Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Analytical Science and Technology
Journal Basic Information
Journal DOI :
The Korean Society of Analytical Science
Editor in Chief :
Volume & Issues
Volume 28, Issue 6 - Dec 2015
Volume 28, Issue 5 - Oct 2015
Volume 28, Issue 4 - Aug 2015
Volume 28, Issue 3 - Jun 2015
Volume 28, Issue 2 - Apr 2015
Volume 28, Issue 1 - Feb 2015
Selecting the target year
Regulatory mechanism of Angelica Gigas extract powder on matrix metalloproteinases in vitro and in vivo model
Kwon, Jin-Hwan ; Han, Min-Seok ; Lee, Yong-Moon ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 361~369
DOI : 10.5806/AST.2015.28.6.361
The precise mechanism underlying the therapeutic efficacy of an extraction powder of Angelica gigas (AGE) for the treatment of degenerative osteoarthritis was investigated in primary cultured rabbit chondrocytes and in a monosodium-iodoacetate (MIA)-induced osteoarthritis rat model. The treatment with AGE (50 μg/mL) effectively inhibited NF-B activation. The anti-inflammatory mechanism was clarified by gelatin zymography and western blotting measurements of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities. The AGE (50 μg/mL) treatment significantly reduced MMP-9 activity. The constituents of AGE— decursinol, decursin, and decursinol angelate—were determined by LC-MS/MS after a 24 hr treatment of rabbit chondrocytes. The contents of the major products, decursin and decursinol angelate, were 3.62±0.47 and 2.14 ±0.36 μg/mg protein, respectively in AGE-treated (50 μg/mL) rabbit chondrocytes. An in vivo animal study on rats fed a diet containing 25, 50, and 100 mg/kg AGE for 3 weeks revealed a significant inhibition of the MMPs in the MIA-induced rat articular cartilage. The genetic expression of arthritic factors in the articular cartilage was examined by RT-PCR of collagen Type I, collagen Type II, aggrecan, and MMP (MMP3, MMP-9, MMP13). Specifically, AGE up-regulated the expression of collagen Type I, collagen Type II, and aggrecan and inhibited MMP levels at all tested concentrations. Collectively, AGE showed a strong specific site of action on MMP regulation and protected against the degeneration of articular cartilage via cellular regulation of MMP expression both in vitro and in vivo.
Determination of phenol using solid-phase extraction and HPLC/MSD/FLD in water
Lee, Taejoon ; Park, Keun-Young ; Pyo, Dongjin ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 370~376
DOI : 10.5806/AST.2015.28.6.370
An analytical method for determining phenol considered priority pollutants of the US EPA and precursor of toxic phenolic compounds by solid-phase extraction (SPE) and high performance liquid chromatographic systems (HPLC) equipped with fluorescence and mass selective detectors have been developed. The SPE process for sample preconcentration was performed on a commercially available Oasis HLB cartridge packed with polymeric sorbents. The effect of pH, elution solvent, and elution volume on the recoveries of the analytes were investigated with HPLC/FLD. Average recovery of >87.0% was achieved with 60 mg sorbents using 5 mL of methanol as an elution solvent at pH=3.
Status of a national monitoring program for environmental radioactivity and investigation of artificial radionuclide concentrations (
I) in rivers and lakes
Kim, Jiyu ; Jung, Hyun-ji ; An, Mijeong ; Hong, Jung-Ki ; Kang, Taegu ; Kang, Tae-Woo ; Cho, Yoon-Hae ; Han, Yeong-Un ; Seol, Bitna ; Kim, Wansuk ; Kim, Kyunghyun ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 377~384
DOI : 10.5806/AST.2015.28.6.377
A survey of the artificial radionuclides in rivers and lakes was conducted to investigate their levels in surface water. Water samples were collected at 60 points and analyzed by gamma-ray spectrometry with a measurement time of 10,000 seconds for
I. The obained values were lower than MDA for all points, except one point for
I that was 0.533±0.058 Bq/L.
