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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Mushroom
Journal Basic Information
Journal DOI :
The Korean Society Of Mushroom Science
Editor in Chief :
Volume & Issues
Volume 13, Issue 4 - Dec 2015
Volume 13, Issue 3 - Sep 2015
Volume 13, Issue 2 - Jun 2015
Volume 13, Issue 1 - Mar 2015
Selecting the target year
Varietal characteristics of cross-bred Cordyceps militaris 'Dowonhongcho'
Lee, Byung-joo ; Lee, Mi-Ae ; Kim, Yong-Gyun ; Lee, Kwang-won ; Choi, Young-sang ; Lee, Byung-eui ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 151~156
DOI : 10.14480/JM.2015.13.3.151
Cordyceps militaris is being studied and cultivated as a medicinal mushroom having many valuable biological and pharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and examined their mycelial growth, mycelial density, and production of stroma and perithecia. Among them selected isolates were crossed and hybrids were produced showing high quality fruiting bodies on artificial media. Mycelial growth rate of new strain 'Dowonhongcho' was higher than that of better on 'Yedang 3' on SDAY at
. The stromata of new strain were club-shaped and bright orange-red. Its height was 6.1 cm and the cordycepin content was 0.34% on average. The new strain showed 9% higher yield than 'Yedang 3' with producing firmer fruit bodies. The optimum temperature for mycelial growth was
and the optimum temperature for stroma development was
. Fruiting bodies were began to produce 45 days later after inoculation. This new cultivar may serve as a valuable one for artificial cultivation and industrial-scale production of C. militaris.
The causes of production decrease in button mushroom of Gyeongbuk province
Kang, Min-Gu ; Jo, Woo-Sik ; Kim, Woo-Hyun ; Lee, Suk-Hee ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 157~162
DOI : 10.14480/JM.2015.13.3.157
This study was carried out to analyze of the cause of button mushroom production decrease of Gyoengbuk province. In 1978, Agaricus bisporus was produced 48,000 ton and exported more than $50 millions. But since 2000, Domestic production of button mushroom was decreased by 70%, and button mushroom farm was also decreased by 37%. Cultivation area was increased by 6%, but Gyeongbuk Province was decreased by 30%. Especially, Production per
was dramatically decreased more than half. There were several causes such as rising labor and material cost, climate changes, and aging of mushroom cultivation farmers. And there was no effort to develop of domestic button mushroom cultivation equipments. One of the main reasons for this reduction was supplied to low quality of button mushroom compost to the farm.
Effects of spent mushroom (Flammulina velutipes) substrates on in vitro ruminal fermentation characteristics and digestibility of whole crop sorghum silage
Moon, Yea Hwang ; Chang, Sun Sik ; Kim, Eun Tae ; Cho, Woong Gi ; Lee, Shin Ja ; Lee, Sung Sil ; Cho, Soo Jeong ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 163~169
DOI : 10.14480/JM.2015.13.3.163
The in vitro experiment was conducted to ensure the supplemental level of spent Flammulina velutipes mushroom substrates (SMS) as an energy source in manufacturing of whole crop sorghum silage. Sorghum harvested at heading stage was ensiled with spent mushroom substrates of 20% (S-20), 40% (S-40) and 60% (S-60) as fresh matter basis for 6 week. The experiment was conducted by 3, 6, 9, 12, 24, 48 hrs of incubation time with 3 replications. The silages were evaluated fermentation characteristics and dry matter digestibility (DMD) in vitro. The pH of in vitro solution was inclined to decrease with elapsing the incubation time, and that of the S-20 was significantly (P<0.05) lower than the other treatment at 48 hr of incubation. Gas production was greater (P<0.05) in the S-20 than the other treatments at 6 and 12 hrs of incubation. The microbial growth in vitro was inclined to decrease following 24 hr of incubation, and thereafter sustained the similar levels. In vitro dry matter digestibility (IVDMD) was lowered by increasing the supplemental level of spent mushroom substrate, and was a low level in the S-60 throughout whole incubation time. Although the IVDMD for S-40 was steadily increased from 9 hr of incubation and reached to similar level with the S-20 at 48 hour of incubation, however SMS for whole crop sorghum silage fermentation might as well add about 20 to 30% in fresh matter basis when considering DMD.
