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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Radiation Protection and Research
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Journal DOI :
Korean Association for Radiation Protection
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Volume & Issues
Volume 32, Issue 4 - Dec 2007
Volume 32, Issue 3 - Sep 2007
Volume 32, Issue 2 - Jun 2007
Volume 32, Issue 1 - Mar 2007
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RADIOPROTECTIVE EFFECT OF ALGIN-OLIGOSACCHARIDE THROUGH MEASURING CASPASE-3 AND CASPASE-9 IN MICE
Choi, Seong-Kwan ; Jung, Woon-Kwan ; Lee, Kyu-Soo ; Jang, Young-Il ; Dong, Kyeong-Rae ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 134~139
In order to find out the Radioprotective effect of algin-oligosaccharide(AOS), this study, with a mouse of which whole frame irradiated by 3 Gy radiation once, measured caspase-3 and caspase-9 amid cell signaling connected to apoptosis in order to observe cell activation. In Caspase-3 and Caspase-9 test for observing cell activation, both of Caspase-3 and Caspase-9 showed highly increased O.D. value in the irradiation control group, while the whole groups treated with algin-oligosaccharide before or after irradiation indicated lower O.D. value than the irradiation control group, especially showed big difference in 7 day's treatment group of before irradiation (P<0.001). It confirmed that Caspase generation was restrained in AOS treatment group. Consequently, this study inquired into the fact that algin-oligosaccharide with superior antioxidant activity performed radiation protection by inducing restraint of Caspase generation and confirmed that natural product with less chemical toxicity was able to be applied as radioprotector.
Morphological Changes of Mouse Ovary by X-Ray Irradiation
Yoon, Chul-Ho ; Choi, Jong-Woon ; Yoon, Surk-Hwan ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 140~156
This research was performed to investigate the morphological changes of folliculus ovary according to the radiation dose. The whole body radiation of 200 cGy, 400 cGy, and 600 cGy was given to the each groups of 5 months-aged female mouse. Various staining methods used in this research are: Hematosylin-Eosin method, and immunohistochemistrical methods using BrdU, TUNEL, p53, p21, PCNA and inhibin. The minute structural changes of folliculus ovary were observed through an electron microscope with high magnification. The morphological changes of growing folliculus ovary became distinct as the dose of X-rays increased. Especially, the nuclei of granular cells showed manifest condensation and the changes of the transparent zone were distinct. As a result of histochemical reaction according to Masson's trichrome method and reticular fiber method, the changed granular cells, the deformed basilar membrane of folliculus ovary and the abnormal arrangement of the reticular fiber were observed. In the reaction of BrdU, the granular cells of normal folliculus ovary with positive reaction rapidly decreased according to the increase of the dose of X-rays. In TUNEL study, granular cells showing positive reaction in retarded folliculus ovary were expanded to growing folliculus ovary and primordial folliculus ovary according to the increase of the dose of X-rays. In case of 600 cGy of X-rays, oocyte underwent apoptosis. In p53 immunohistochemistry, p53 manifested to be stronger as the dose of X-rays increased. p53 reactivity was manifested distinctively in all cells comprising folliculus ovary following irradiation of 600 cGy. p21 was manifested in granular cells of folliculus ovary and showed very positive reaction around follicular antrum according to the increase of the dose of X-rays. In PCNA, positive reaction was manifested in growing folliculus ovary, mature folliculus ovary and primordial folliculus ovary, but the extent of the reaction decreased as the dose of the X-rays decreased. The finding that the reaction of granular cells around folliculus ovary was stronger than that near follicular membrane indicates that what was damaged first by X-ray was the cells near folliculus ovary and follicular antrum. The reactivity of
showed difference according to the growing stage of folliculus ovary:
showed the most strong reaction in mature folliculus ovary with follicular antrum. There was strong reaction in granular cells around follicular membrane but
did not occur at all in theca cells comprising follicular membrane.
in ovary tissue exposed to 400 cGy of X-rays was manifested more strongly than in ovary tissue exposed to 600 cGy of X-rays, which was related to the phenomenon that granular cells of mature folliculus ovary underwent necrosis or apoptosis increasingly due to X-rays. In an electron microscope with high magnification, nuclei and protoplasm of granular cells in growing folliculus ovary abruptly underwent minute structural changes according to the increase of dose of X-rays. Cell residue, by-product of cell decease, neutrophil and macrophage around follicular antrum were observed. The minute structural changes in granular cells showed typical characteristics of apoptosis: the increase of electronic density due to nuclear condensation, fragmentation of nuclei and atrophy of protoplasm. Necrosis of cells was identified but it was not so remarkable. Macrophage with apoptotic bodies was scattered. Proportional to the radiation dose, we found that the generation of heterogeneous substance of normal ovary texture's follicular fluid, the emergence of dyeing characteristic in the basilar membrane of folicle, the generation of apoptosis, and the transformation of macrophages, etc. From this results, we can infer the possible radiation hazard on the ovary of cervix cancer patient with radiation therapy.
Fast Neutron Flux Determination by Using Ex-vessel Dosimetry
Yoo, Choon-Sung ; Park, Jong-Ho ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 158~167
It is required that the neutron dosimetry be present to monitor the reactor vessel throughout its plant life. The Ex-vessel Neutron Dosimetry Systems which consist of sensor sets, radiometric monitors, gradient chains, and support hardware have been installed for 3-Loop plants after a complete withdrawal of all six in-vessel surveillance capsules. The systems have been installed in the reactor cavity annulus in order to characterize the neutron energy spectrum over the beltline region of the reactor vessel. The installed dosimetry were withdrawn and evaluated after a irradiation during one cycle and then compared to the cycle specific neutron transport calculations. The reaction rates from the measurement and calculation were compared and the results show good agreements each other.