I is known as a radioactive material that occurs frequently in sewage treatment plants. Because it is often used for medical treatments and subject to spreading into the environment due to the excretion from the patients. For the point where
I was detected, we conducted additional investigation on the upstream river point and the effluent points of nearby sewage treatment plant to find the source of
I was not detected at the upstream points of one of the upstream sewage treatment plants but found at the downstream points with the level being 0.257±0.034 to 0.799±0.051 Bq/L, proving the sewage treatment plant was the
Establishment of analytical methods for HPHC list of mainstream cigarette smoke
Park, Hyoung-Joon ; Lee, Jin-Hee ; Cho, So-Hyun ; Heo, Seok ; Yoon, Chang-yong ; Baek, Sun-Young ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 385~397
DOI : 10.5806/AST.2015.28.6.385
Harmful and potentially harmful constituents (HPHCs) are chemical compounds in tobacco smoke that cause harm to smokers and non-smokers. This study established and validated methods for the analysis of HPHCs from mainstream cigarette smoke. The analyzed HPHCs were categorized into seven groups: aromatic amines, volatile organic compounds (VOCs), heavy metals, tobacco specific nitrosamines (TSNAs), benzo[a]pyrene (B[a]P), ammonia, and carbonyl compounds. The methods were validated by specificity, linearity, limit of detection (LOD), accuracy, precision, and recovery. These validated methods were then applied to the reference cigarettes (1R5F, 3R4F). The correlation coefficients (r
) for the calibration curves of the seven groups were over 0.995. The LODs showed values of 0.01-0.04 ng/cig cig for aromatic amines, 0.01-0.16 μg/cig for VOCs, 0.01-1.27 ng/cig for heavy metals, 0.06-0.28 ng/cig for TSNAs, 0.04 ng/cig for benzo[a]pyrene, 0.08 μg/cig for ammonia, and 0.78-1.77 μg/cig for carbonyl compounds. The precisions obtained from the intra and inter-day batches were less than 15%. The accuracy and the recovery range were less than 15% and 79.2-117.5%, respectively. The proposed methods can therefore be applied for determining HPHCs in tobacco mainstream smoke.
A case study of verifying a suicide by carbon monoxide intoxication committed by burning an ignition charcoal briquette
Sung, Tae-myung ; Jo, Ju-ik ; Ahn, Phil-sang ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 398~408
DOI : 10.5806/AST.2015.28.6.398
Carbon monoxide (CO) intoxication, arising from CO from an ignited charcoal briquette (ICB), is a popular means of committing suicide in Korea. Most CO intoxications are related to suicide attempts; however, the possibility of a homicide disguised as a suicide cannot be ruled out. Therefore, forensic investigation of the deceased and the crime scene is crucial to confirm that the deceased committed suicide. Detection of the components of an ICB on the objects suspected of being contacted by the deceased, such as the hands, nostrils, and doorknobs, is essential for linking the crime scene to the victim in the case of suicides by ignited ICBs. The traces from an ICB were analyzed by investigating the morphological characteristics and obtaining elemental compositions. The ICBs were completely different from blackened wood, as detected by discriminant analysis with the elements of carbon and oxygen. We analyzed one case of CO intoxication to demonstrate an excellent procedure for verifying whether a suicide occurred with an ICB. We employed SEM-EDX for the analysis of an ICB, microscope-FT/IR and pyrolysis-GC/MS for a partly burnt resin-type substance, GC/MS for diphenhydramine (a sleeping drug), and GC/TCD for the CO-Hb level. We detected traces of an ICB on the hands, nostrils, and doorknobs, which were all discriminated into an ICB group. Detection of ICB traces from the nostrils could indicate that the deceased started the fire themselves to commit suicide. The partially burnt black material was analyzed as an acrylronitrilestyrene polymer, which is normally used to make bags for carrying or wrapping and could be assumed to have been used to transport the ICB. Diphenhydramine, a sleeping drug, was detected at a level of 2.3 mg/L in the blood, which was lower than that in fatal cases (8-31 mg/L; mean 16 mg/L). A CO-Hb level of 79% was found in the blood, which means that the cause of death was CO intoxication. The steps shown here could represent an ideal method for reaching a verdict of suicide by CO intoxication produced by burning an ICB in a sealed room or a car.