Inhibitory effect of mushrooms extract on TNF-α/INF-γ induced-cytokine in human keratinocytes, HaCaT
Choi, Eun-Ju ; Kim, Eun-Kyung ; Ji, Yong-Seok ; Lee, Chang-Jin ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 170~174
DOI : 10.14480/JM.2015.13.3.170
Mushroom is known for anti-inflammatory and anti-oxidative potential. This study provides evidence that the inhibitory effect of mushroom on the expression of pro-inflammatory cytokines in human keratinocytes, HaCaT cells. To define the underlying mechanisms of action, tumor necrosis factor
-activated human keratinocytes model was used. Mushroom significantly inhibited the expression of cytokines in HaCaT cells. Taken together, the results demonstrate that mushroom inhibited inflammtion, suggesting that mushroom (DW extract: Grifola frondosa Cordyceps militaris), (Ethanol extract: Ganoderma lucidum, Lentinus edodes, Cordyceps militaris, Flammulina velutipes) might be a candidate for the treatment of skin inflammation.
Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates
Min, Kyong-Jin ; Kong, Won-Sik ; Kang, Hee-Wan ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 175~180
DOI : 10.14480/JM.2015.13.3.175
Twenty Inter simple sequence repeat (ISSR) primers were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 Agaricus spp. ISSR primers, (GA)T, (AG)YC, (GA)C and (CTC) amplified PCR polymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted for UPGMA cluster analysis. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with closely genetic relationship of coefficient similarity over 0.92, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese. Furthermore, ISSR-PCR polymorphism could potentially be used to identify homokaryon isolates.
The effect of the mushrooms extract on the PSA expression in prostate cancer cells
Kim, Eun-Kyung ; Tang, Yujiao ; Choi, Heeri ; Choi, Eun-Ju ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 181~184
DOI : 10.14480/JM.2015.13.3.181
Mushroom have long been valued as highly nutritious and tasty foods in many societies throughout the world. It is known for biological activities including anti-inflammatory and anti-oxidative potential. However little is known about anti-cancer property. In this study, we investigated the anti-prostate cancer activity of mushrooms. For that, eight kinds of mushrooms such as, T. matsutake, S. crispa, G. lucidum-US, G. lucidum-AS, C. cardinalis-BR, G. frondosa, P. linteus, U. esculenta were extracted with hot water. Among them, three kinds of mushrooms including T. matsutake, G. lucidum-US and C. militaris-BR extracts inhibited prostate specific antigen (PSA) expression in prostate cancer cell, LNCaP. These results demonstrate that some of mushrooms inhibited PSA expression suggesting that the mushrooms might be a candidate for the treatment of prostate cancer.
Physiological activitive of Grifola frondosa by log cultivation and bottle cultivation
Kim, Jong-Bong ; Seo, Hye-Young ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 185~191
DOI : 10.14480/JM.2015.13.3.185
This research was carried out to determine the differences of physiological activites between Grifola frondosa of log cultivation(LC) and Grifola frondosa of bottle cultivation(BC). Total flavonoids content, total phenolics content, electron donating ability(EDA), nitrite-scavenging ability(NSA), SOD-like activity and inhibitory effect of Xanthine oxidase were examined. The highest value of total flavonoid content is
in water extract from Grifola frondosa of log cultivation at
(LC-W40) but, one of total phenolics compound is
in water extract from Grifola frondosa of bottle cultivation at
(BC-W40). The EDA using DPPH of BC-W40 extract showed the highest value of
. Nitrite-scavenging ability was
in extract from Grifola frondosa of BC-W40 at pH 1.2. The value was SOD-like activity showed the highest value of
in extract from LC-W40. Xanthine oxidase inhibitory activity was the highest value of
in extract from Grifola frondosa BC-W40, and dependent on concentrations. These results showed that a the antioxidant effects of Grifola frondosa is excellent. However, physiological activities of Grifola frondosa were not depend on caltivation method regulary, and were different according to kind of solvents, concentraitions and physialogical factors examined such as EDA, SOD-like activity and NSA.
