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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Analytical Science and Technology
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Journal DOI :
The Korean Society of Analytical Science
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Volume & Issues
Volume 29, Issue 3 - Jun 2016
Volume 29, Issue 2 - Apr 2016
Volume 29, Issue 1 - Feb 2016
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Effect of functional group on activity and stability of lipase immobilized on silica-coated magnetite nanoparticles with different functional group
Lee, Hye Rin ; Kim, Moon Il ; Hong, Sang Eun ; Choi, Jaeyeong ; Kim, Young Min ; Yoon, Kuk Ro ; Lee, Seungho ; Ha, Sung Ho ;
Analytical Science and Technology, volume 29, issue 3, 2016, Pages 105~113
DOI : 10.5806/AST.2016.29.3.105
The present study investigated the immobilization of lipases on silica nanoparticles and silica-coated magnetite nanoparticles as supports with a functional group to enhance the stability of lipase. The influence of functional groups, such as the epoxy group and the amine group, on the activity and stability of immobilized lipase was also studied. The epoxy group and the amino group were introduced onto the surface of nanoparticles by glycidyl methacrylate and aminopropyl triethoxysilane, respectively. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles with a functional group showed slightly lower initial enzyme activities than free enzyme; however, the immobilized Candida rugosa lipase retained over 92 % of the initial activity, even after 3 times reuse. Lipase was also immobilized on the silica-coated magnetite nanoparticles by cross-linked enzyme aggregate (CLEA) using glutaraldehyde and covalent binding, respectively, were also studied. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles by CLEA and covalent binding showed higher enzyme activities than free enzyme, while immobilized Candida rugosa lipase retained over 73 % of the initial activity after 5 times reuse.
Study on characteristics of specific hazardous substances in the industrial wastewater effluent
Kim, Seungho ; Choi, Youngseop ; Kim, Yunhee ; Kim, Jongmin ; Chang, Gilsik ; Bae, Seokjin ; Cho, Younggwan ;
Analytical Science and Technology, volume 29, issue 3, 2016, Pages 114~125
DOI : 10.5806/AST.2016.29.3.114
In this study, 165 wastewater discharge facilities in 10 business types were investigated with regard to 24 specific hazardous substances that included heavy metals, VOCs, CN, and phenol in the Gwangju city. Cu in the range from from 0.008 to 35.420 mg/L was detected in all business types and the detection rate was 46.8 %. Other heavy metals, such as Cd, As, Hg, Pb, and Cr
were detected as well. However, their detection rates ranged between 0.6 and 1.8 %. CN and phenol were detected in one and five facilities, respectively. 12 species of VOCs were detected: chloroform 80.6 % (0.42 to 81.60 μg/L), benzene 16.4 % (1.49 to 3.31 μg/L), trichloroethylene 11.5 % (1.78 to 6.02 μg/L), 1,1-dichloroethylene 10.3 % (1.23 to 5.89 μg/L), and dichloromethane 8.5 % (0.28 to 968.86 μg/L) in the detection rate order. The concentration of VOCs was detected in trace amounts, except for dichloromethane that exceeded the effluent quality standard in three business types, namely, metal manufacturing, food industry, and car washing facility. Chloroform was detected in all business types, where 24.88 μg/L were detected in the laundry business and 53.41 μg/L in the water supply business; the mean concentration of chloroform in these two business types was higher than elsewhere. Therefore, for the disposal of non-degradable specific hazardous substances in industrial wastewater, it is necessary to introduce physical and chemical processes, such as activated carbon adsorption, fenton oxidation, ozone treatment, as well as photocatalyst and the UV radiation.