Improvement of analytical methods for arsenic in soil using ICP-AES
Lee, Hong-gil ; Kim, Ji In ; Kim, Rog-young ; Ko, Hyungwook ; Kim, Tae Seung ; Yoon, Jeong Ki ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 409~416
DOI : 10.5806/AST.2015.28.6.409
ICP-AES has been used in many laboratories due to the advantages of wide calibration range and multi-element analysis, but it may give erroneous results and suffer from spectral interference due to the large number of emission lines associated with each element. In this study, certified reference materials (CRMs) and field samples were analyzed by ICP-AES and HG-AAS according to the official Korean testing method for soil pollution to investigate analytical problems. The applicability of HG-ICP-AES was also tested as an alternative method. HG-AAS showed good accuracies (90.8~106.3%) in all CRMs, while ICP-AES deviated from the desired range in CRMs with low arsenic and high Fe/Al. The accuracy in CRM030 was estimated as below 39% at the wavelength of 193.696 nm by ICP-AES. Significant partial overlaps and sloping background interferences were observed near to 193.696 nm with the presence of 50 mg/L Fe and Al. Most CRMs were quantified with few or no interferences of Fe and Al at 188.980 nm. ICP-AES properly assessed low and high level arsenic for field samples, at 188.980 nm and 193.696 nm, respectively. The importance of the choice of measurement wavelengths corresponding to relative arsenic level should be noted. Because interferences were affected by the sample matrix, operation conditions and instrument figures, the analysts were required to consider spectral interferences and compare the analytical performance of the recommended wavelengths. HG-ICP-AES was evaluated as a suitable alternative method for ICP-AES due to improvement of the detection limit, wide calibration ranges, and reduced spectral interferences by HG.
A study of relationship between stomach cancer and selenoproteins in Korean human blood serum
Park, Myungsun ; Pak, Yong-Nam ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 417~424
DOI : 10.5806/AST.2015.28.6.417
In this study, the relationship between selenoprotein concentrations in blood and stomach cancer have been searched for Korean. The concentration of each selenoprotein in blood serum was analyzed and the correlation between the concentration and stomach cancer was studied to find a potential for using Selenium as a biomarker. In concentration determination, a simple calibration curve method was used with the monitoring of m/z 78 without the use of solid phase extraction. This is a lot more simple than the method using SPE with post column isotope dilution. The result obtained from the analysis of CRM BCR-637, 72.20±3.35 ng·g
, showed similar value of reference value (81±7 ng·g
). The total concentration of Se for the controlled group, cardiovascular patients group, was 105.70±21.20 ng·g
. This value was the same as normal healthy person reported earlier. Each selenoprotein concentration of GPx, SelP and SeAlb was 26.12±7.84, 65.15±14.50, 14.43±6.99 ng·g
, respectively. The distribution of each selenoprotein was 24.7%, 61.6%, and 13.7%, which was similar to the normal person. The result of stomach cancer patients, the total concentration of Se was 76.11±28.12 ng·g
and each concentration of GPx, SelP and SeAlb was 15.41±9.01, 50.83±17.91, and 9.87±5.21 ng·g
, respectively. The total and each selenoprotein concentration level showed significant decrease for the stomach cancer patients. The level of decrease was 41.0% for GPx, 22.0% for SelP, and 31.6% for SeAlb. However, the distribution of each selenoprotein was not much different. Either total Selenium or each selenoprotein could be used as a possible index for the diagnosis of cancer. However, in age group study, it is shown that young age group (30's-40's) did not show much difference.
Development of a cholesterol biosensor modified with carbon nanotube
Kim, Haidong ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 425~429
DOI : 10.5806/AST.2015.28.6.425
A cholesterol biosensor was developed using a modified carbon electrode with carbon nanotubes. The disposable cholesterol biosensor was modified with carbon nanotubes to enhance electron transfer during the enzymatic reaction of cholesterol. Cholesterol oxidase and peroxidase, with potassium ferrocyanide as a mediator, were immobilized on a screen-printed carbon nanotube electrode. The electrochemical cholesterol biosensor developed using carbon nanotubes showed a rapid and reliable signal for measuring total cholesterol. The cholesterol sensor showed a linear response in 5 seconds with a small volume (0.5 μL) in the range of 100~400 mg/dL, with a coefficient of variation of 4.0%.