Physiological activities of water extract and solvent fractions of Grifola frondosa
Kim, Eun-Jung ; Kim, Jun-Ho ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 192~198
DOI : 10.14480/JM.2015.13.3.192
This study was performed in order to analyze the fibrinolytic, thrombin inhibitory, anti-oxidative, acetylcholinesterase inhibitory, and immuno-enhancing activities of the water extract and solvent fractions isolated from Grifola frondosa. Fibrinolytic activity was analyzed using the fibrin plate method, and thrombin inhibitory activity was assayed using the substrate H-D-Phe-piparg- pna. Anti-oxidative activity was estimated using the DPPH assay, and AChE inhibitory activity was measured using the spectrophotometric method. Immuno-enhancing activity was examined using the nitric oxide (NO) production in RAW 264.7 macrophage cells. Cell viability was determined using the MTS assay. Fibrinolytic activities were the highest in water extract (1.55 plasmin units/mL) followed by water fraction (0.85 plasmin units/mL). The thrombin inhibitory activities of the water and ethyl acetate fractions were determined to be 76.43% and 72.59%, respectively. The acetylcholinesterase inhibitory activities of chloroform and hexane fractions exhibited values of 95.14% and 94.74%, respectively. The butanol fraction showed the highest anti-oxidative activity at 94.47%. Anti-proliferating activity against Raw 264.7 cells showed no cytotoxicity. The production of NO in Raw 264.7 cells increased up to 2-fold by adding the water fraction compared to the untreated control. These results suggest that Grifola frondosa may serve as a useful functional food for the enhancement of immune function and the prevention and therapy of cardiovascular diseases.
Analysis on the characteristics of mushroom exports in Gyeongbuk province, Korea
Jo, Woo-Sik ; Park, Ju-Ri ; Kang, Min-Gu ; Kim, Woo-Hyun ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 199~202
DOI : 10.14480/JM.2015.13.3.199
The purpose of this study is to address a current mushroom export situation in Gyeongbuk area for establishing exports strategy, and policy of mushrooms export. Mushroom industry has made a rapid growth over the past 20 years in Korea. Gyeongbuk province exports volume of enoki and king oyster mushrooms account for about half of the country, enoki and total exports in 2009, and 81%, increased to 14% in 2010 and 2010, the maximum exports (9,415 tons) and the maximum exports (14,840 grand)was recorded. But, enoki and total exports in 2011, and -15%, decreased to -19% in 2012, and increased to 32% in 2013, 1% in 2014. King oyster mushroom to represent the Netherlands in Europe, exports were higher exports to China is negligible. In the case of China in 2001, 5 spots automated mushroom factory, production, but only 8,000 tons in 2011, 652 mushroom factory, production of 1,100,000 tons and in 2012, 788 mushroom factory, production of 1,520,000 tons quickly grew.
Characteristics of 'Baekjung', a variety adaptable to high temperature in Flammulina velutipes
Kim, Eun-Sun ; Woo, Sung-I ; Oh, Minji ; Oh, Youn-Lee ; Shin, Pyung-Gyun ; Jang, Kab-Yeul ; Kong, Won-Sik ; Lee, Chang-su ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 203~206
DOI : 10.14480/JM.2015.13.3.203
'Baekjung' adaptable to high temperature was made by crossing between monokaryon derived from selfing of brown strain and monokaryon derived from Korea white strain. In the condition that temperature is maintained at
without low temperature of
suppressing treatment and wrapping during cultivation period, it showed good productivity than Uri1ho(control). The optimum temperature of mycelial growth was
and that of fruiting body initiation and development were
, respectively. The days for the fruiting and yield were 7 days and
g per 1,100 ml bottle, respectively. This variety needed high concentration of carbon dioxide up to 4,000 ppm for the good quality.
