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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Analytical Science and Technology
Journal Basic Information
Journal DOI :
The Korean Society of Analytical Science
Editor in Chief :
Volume & Issues
Volume 8, Issue 4 - 00 1995
Volume 8, Issue 3 - 00 1995
Volume 8, Issue 2 - 00 1995
Volume 8, Issue 1 - 00 1995
Selecting the target year
by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin
Ri, Chang-Seop ; Yang, Seung Tae ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 221~227
The fiber optic fluorosensor that shows a specific selectivity for calcium ion is studied. This sensor employs protein Calmodulin(CaM) which forms a fluorescent chelate with
. A dialysis membrane is used to entrap a fluorescein isothiocyanate-labeled CaM solution at the common end of a bifurcated fiber optic bundle. The sensing mechanism of this sensor is based on the shifts in the fluorescence spectrum of metal-calmodulin complexes which FCaM forms a chelate with
. Upon binding with
, CaM undergoes a conformational change which induces a change in the fluorescence of FCaM. This change in fluorescence signal which is measured by photomultiflier tube is related to the concentration of
for calibration curve. Detection limit for
and the interference effects by
for this sensor are studied. Response time and life time for this fluorosensor are also investigated.
Determination of free amino acids in the plasma samples of normal subjects and schizophrenic subjects in Korea by HPLC
Park, Seong Soo ; Park, Song-Ja ; Pyo, Hee Soo ; Park, Jongsei ; Park, Taek Kyu ; Shin, Young Min ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 229~236
Multiple-step gradient method was used for the analysis of free amino acids in physiological fluids by high-performance liquid chromatography with diode array detector on the Amino Quant
column under the condition of pH 7.2 of buffer solutions. Plasma samples of normal Korean people and abnormal Korean people who have schizophrenia were subjected to derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid. Quantitative analysis of amino acids in physiological fluids by internal standard method gave highly reproducible results within a relative standard deviation of less than 2~6%. And amino acids amounts of physiological fluids of Korean people gave some different results from those of foreigners. There was large differences in tyrosine amount between normal and abnormal man.
Simultaneous Analysis of Pesticide Priority Pollutants in Soil
Lee, Seung-Seok ; Park, Young-Joo ; Lee, Sueg-Geun ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 237~248
The simultaneous analysis of 16 organic pesticides, which are listed as a part of 129 priority pollutants by EPA(Environmental Protection Agency), was performed by GC-ECD(electron capture detector) and GC/MS-SIM(selected ion monitoring). Two extraction procedures from SW-846, sonication extraction and Soxhlet extraction, were somewhat modified and compared as an extraction and concentration method for the analysis of priority pollutants in soil. Accuracy and precision of the methods were reported from the calculation of mean recovery, mean relative standard deviation, and method detection limit.
The study on the spectroscopic and electrical properties of Azomethine compounds
Baek, Dae-Jin ; Oh, Won-Chun ; Ko, Young-Shin ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 249~258
The polymers, polyazine, polyazomethine ( I ) and polyazomethine ( II ) were prepared by the condensation of p-benzoquinone with hydrazine hydrate, p-phenylenediamine and diaminomaleonitrile in DMSO, respectively. The IR spectra of these polymers showed a characteristic peak of schiff base (-C=N-) at about
. These polymers dissolved in concentrated sulfuric acid showed UV/VIS absorption near 300nm indicatiog the presence of iminium ion(>
). Another UV/VIS absorption band in the region of 350~415nm was shown presumably due to the charge transfer transition in the molecule. The electrical conductivities of polyazine, polyazomethine ( I ) and ( II ) were measured. The self-electrical conductivity of these was found to be about
and these polymers doped with
showed the maximum conductivity of about