Evaluation on the removal efficiency of pharmaceutical compounds in conventional drinking water treatment processes
Seo, Hee-Jeong ; Park, Yong-Hoon ; Kang, In-Sook ; Myong, Hwa-Bong ; Song, Yang-Suk ; Kang, Yeong-Ju ;
Analytical Science and Technology, volume 29, issue 3, 2016, Pages 126~135
DOI : 10.5806/AST.2016.29.3.126
In the present study, we evaluated the efficiency of the drinking water treatment unit processes controlled by targeting high pharmaceutical compounds that are likely to be released into the water supply. In the coagulation process, the removal rate of sulfonamide, an antibiotic, amounted to 22.6~42.1 %, that of naproxen to 28.2 %, and that of acetaminophen to 20 %. Trimethoprim has demonstrated a low removal rate (4.4 %), while the removal rate of erythromycin was 2.4 %; aspirin was not removed at all. When applying a mixture of chlorination and the coagulation process, the removal rate was increased with increasing the chlorine dosage. When the chlorine injection with the concentration of 3 mg/L was applied, sulfonamide antibiotics, acetaminophen and naproxen, were completely removed. Trimethoprim exhibited a high removal efficiency of ca. 98%, while the removal efficiency of erythromycin was about 55 %; at the same time, aspirin showed a lower removal ratio (ca. 10 %). When applying the powdered activated carbon adsorption process, the removal rate was increased with increase of the concentration of the powder activated carbon injection. Sulfonamide antibiotics showed about 18~50 % removal efficiency in the 1 mg/L, the removal rate was increased by at least 80 % in 25 mg/L. The evaluation results of the titration injection concentration of chlorine treatment and adsorption, coagulation process for the efficient processing of the remaining pharmaceutical compounds in the water treatment process, when applying the chlorine 3 mg/L, powdered activated carbon 10 mg/L and coagulant 15 mg/L were removed more than 90 %.
A study of the distribution of glass particles on patrol car seats
Kim, Mihye ; Ko, Gangseok ; Kim, Sookyung ; Hong, Sungwook ;
Analytical Science and Technology, volume 29, issue 3, 2016, Pages 136~141
DOI : 10.5806/AST.2016.29.3.136
In the present study, the distribution of glass particles inside 10 patrol cars of Chungnam Province has been studied. The target seats were front seats (driver’s seat, passenger’s seat) and the back seat. The target areas were the bottom of the seat (seat contacting buttocks, back of the seat, and the corner of the seat (the bottom and back attached part)). The target areas were tape lifted with an adhesive tape. The glass particles adhering to the adhesive tape were examined and counted under a stereomicroscope. The total number of glass particles found was 679. Among them, 471 (driver’s seat 293, passenger seat 178) were collected from front seats, which are usually occupied by police officers. The majority of glass particles were under 0.49 mm size. The results show that the majority of glass particles can be found on the front seats, rather than on the back seat. There is a high probability that glass particles found on the front seat adhere to police officers, so that to get further transferred to the convict upon physical contact (secondary transfer). Thus, there is a risk of misinterpretation of the value of glass evidence in the course of forensic examinations. Hence, a separate method to prevent cross contamination has to be prepared by police authorities as soon as possible.
A preliminary study and its application for the development of the quantitative evaluation method of developed fingerprints on porous surfaces using densitometric image analysis
Cho, Jae-Hyun ; Kim, Hyo-Won ; Kim, Min-Sun ; Choi, Sung-Woon ;
Analytical Science and Technology, volume 29, issue 3, 2016, Pages 142~153
DOI : 10.5806/AST.2016.29.3.142
In crime scene investigation, fingerprint identification is regarded to be one of the most important techniques for personal identification. However, objective and unbiased evaluation methods that would compare the fingerprints with diverse available and developing methods are currently lacking. To develop an objective and quantitative method to improve fingerprint evaluation, a preliminary study was performed to extract useful research information from the analysis with densitometric image analysis (CP Atlas 2.0) and the Automated Fingerprint Identification System (AFIS) for the developed fingerprints on porous surfaces. First, inked fingerprints obtained by varying pressure (kg.f) and pressing time (sec.) to find optimal conditions for obtaining fingerprint samples were analyzed, because they could provide fingerprints of a relatively uniform quality. The extracted number of minutiae from the analysis with AFIS was compared with the calculated areas of friction ridge peaks from the image analysis. Inked fingerprints with a pressing pressure of 1.0 kg.f for 5 seconds provided the most visually clear fingerprints, the highest number of minutiae points, and the largest average area of the peaks of the friction ridge. In addition, the images of the developed latent fingerprints on thermal paper with the iodine fuming method were analyzed. Fingerprinting condition of 1.0 kg.f/5 sec was also found to be optimal when generating highest minutiae number and the largest average area of peaks of ridges. Additionally, when the concentration of ninhydrin solution (0.5 % vs. 5 %) was used to compare the developed latent fingerprints on print paper, the best fingerprinting condition was 2.0 kg.f/5 sec and 5 % of ninhydrin concentration. It was confirmed that the larger the average area of the peaks generated by the image analysis, the higher the number of minutiae points was found. With additional tests for fingerprint evaluation using the densitometric image analysis, this method can prove to be a new quantitative and objective assessment method for fingerprint development.