park, Hyejin ; Kwon, Ji-Eun ; Choi, Won-Jo ; Lee, Hyun-Sook ; Chang, Moon-Ik ; Hong, Jin-Hwan ; Oh, Jae-Ho ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 234~242
DOI : 10.5806/AST.2014.27.5.234
Imicyafos which is a nematicide for controlling root-knot nematodes has been registered in the Republic of Korea in 2012, and the maximum residue limits of imicyafos are set to watermelon and korean melon as each 0.05 mg/kg. Extremely reliable and sensitive analytical method is required for ensuring food safety on imicyafos residues in agricultural commodities. Imicyafos residues in samples were extracted with acetone, partitioned with hexane and dichloromethane, and then purified with florisil. The purified samples were analyzed by HPLC-UVD and confirmed with LC-MS. Linear range was between 0.1~5 mg/kg with the correlation coefficient (
) 0.99997. Average recoveries of imicyafos ranged from 77.0 to 115.4% at the spiked levels of 0.02 and 0.05 mg/kg with the relative standard deviations of 2.2~9.6%. Limit of detection and quantification were 0.005 and 0.02 mg/kg, respectively. An inter-laboratory study was conducted to validate the determination method in depth, and the results were satisfactory. All of the validation results revealed that the developed analytical method in this study is relevant for imicyafos determination in agricultural commodities and will be used as an official analytical method.
Identification of process generating formaldehyde in a furniture manufacturer
Yoo, Kye-Mook ; Lee, Mi-Young ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 243~247
DOI : 10.5806/AST.2014.27.5.243
Formaldehyde is defined as carcinogen causing leukaemia, lymphoma or nasopharyngeal carcinoma at high level of exposure. Furniture-manufacturing workers can be exposed to formaldehyde, which implies serious impact on health of the workers. The authors carried out ambient monitoring of formaldehyde in the field, and identified the source of formaldehyde generated during the working process by testing the condition in the laboratory settings. After sampling formaldehyde in the air with 2,4-DNPH (2,4-dinitrophenylhydrazine) coated silica gel, we extracted formaldehyde derivative with acetonitrile and analyzed the extract using HPLC with UV detector at 360 nm. Formaldehyde was separated by ACQUITY UPLC BEH
column at a flow rate of 0.5 mL/min using 45% acetonitrile as mobile phase. The workers were exposed to higher level of formaldehyde than normal air. Formaldehyde up to 0.31 ppm was detected in the process of veneer attachment, which exceeded 0.3 ppm, the ceiling value of ACGIH standard. The laboratory test of measuring formaldehyde generated from the glue and veneer used in the attachment process resulted in more formaldehyde generation as the temperature increased, and more from the veneer. Heating the veneer to
following the real condition of the manufacturing site generated 1.14-2.70 ppm of formaldehyde from the sample, which was 2-5 times higher level than Korean limit of exposure (0.5 ppm). As the workers handling and processing the veneer which was produced by wet process had high possibility to be exposed to formaldehyde, urgent improvement and management of working environment of furniture manufacturer is demanded.
Effects of low-level exposure to manganese and lead on immune function
Kim, Ki-Woong ; Park, SangHwoi ; Won, Yong Lim ; Lee, Sung Kwang ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 248~253
DOI : 10.5806/AST.2014.27.5.248
This study aimed to evaluate the effects of exposure to manganese (Mn) and lead (Pb) on immune system. The subjects were 42 male workers, among whom 13 office workers (Group I) had never been occupationally exposed to heavy metals, 21 were worked in manufacturing factories (Group II) and 8 were welders (Group III). The mean blood Mn and Pb level by groups were significantly different. The numbers of CD19+ and total lymphocytes in Group I were significantly higher than those in other groups, but no significant differences were found in other T lymphocytes subpopulation. Mn and Pb concentrations showed negative correlation with T lymphocytes subpopulation, but Mn concentrations were statistical significances with T lymphocytes subpoplation except CD4+CD45RO+ and natural killer cell. Pb concentration was only statistical significance with total lymphocytes. Our results suggest that occupationally exposed to Mn and Pb can affect the cellular immune response.
Total content characteristics of inorganic and organic substances from wastes from thermal processes
Yeon, Jin-Mo ; Kim, Woo-Il ; Kang, Young-Yeul ; Jeon, Tae-Wan ; Jeong, Seong-Kyeong ; Cho, Yoon-A ; Kim, Min-Sun ; Shin, Sun-Kyoung ; Oh, Gil-Jong ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 254~260
DOI : 10.5806/AST.2014.27.5.254
In this study, heavy metals, PCDD/PCDFs, PAHs in wastes generated from thermal processes were analyzed. Waste from lead thermal metalurgy (EWC 10 04) inorganic metal substances in the regulation were detected in the highest concentrations of Pb. EWC 10 04 seems to be a result of the dust. Waste from zinc thermal metalurgy (EWC 10 05) inorganic metal substances in the regulation were detected in high concentration of Zn. EWC 10 05 seems to be a result of the dust. Waste from copper thermal metalurgy (EWC 10 06) Cu in the 651,77 mg/kg to 651 times higher than regulation standard appeared in the copper thermal metallurgy process seems to be a result of dust. The concentrations of PCDD/PCDFs ranged from 0.0005~11.748 ng-TEQ/g in dust, 0.0027 ng-TEQ/g in fly ash. PCDD/PCDFs content was not detected in excessive value in regulation standard. PAHs concentration was in the range of ND~118.9 mg/kg in Naphthalene, ND~9.6 mg/kg in Phenanthrene, ND~48.4 mg/kg in Benzo[b]fluoranthene, ND~62.6 mg/kg in Benzo[a]pyrene, ND~10.7 mg/kg in Fluoranthene, ND~11.5 mg/kg in Benzo[a]anthracene.
Study on the methods of risk assessment of human exposure by using of PVC flooring
Kim, Woo Il ; Cho, Yoon A ; Kim, Min Sun ; Lee, Ji Youmg ; Kang, Young Yeul ; Shin, Sun Kyoung ; Jeong, Seong Kyoung ; Yeon, Jin Mo ;
Analytical Science and Technology, volume 27, issue 5, 2014, Pages 261~268
DOI : 10.5806/AST.2014.27.5.261
In advanced countries, a variety of consumer exposure assessment models including CONSEXPO, are developed to manage risks of consumer products containing hazardous materials. The models are used to assess the risks of exposure to hazardous chemicals in consumer products, which serves as a foundation for regulation standards. In this study, exposure assessment models applicable for various scenarios were reviewed and a proper model was applied for the selected products and risk assessment was conducted at each stage to establish a risk assessment procedure for different types of products. Based on the exposure scenario, exposure factor was selected and according to the algorithm produced based on CONSEXPO exposure model, some level of phthalates were detected from some types of PVC flooring. However, a correlation between phthalate content and migration rate showed r-square 0.0065, little correlation, which is inadequate for estimating standard value. For this reason, it seems valid that the current standard be in place. Additionally, any new standard was not suggested as VOCs were not found harmful to human health, allowing the existing standard to be continuously applied. No migration rate was found for heavy metals and risk assessment was not performed accordingly. In this aspect, it is presumed that hazards to health through dermal exposure would be very little.