Separation of Hg(II) by using the poly-NTOE(1, 12-diaza-3, 4:9, 10-dibenzo-5, 8-dioxacyclopentadeca-1, 12-ylene-2, 7-dihydroxyoctamethylene) in Liquid Chromatography
Kim, Hae-Joong ; Shin, Young-Kook ; Kim, Si-Joong ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 259~264
The separation efficiency of metal ions by using the poly-NTOE(1, 12-diaza-3, 4:9, 10-dibenzo-5, 8-dioxacyclopentadeca-1, 12-ylene-2, 7-dihydroxyoctamethylene) has been determined by column chromatography in aqueous solution. Binding constants and separation factors for several poly-NTOE interactions were measured in aqueous solution. The order of these binding constants and separation factors with metal ions were Co(II)
Zn(II) for the transition metal ions and Cd(II)
Development of Schiff Base Column and Glow Discharge Detector for HPLC : Preliminary Study I
Kang, Mi-Ra ; Kim, Eun-Soo ; Shin, Jung-Sook ; Park, Hyun-Kook ; Yang, Jung-Sung ; Lee, Sang C. ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 265~272
Schiff base ligand columns and glow discharge detector have been developed for the trace analysis of metal ions desolved in water. Various types of hydrazide Schiff base ligands have been used and, additionally, they were examined as a filling material of a HPLC column. The hydrazide Schiff base ligands used were N, N'-oxalybis(salicylaldehydrazone) (OBSH), N, N'-malonylbis(salicylaldehydrazone) (MBSH), and N, N'-succinylbis(salicylaldehydrazone) (SBSH). A mixture of Schiff base ligand and poly(styrene divinylbenzene) was examined and it showed a smooth flow of solution. The OBSH-polymer column demonstrated different effluent factors for different metal ions. Metal ions in eluates were detected by Hollow Cathode Glow Discharge-Atomic Emission Spectrometry(HCGD-AES). HCGD-AES showed good sensitivity and selectivity. This is only the preliminary results of new OBSH-polymer column and glow discharge detector.
Preparation and analysis of lyophilized whole blood as external quality control materials for Pb and Cd determination by graphite furnace atomic absorption spectrometry
Lee, Kong-Joo ; Lim, H.B. ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 273~279
Lyophilized whole blood samples containing various concentrations of Pb and Cd have been prepared as external quality control materials. These materials have been characterized with graphite furnace atomic absorption spectrometry(GFAAS), The optimized conditions for the quantitative determination of Ph and Cd in whole blood using GFAAS were obtained at the ashing temperature of
with 0.1% ammonium dihydrogen phosphate and 0.1% Triton X-100 as matrix modifier. Homogeniety and stability of the prepared whole blood have been studied at the optimized analytical condition.
Effect of Oxidant Concentration and Reflux Time on COD Value by Alkaline Permanganate Method
Czae, Myung-Zoon ; Kim, Mi-Kyung ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 281~284
The effect of permanganate concentration and refluxing time on the test COD results were investigated, as a validation test of the revised procedure. The amended protocol involves the use of a more dilute(1/2.5) permanganate solution(0.010N) and a shorter(1/3) refluxing period(20 min). This revision resulted only 70% of the COD value determined by the old procedure with 8 somewhat improved precision(CV 4.6%
3.3%). The dominant factor that influences the decrease in the COD result is the oxidant concentration, being two thirds of the overall effect. The same results were obtained in the comparison test using seawater samples.
Determination of Uranium using 1-(2-Pyridylazo)-2-naphthol by Adsorptive Stripping Voltammetry
Kim, Kyoung Tae ; Choi, Won Hyung ; Lee, Jin Sik ; Choi, Sung Yung ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 285~292
Uranium has variable oxidation states(
) and 1-(2-Pyridylazo)-2-naphthol forms a very stable chelate with Uranium(
). The determination method of Uranium(
) in 0.1M Borate buffer(pH 7.10) has been investigated by adsorptive stripping voltammetry. The optimum conditions were PAN concentration of
, accumulation potential of 0.00V(vs. Ag/AgCl) and accumulation time of 120sec. The calibration curve was linear over the range of
and the various metal ions did not interfere with the determination Uranium(
) except Cu(II) and Co(II).