Two dimensional analysis between the performance and the sensitivity of methylnitroimidazole derivatives
Rim, One Kwon ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 430~435
DOI : 10.5806/AST.2015.28.6.430
Two-dimensional analysis between the explosive performance and the impact sensitivity for methylnitroimidazole derivatives was performed to understand where these new energetic molecules could be utilized. The explosive performance was analyzed with the Cheetah program, while the impact sensitivity was predicted using neural network analysis. Successive nitration of methylimidazole made the molecule more sensitive, but methyltrinitroimidzole appeared to have a relatively good safety characteristic. We recently developed a novel method to analyze the potential usage of new energetic molecules using a two-dimensional chart, where the explosive performance and the impact sensitivity were located on the X-axis and Y-axis, respectively. An analysis of a two-dimensional plot between the performance and the sensitivity indicated that methyldinitroimidazole would be useful for insensitive explosive formulations, while methyltrinitroimidazole was forecasted for use as an ingredient for high explosive formulations.
Analysis of volatile compounds and metals in essential oil and solvent extracts of Amomi Fructus
Lee, Sam-Keun ; Eum, Chul Hun ; Son, Chang-Gue ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 436~445
DOI : 10.5806/AST.2015.28.6.436
Amomi Fructus with anti-oxidative activity was chosen and essential oil was obtained by SDE (simultaneous distillation extraction), and 39 constituents were determined by GC-MS (gas chromatography-mass spectrometry). Major components were camphor, borneol acetate, borneol, D-limonene and camphene. Three solvent extracts such as hexanes, diethyl ether and methylene chloride from Amomi Fructus were obtained. These were analyzed by GC-MS and 4 more constituents were identified in addition to 39 components discovered in essential oil. Five major components such as camphor, borneol acetate, borneol, D-limonene and camphene were also detected, however the relative peak percents of those components were different from those of constituents in essential oil. To estimate the kind and the amount of materials evaporated at certain temperature and conditions from essential oil and solvent extracts, dynamic headspace apparatus was used and materials evaporated and trapped at certain conditions were analyzed by GC-MS. Recovery yield of SDE method from Amomi Fructus was measured by using camphor and standard calibration solution of camphor methanol solution and, the yield was 82.0%. Content of Hg was measured by mercury analyzer and contents of Cd, Pb, Cr, Mn, Co, Ni, Cu and Zn in Amomi Fructus, essential oils and solvent extracts were determined by ICP-MS (Inductively coupled plasma-mass spectrometer). Pb, Cd and Hg were measured in the concentration of 0.72 mg/kg, <0.10 mg/kg and 0.0023 mg/kg, respectively and these were below permission level of purity test. Contents of Mn, Cu and Zn in Amomi Fructus were 213 mg/kg, 8.29 mg/kg and 31.0 mg/kg, respectively and which were relatively higher than other metals such as Cr, Co and Ni. Metals such as Mn (0.65 ~ 9.08 mg/kg), Cu (1.16 ~ 4.40 mg/kg) and Zn (1.10 ~ 3.80 mg/kg) in essential oil and solvent extracts were detected. At this point it is not clear that the metals were cross-contaminated in the course of treating Amomi Fructus or metals were contained in Amomi Fructus. The influence evaluation toward biological model study of these metals in essential oil and solvent extracts will be needed.
Evaluation of proficiency and improvement of accuracy on the analysis of brominated flame retardants (PBDEs) in ABS polymer
Ryu, Jehoon ; Kim, Dalho ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 446~452
DOI : 10.5806/AST.2015.28.6.446
In order to evaluate and improve the ability of Korean testing laboratories to measure Polybrominated diphenyl ethers in acrylonitrile-butadiene-styrene (ABS), a proficiency test was organised by Korea Research Institute of Standards and Science (KRISS) based on ISO/IEC 17043. The proficiency test material used was 10 g of a granular ABS fortified with a mixture of congeners of PBDE (BDE-154, 183, 206, 209). Homogeneity and stability were investigated to assess the adequacy of the test material. The certified value established by KRISS based on the national reference was used for assigned value of each PBDE. The test materials were distributed to the 16 participating laboratories. The participating laboratories were requested to analyse the samples employing the methods used in their routine analysis. Each laboratory was given it’s own code to secure the anonymity. Participants results were evaluated with z-scores according to ISO/IEC 17043. The standard deviation for proficiency assessment was set by standard deviation of the participants results except for outlier. The results, the laboratory's performance and improvement of accuracy were discussed.