The physicochemical properties of kenaf(Hibiscus cannabinus L.) as mushroom culture media source
Kang, Chan-Ho ; Yoo, Young-Jin ; Seo, Sang-Young ; Choi, Kyu-Hwan ; Lee, Ki-Kwon ; Song, Young-Ju ; Kim, Chung-Kon ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 207~211
DOI : 10.14480/JM.2015.13.3.207
To investigate the usefulness of Kenaf(Hibiscus cannabinus L.) as mushroom culture media source, we analyze physical condition and contents of nutritional components. The water absorption rate of Kenaf bast was 578% and it was 95% higher than that of poplar sawdust's. This was caused by Kenaf's porous cellular structure. so it could give more moisture and oxygen to cultured mushroom. Total carbon contents of Kenaf was 91.4%, it was quite higher than that of poplar sawdust, wheat bran and rice bran. Total nitrogen content was 1.76% and C/N ratio was 51.9. The content of NFE(Nitrogen free extract) was 46.6% and it was similar with rice bran. Cellulose content was higher than poplar but lignin content was lower. specially hemicellulose and pectin complex which more digestible carbon source to mushroom was 3.7% higher than poplar. Mineral component and amino acid contents were also maintained high compared with poplar. Fe was 4.2 times, P 3.2 times, K 2.2 times more and Ca was higher 16 mg/kg than poplar. The content of amino acid was quite more higher than poplar sawdust but lower than chaff. Consequently Kenaf had a good trait for basic support material in mushroom culture media and also had a good character as nutritional source.
Characterization of a new commercial strain 'Mongdol' by intra-specific hyphal anastomosis in Pleurotus ostreatus
Oh, Min-Ji ; Kim, Eun-Jung ; Jung, Ji-Hoon ; Shin, Pyung-Gyun ; Kim, Eun-Sun ; Oh, Youn-Lee ; Jang, Kab-Yeul ; Kong, Won-Sik ; Yoo, Young-Bok ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 212~216
DOI : 10.14480/JM.2015.13.3.212
A new commercial strain "Mongdol" of oyster mushroom was developed by hyphal anastomosis. It was improved with hybridization between monokaryotic strain derived from Pleurotus ostreatus ASI 0627 and dikaryotic strain derived from P. ostreatus ASI 2929. The optimum temperature of mycelial growth and fruiting body development were
, respectively. When two different media including PDA (potato dextrose agar medium) and MCM (mushroom complete medium) were compared, the mycelial growth of this mushroom was faster in MCM than in PDA. Similar result was observed with the control strain P. ostreatus ASI 2504. Analysis of the genetic characteristics of the new cultivar "Mongdol" showed a different DNA profile as that of the control strain ASI 2504, when RAPD (Random Amplified Polymorphic DNA) primer URP3 and URP6 were used. Fruiting body production per bottle was about 106 g using demonstration farms. The color of pileus was blackish gray and the stipe was long. Therefore, we expect that this new strain "Mongdol" will satisfy the consumer's demand for high quality mushrooms.
Analysis of phenotypic characterization of segregation population developed by crossing in Flammulina velutipes
Woo, Sung-I ; Kong, Won-Sik ; Kim, Eun-Seou ; Jang, Kab yeul ; Shin, Pyung-Gyun ; Oh, Youn-Lee ; Oh, Min ji ; Nam, Youn-keol ; Kim, Kyung-Soo ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 217~222
DOI : 10.14480/JM.2015.13.3.217
This study was conducted to obtain a growth correlation of basal information from the development of disease resistant Flammulina velutipes cultivars through back-crossing between the strains of wild-type brown monokaryon 4019-20 and the derivative of commercial quality white monokaryons 3. The two strains were selected to back-cross for further enhancing their latent attributes and growth characteristics. The parents of 4019-
back-crossed to reproduce
, M3-Sn. Using
, M3-Sn procured and isolated into 94 monokaryon strains. Further examination of growth characteristics carried out by back-crossing between M3 and
from M3-n dikaryon. Monokaryon exhibited an irregular growth pattern and demonstrated to be sluggish development in the sawdust medium. However
(M3-n) dikaryon strains confirmed mostly regular growth pattern and demonstrated ordinary growth in the sawdust medium. The fruitbody of
confirmed as light-brown colour to be the dominant gene. The colour distributions of fruitbody,
, resulted as follows; 7% of dark brown, 25% of brown, 27% of light brown, 16% of ivory and 25% of white. The ratio of the other color to white showed 3 to 1 which suggested two major genes were related to fruitbody color.