Study of the Stability of Brass Coated on Steel Cords with pH and Applied Constant Potential Changes in Aqueous Solutions by AC Impedance Measurements
Ko, Young Chun ; Chung, Keun Ho ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 293~298
The stability of brass coated on steel cords with pH and applied constant potential changes in aqueous solution has been investigated by AC impedance measurements. In solutions of the constant pH, as a applied constant potential is shifted to positive potential, the coating pore resistance is reduced. The fact indicates that as a applied constant potential is shifted to positive potential, the brass coated is dissolved more in solution. The stability of brass coated on steel cords decrease in the order pH=7.1 > pH=4.0 > pH=10.0. The above results are demonstrated by the data of scanning of electronic microscopy(SEM)/energy dispersive spectrometer(EDS).
Separation of Cu(II) from Metal Mixture Solution Using a Hexadecyl
-Octadecylsilanized Silicas(ODS) in Liquid Chromatography
Shin, Young-Kook ; Kim, Si-Joong ; Kim, Hae-Joong ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 299~304
The adsorption behaviors of Ba(II), Cr(II), Fe(II) and Cu(II) on the N, N'-bispalmtoyl 1, 12-diaza-3, 4:9, 10-dibenzo-5, 8-cyclopentadecane (hexadecyl
)-octadecylsilanized silicas(ODS) were investigated with water as the mobile phase. Binding constants for metal ions were measured in aqueous solution. The order of binding constants(K) and the degree of sorption(E) were Ba(II)
-octadecyisiianized silicas(ODS) increased with concentration of metal ions, and the degree of adsorption was found to be affected by the cation-chelation mechanism. The experimental results showed good efficiency for separation of Cu(II) from mixtures of Ba(II), Cr(II), Fe(II) and Cu(II) in aqueous solution.
The Complex Formation of Fe(III)-Salicylic acid
Cha, Ki-Won ; Park, Kwang-Won ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 305~311
The stability constant of the complex between salicylic acid(
) and Fe(III) ion has been determined using UV-Vis absorption spectrometry and pH titration method in O.1M
aqueous solution at
and compared with reference value,
, respectively and the
of 1:1 complex Fe(III)-
Surface-Enhanced Raman Scattering Spectroscopic Identification of Genotoxic Nucleobase Adducts
Kim, Jae-Ho ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 313~319
Surface-enhanced Raman scattering(SERS) spectroscopy was employed to analyze the genotoxic nucleobase adducts of benzo[a]pyrene(BP) formed through one-electron oxidation pathway. SERS spectroscopy provided sufficient resolution to distinguish if BP intermediate was bound to different nucleobases(e. g. adenine or guanine). Furthermore, SERS specroscopy was also able to detect the difference in the binding position of the adduct to the various sites of the nucleobase. The linearity of the calibration curve for N7Ade-BP ranged from 20 picogram to 800 nanogram per microliter and the detection limit under the current conditions was determined 20 picogram per microliter in a solution volume of 20 microliter.
Studies on Partition and Extraction Equilibria of Metal-Dithiocarbamate Complexes(II). Metal Complexes of Ammonium Pyrrolidine Dithiocarbamate
Lee, Jong-Sun ; Choi, Jong-Moon ; Choi, Hee-Seon ; Kim, Young-Sang ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 321~334
Basic studies for the effective extraction of ammonium pyrrolidine dithiocarbamate(APDC) complexes of Co(II), Ni(II) and Cu(II) into a solvent have been performed. The maximum distribution ratio was appeared (log D=1.3543) at pH 2.0 and the partition coefficient was 2.489 in the extraction of
APDC itself into chloroform. From the UV/visible spectra of metal-chelates in aqueous and organic solutions, the pH to form stable 1:2 metal-ligand complexes were Co(II):5.0, Ni(II):8.0 and Cu(II):8.0, respectively and only 1 minute was enough to be partitioned into the chloroform. Besides, the partition and extraction equilibria of the complexes were investigated by back-extracting
metal-chelates from the solvent into an aqueous solution beacuse of their slight solubilities in the aqueous solution. The distribution coefficients and extractabilities were as follows : at pH 6.5~8.5 of the aqueous solution, log D=2.834 : E(%)=99.9% for
, at pH 11, log D=5.699 E%=100 for
, and at pH 6.0, log D=2.025 : E(%)=99.1% for
. And the extraction and formation constants were log
, and log
, respectively. From these results, an optimum extraction procedure can be constructed for the separative concentration of trace metallic ions, and the quantitative determination of them in advanced materials and environmental samples will be expected without any influence of sample matrixes.