Optimization of fractionation efficiency (FE) and throughput (TP) in a large scale splitter less full-feed depletion SPLITT fractionation (Large scale FFD-SF)
Eum, Chul Hun ; Noh, Ahrahm ; Choi, Jaeyeong ; Yoo, Yeongsuk ; Kim, Woon Jung ; Lee, Seungho ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 453~459
DOI : 10.5806/AST.2015.28.6.453
Split-flow thin cell fractionation (SPLITT fractionation, SF) is a particle separation technique that allows continuous (and thus a preparative scale) separation into two subpopulations based on the particle size or the density. In SF, there are two basic performance parameters. One is the throughput (TP), which was defined as the amount of sample that can be processed in a unit time period. Another is the fractionation efficiency (FE), which was defined as the number % of particles that have the size predicted by theory. Full-feed depletion mode (FFD-SF) have only one inlet for the sample feed, and the channel is equipped with a flow stream splitter only at the outlet in SF mode. In conventional FFD-mode, it was difficult to extend channel due to splitter in channel. So, we use large scale splitter-less FFD-SF to increase TP from increase channel scale. In this study, a FFD-SF channel was developed for a large-scale fractionation, which has no flow stream splitters (‘splitter less’), and then was tested for optimum TP and FE by varying the sample concentration and the flow rates at the inlet and outlet of the channel. Polyurethane (PU) latex beads having two different size distribution (about 3~7 µm, and about 2~30 µm) were used for the test. The sample concentration was varied from 0.2 to 0.8% (wt/vol). The channel flow rate was varied from 70, 100, 120 and 160 mL/min. The fractionated particles were monitored by optical microscopy (OM). The sample recovery was determined by collecting the particles on a 0.1 µm membrane filter. Accumulation of relatively large micron sized particles in channel could be prevented by feeding carrier liquid. It was found that, in order to achieve effective TP, the concentration of sample should be at higher than 0.4%.
Quantitative analysis of cholesterol in infant formula by isotope dilution liquid chromatography-tandem mass spectrometry
Ahn, Eun Jeong ; Lee, Hwa Shim ; Kim, Byung Joo ; Lee, Gae Ho ;
Analytical Science and Technology, volume 28, issue 6, 2015, Pages 460~466
DOI : 10.5806/AST.2015.28.6.460
An isotope dilution liquid chromatography tandem mass spectrometry was developed as a primary method for the quantitative analysis of cholesterol in infant formula. Cholesterol-d
was used as an internal standard and spiked into the infant formula sample. In order to release cholesterol out of cholesteryl ester, which is cholesterol bound to fatty acids in infant formula, saponification was carried out. Saponification conditions were optimized with heating temperature, reaction time and the concentration of KOH. The optimum conditions were as follows; heating temperature was 70 ℃, reaction time was 180 min and the concentration of KOH was 0.8 mL of 8 M KOH for about 0.1 g infant formula sample. Extraction of cholesterol out of sample solution was carried out with hexane uisng liquid-liquid extraction. Chromatographic analysis was carried out using Phenomenex Kinetex C
column. Mobile phase was 0.1% acetic acid in methanol/water (v/v, 99/1) and flow rate was 0.3 mL/min. Cholesterol and cholesterol-d
were monitored at mass transfer m/z 369/259 and 373/263 respectively. Reproducibility of the method was evaluated to be 0.23% of the measurement result. The expanded uncertainty of the measurement result of cholesterol in infant formula was approximately 1.9% at a 95% confidence level. NIST standard reference material having certified values of cholesterol in infant formula, was analyzed in order to verify this method. The ID-LC/MS/MS results were well agreed with the certified values of NIST SRM within the uncertainty.