The effect of post-incubation period and temperature treatment on the uniform primordia formation of Pleurotus ostreatus
Jeoung, Yun-Kyeoung ; Baek, Il-Sun ; Kim, Jeong-Han ; Chi, Jeong-Hyun ; Lim, Jae Wook ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 223~227
DOI : 10.14480/JM.2015.13.3.223
The study was conducted to investigate the effects of post-incubation period and temperature treatment conditions during incubation on the uniform primordia formation and cultural characteristics of oyster mushroom (Pleurotus ostreatus). Three kinds post-incubation period; 25, 30, 35 days and control were applied for 30 days while two kinds incubation room temperature
and control were used
. The substrate temperature during pre-incubation was of 'Suhan No. 1' and 'Gonji No. 7'. Oyster mushroom varieties tended to increase between
at 11 to 15 days after inoculation and then they were maintained in treatment temperature during post -incubation period. The
occurrence was at the highest at 6,500 ppm for 'Suhan No. 1' and 5,800 ppm for 'Gonji No. 7' at the time of the highest temperature increase. The ratio of un-uniformal primordia formation and the ratio of non-commercial fruit body were reduced by 40%, 10.5%, respectively compared to control for 'Suhan No. 1' when in the post-incubation temperature was
, and incubated for 10 days and 15 days treatment. Also, 'Gonji No. 7' was reduced by 19%, 9.5%, respectively when in the post-incubation temperature was
, and incubated for 10 days treatment. Therefore, the higher post-incubation temperature of room and longer post-incubation period resulted in the higher percentage of primordia formation of two cultivars.
Characteristic of a new variety Lentinula edodes, 'Nongjin-go'
Moon, Ji-Won ; Lee, Chan-Jung ; Cheong, Jong-chun ; Kong, Won-Sik ; Kim, Kil-ja ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 228~232
DOI : 10.14480/JM.2015.13.3.228
'Nongjin-go' is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryon Lentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 and verified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growth is
and it of fruit-body primodium formation is range from 15 to
. Nongjigo is agricultured at mid-high temperature well. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productions per period is regular than 'Sanjo701'. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As its browning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properly for its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.
Adjusting moisture contents of the substrates on the mushroom bottle cultivation by the device Load cell
Cheong, Jong-Chun ; Lee, Chan-Jung ; Moon, Ji-Won ; Kweon, Jae-Gun ; Kim, Hyuck-joo ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 233~236
DOI : 10.14480/JM.2015.13.3.233
This report is the result of devising a method for utilizing the device of the load cell to maintain a constant water content of the medium every day to prepare a cultural substrates with the mixer for growing mushrooms bottle cultivation. A load cell was device under the medium mixer. It is developed when the device reaches the weight calculated as amount of substrate bottled and number of the bottle, it is automatically terminated by water injection. In addition, measuring the water content of each medium and the total weight of the medium reaches the target moisture content were calculated by using the program Cheong et al. (2015). Enter the total weight of the medium on the display unit of the load cell, when starting the water supply to reach the weight-based mixing media, the water supply is stopped. This method can improve the convenience by reducing the user's trouble in repeated work medium prepared by automating water supply. The suitable moisture content of the mixed medium for some kind of mushroom can be improved by the composition accuracy. And mycelial culture period, primordial period, mushroom growing period is maintained even of the medium can be produced stably. Therefore, it is possible to achieve a stable management of the mushroom farm according to mushroom quality and quantity stable throughout the year.