Automatic On-line BOD Measurement System Using A Microbial Membrane Electrode
Oh, Hyuk ; Kim, Hai Dong ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 335~340
Automatic biochemical oxygen demand(BOD) measurement system has heen developed using a microbial membrane electrode, prepared from Bacillus subtilis and polyvinyl alcohol(PVA). The automatic BOD measurement system showed a linear response curve up to BOD 60 ppm using a glucose/glutamic acid standard solution, and all the BOD measurement processes are carried out automatically to calculate BOD whithin 10 min after each sample injection. The response times of the microbial electrode was 5 minutes with a 5 min recovery time between measurements and the relative error of the BOD estimation was within 10%.
Analysis of magnetic properties for the melt-spun
Chung, Kang-Sup ; Sung, Hak-Je ; Kim, Kun-Han ; Park, Yun-Chang ; Shu, Su-Jeong ; Lee, Kyeong-Sub ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 341~350
ribbon alloy was manufactured with using melt-spinning method and analyzed the magnetic properties according to the manufacturing conditions. The microstructure and magnetic properties of melt-spun ribbons are sensitively dependent on the quench rate and annealing conditions. As-quenched
ribbons with optimum magnetic properties are obtained at wheel speed(
) of about 20m/sec and over quenched ribbons show optimum magnetic properties at
=22m/see when annealed for 30 minutes at
under vacuum. The crystallization temperature(
phase is about
in the DTA analysis.
A Study of Oxidation Behavior on the Surface of Nd-Fe-B Ribbon Alloy by X-ray Photoelectron Spectroscopy
Chung, Kang-Sup ; Sung, Hak-Je ; Kim, Kun-Han ; Park, Yun-Chang ; Lee, Kyoung-Chul ; Shu, Su-Jeong ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 351~358
Oxidation behavior on the surface of Nd-Fe-B ribbon alloy has been studied by X-ray Photoelectron Spectroscopy. In the incipient stage of oxidation on the surface of "as-received" ribbon Nd-oxide film was formed from the fast oxidation of Nd and Fe was metal state in bulk. In process of oxidation time Fe was more abundant in the outmost surface of ribbon from the defused Fe through Nd-oxide film and layer structure of oxidation film was formed.
Two-enzyme coupled fluorometric assay of urinary dipeptidase
Park, Haeng Soon ; We, Jeoung Soon ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 359~364
Urinary dipeptidase(Udpase) was assayed by fluorometric analysis of NADH which was produced from an indicator enzyme, L-alanine dehydrogenase. The reaction mixture was consisted of a dipeptide(L-ala-L-ala),
, L-alanine dehydrogenase in 12.5 mM sodium carbonate buffer, pH 9.0, and urinary dipeptidase which initiated the reaction. The fluorescence intensity of NADH was measured as a function of time with the excitation wavelength at 340nm and emission at 460nm. Comparison of this fluorometric method with the conventional spectrophotometric method utilizing glycyldehydrophenylalanine(Gdp) as substrate provided the correlation coefficient of 0.996 and increased the sensitivity more than ten times.
Liver tissue sensor for the determination of dopamine
Hur, Moon-Hye ; Kim, Ki-Myo ; Kim, Young-Hak ; Paek, Kwang-Jin ; Choi, Hyun-Young ; Ahn, Moon-Kyu ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 365~370
A tissue biosensor for the measurement of dopamine has been constructed by immobilizing the slice of Sprague-Dawley rat liver on
-sensing electrode. To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead of the Nernst equation. And then compared the results with those obtained from Nernst equation. When we obtained calibration curves from Nernst equation, there were variances on the slope and the linear range. But from Lineweaver-Burk equation, the scale of variance was small. Response time was reduced from 7~12 minutes to 2~3 minutes.