Neutron Flux Evaluation on the Reactor Pressure Vessel by Using Neural Network
Yoo, Choon-Sung ; Park, Jong-Ho ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 168~177
A neural network model to evaluate the neutron exposure on the reactor pressure vessel inner diameter was developed. By using the three dimensional synthesis method described in Regulatory Guide 1.190, a simple linear equation to calculate the neutron spectrum on the reactor pressure vessel was constructed. This model can be used in a quick estimation of fast neutron flux which is the most important parameter in the assessment of embrittlement of reactor pressure vessel. This model also used in the selection of an optimum core loading pattern without the neutron transport calculation. The maximum relative error of this model was less than 3.4% compared to the transport calculation for the calculations from cycle 1 to cycle 23 of Kori unit 1.
Measurement of Growth Delay and the Oxygen Enhancement Ratio of Fast Neutron Beam Using Mouse Model System
Eom, Keun-Yong ; Park, Hye-Jin ; Kwon, Eun-Kyung ; Ye, Sung-Joon ; Lee, Dong-Han ; Wu, Hong-Gyun ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 178~183
Neutrons are high LET (linear energy transfer) radiation and cause more damage to the target cells than x-rays or gamma rays. The damage from neutrons is generally considered fatal to a cell and neutrons have a greater tendency to cause cell death through direct interaction on DNA. We performed experiments to measure growth delay ratio and oxygen enhancement ratio (OER) in mouse model system. We inoculated EMT-6 cells to the right hind leg of BALB-c mouse and X-rays and neutron beams were given when the average volume of tumors reached
. We irradiated 0, 11, 15.4 Gy of X-ray and 0, 5, 7 Gy of fast neutron beam at normoxic and hypoxic condition. The volume of tumors was measured 3 times per week. In x-ray experiment, growth delay ratio was 1.34 with 11 Gy and 1.33 with 15.4 Gy in normoxic condition compared to in hypoxic condition, respectively. In neutron experiment, growth delay ratio was 0.94 with 5 Gy and 0.98 with 7 Gy, respectively. The OER of neutron beam was 0.97. The neutron beam was more effective than X-ray in the control of hypoxic tumors.
The Effects of Rat's Sperm Bioassay for Low Dose X-Ray
Jin, Gye-Hwan ; Min, Soo-Young ; Lee, Sang-Bock ; Lee, Sam-Yul ; Park, Jong-Bae ; Lee, Jun-Haeng ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 184~189
In order to investigate the enhancement effects of low dose radiation on biological activation, this study applied low dose X-ray to the whole body of male rats to find out whether hormesis is induced in male germ cells. Total 36 Sprague-Dawley(SD) rats as experimental animal were subdivided into 6 groups(in 6 rats per group) such as control, 10 mGy, 20 mGy, 50 mGy, 100 mGy and 200 mGy radiation group All the groups showed slightly increasing number of sperms per 0.1g semen (
respectively), and the motility of sperms amounted to 50.9%, 49.5%, 55.1%, 54.3%, 48.0% and 52.2% respectively. Particularly, compared to the control, the other 5 groups showed higher male hormone level, and the microscopic observations of testicle tissues showed no vacuolization in seminiferous tubules and testis cells. In the results of this experiment, no harmful effect was observed on Sprague-Dawley (SD) rats for which the dose of radiation was controlled as regulated legally by the Ministry of Science and Technology and the Ministry of Health and Welfare. However, as these results were obtained from a limited number of animals, we cannot maintain that the same effect will be observed in the human body. Therefore, there should be further research on the effect on other animals and ultimately on the human body.
SHIELD DESIGN OF CONCRETE WALL BETWEEN DECAY TANK ROOM AND PRIMARY PUMP ROOM IN TRIGA FACILITY
Khan, M J H ; Rahman, M ; Ahmed, F U ; Bhuiyan, S I ; Haque, A ; Zulquarnain, A ;
Journal of Radiation Protection and Research, volume 32, issue 4, 2007, Pages 190~193
The objective of this study is to recommend the radiation protection design parameters from the shielding point of view for concrete wall between the decay tank room and the primary pump room in TRIGA Mark-II Research Reactor Facility. The shield design for this concrete wall has been performed with the help of Point-kernel Shielding Code Micro-Shield 5.05 and this design was also validated based on the measured dose rate values with Radiation Survey Meter (G-M Counter) considering the ICRP-60 (1990) recommendations for occupational dose rate limit (
). The recommended shield design parameters are: (i) thickness of 114.3 cm Ilmenite-Magnetite Concrete (IMC) or 129.54 cm Ordinary Reinforced Concrete (ORC) for concrete wall A (ii) thickness of 66.04 cm Ilmenite-Magnetite Concrete (IMC) or 78.74 cm Ordinary Reinforced Concrete (ORC) for concrete wall B and (iii) door thickness of 3.175 cm Mild Steel (MS) on the entrance of decay tank room. In shielding efficiency analysis, the use of I-M concrete in the design of this concrete wall shows that it reduced the dose rate by a factor of at least 3.52 times approximately compared to ordinary reinforced concrete.