Analysis of organic germanium, Ge-132
Park, Man Ki ; Park, Jeong Hill ; Han, Sang Beom ; Park, Il Ho ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 371~374
An organic germanium compound, Ge-132, was reported to have interferon inducer activity, anti-tumor activity and anti-viral activity. ICP, AA and colorimetry methods were used for the determination of germanium in Ge-132. However these methods have a problem that they only give an information on the total amount of germanium element, and consequently Ge-132 connot be distinguished form toxic inorganic Ge compounds. To overcome this problem, ion chromatography was used to analyze Ge-132. Ge-132 was separated on Ionpac AS4A column with 1.3mM
buffer(pH=9.2) solution as an eluent and detected by the conductivity detector. Correlation coefficient of the calibration curve was 0.999 and the detection limit measured at S/N ratio of 3 was 50pmol. This method was applicable to the analysis of Ge-132 raw material and Ge-132 preparations.
Analysis of Indole-3-acetic acid from bean sprouts by GC/MSD
Lee, Jayoung ; Han, IIkeun ; Lee, Sangyun ; Hawer, Wooderck ; Yeo, Ikhyun ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 375~381
A gas chromatographic analytical method for IAA was developed. IAA was extracted with 80% acetone & methanol. Sample was macerated with 80% acetone & methanol to extract IAA. After filtration, the solvent was removed and residual IAA was esterified with 14%
/MeOH. The IAA esters can be easily separated with intermediate polar column.
The electrochemical desorption of an eicosanethiol monolayer by electrochemical quartz crystal microbalance
Chung, Chinkap ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 383~389
A new approach for the measurement of the surface coverages of monomolecular films fabricated by spontaneous adsorption of thiol compounds on gold is described. It is based on the mass change measurement with EQCM for the reductive electrochemical desorption of thiol in aqueous KOH solution. The results were compared with that of charge calculation during electrochemical desorption. The surface coverage value for eicosanethiol agrees with that obtained by charge calculation of electrochemical reduction as well as that expected from a geometrical model of the compact monolayer.
Determination of Gold by Flame Atomic Absorption Spectrophotometry using a New Chelating Agent
Lee, Jin-Sik ; Uesugi, Katsuya ; Thoru, Nagahiro ; Choi, Won-Hyung ; Kim, Kyung-Tae ; Choi, Sung-Yung ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 391~396
A rapid preconcentration method based on solvent extraction is described for the determination of gold by flame atomic absorption spectrophotometry. Trace amounts of gold was extracted as a new chelating agent, 3-thiophenaldehyde-4-phenyl-3-thiosemicarbazone from pH 4.0 in diisobutyl ketone. The method is simple, fast, free from the effect of many interfering ions and has a high sensitivity and a good precision. Gold is quantitatively separated and concentrated from the elements in standard sample, and the value of the recovery was 91.7 and 108.3% by the proposed method.
Separation of Th(IV) and U(VI) Using Arsenazo I-XAD-16 Chelating Resin
Suh, Jung-Min ; Kim, Min-Kyun ; Lim, Jae-Hee ; Lee, Chang-Hun ; Lee, Won ;
Analytical Science and Technology, volume 8, issue 3, 1995, Pages 397~404
Three Arsenazo I-XAD Chelating resins, with different surface areas and pore sizes were synthesized and characterized. The total sorption capacities of these chelating resins for Cu(II) at pH 5.0 by batch method decreased as follows, Arsenazo I-XAD-16(0.59mmol/g)>Arsenazo I-XAD-4(0.56mmol/g)>Arsenazo I-XAD-2(0.38mmol/g). The sorption and desorption properties of Arsenazo I-XAD-16 chelating resins for U(VI), Th(IV), Hf(IV), Zr(IV), Ni(II), Mn(II), Cd(II). and Cu(II) were studied by both batch and elution method. The Arsenazo I-XAD-16 chelating resin was successfully applied to the separation and concentration of trace U(VI) and Th(IV) in sea and waste waters.