Development of a new brown button mushroom cultivar 'Hogam'
Oh, Youn-Lee ; Jang, Kab-Yeul ; Kong, Won-Sik ; Shin, Pyung-Gyun ; Kim, Eun-Seon ; Oh, Min ji ; Choi, In-Geol ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 237~242
DOI : 10.14480/JM.2015.13.3.237
A new brown button mushroom cultivar, 'Hogam', C34 line, was made by crossing homokaryons, ASI1164-37 and ASI1175-66, selected by RAPD analysis and by cultivating three times. Mycelium of 'Hogam' on CDA (compost dextrose agar) media grew well at
. The optimum pin-heading temperature of new variety and optimum growing temperature was
. The thickness of the mature cap and stipe were thicker than a control, 'Dahyang' that developed in 2010. The color of pileus was light brown and lighter than 'Dahyang'. Days required from casing to first harvesting were three days longer than control strain, but the weight of harvested fruiting body increased by 1.35 times. 'Hogam' cultivar are expected to contribute to the diversification of domestic mushroom cultivars.
Selection of Agaricus bitorquis hybrid strains based on RAPD analysis
Oh, Youn-Lee ; Jang, Kab-Yeul ; Kong, Won-Sik ; Shin, Pyung-Gyun ; Kim, Eun-Sun ; Oh, Min ji ; Choi, In-Geol ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 243~249
DOI : 10.14480/JM.2015.13.3.243
Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature(
) unlike A. bisporus is cultivate at
. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at high temperature (
). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we made heterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(single spores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated on CDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different media condition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI 1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon that cross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.
Evaluate spent mushroom substrate for raising bed soil of rice
Oh, Tae-Seok ; Park, Youn Jin ; Kim, Tae-Kwon ; Kim, Chang-Ho ; Cho, Yong-Koo ; Kim, Seong-Min ; Shin, Dong-Il ; Koo, Han-Mo ; Jang, Myoung-Jun ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 250~255
DOI : 10.14480/JM.2015.13.3.250
In this study spent mushroom substrate has ingredient raising rice bed soil. spent mushroom substrates are organic content is 60.72% were nitrogen - phosphoric acid - potassium is 1.39 - 0.89 - 0.81% of the chemical characteristics determine. Post-harvested mushroom substrates of the stabilization process, the temperature of the 20 days time progress in the pH of the rise and fall of temperature down were germination index also 77, as identified, Spent mushroom substrate bed soil for raising rice Ingredient to take advantage of the 20 days or more stabilization process needed to be investigated. Rice seed germination characteristic is in the common bed soil for raising rice ingredients manufactured control group and the comparison in spent mushroom substrate is 10% or less of a mixed experimental population of the germination rate is 82% was more than average days to germination and germination energy, even a statistical significant difference is or control group than good level was ok. Growth initial also spent mushroom substrate is 10% or less of a mixed experimental population of shoot dry matter (top) and grave less than control group higher as confirmed spent mushroom substrates are bed soil for raising rice ingredients are likely to take advantage of the high, as was the judge.
The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture
Baek, Il-Sun ; Chi, Jeong-Hyun ; Jeoung, Yun-Kyeoung ; Kim, Jeong-Han ; Lim, Jae-Wook ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 256~261
DOI : 10.14480/JM.2015.13.3.256
This study was conducted to reduce contamination ratio of oyster mushroom bottle cultivation. The optimal conditions of substrate sterilization for reducing of contamination ratio were at
for 90 min. In addition, UV-C irradiation is good for lower contamination ratio to continue over 6 hours at cooling and inoculation room after sterilization. The contamination ratio and density of microorganisms of substrate were showed 0% after sterilization at
for 90 min. Trichoderma sp., main pathogen of mushrooms, was detected from substrate after sterilized during 2 or 4 hours at
, respectively. The amount of electricity used was the lowest at
for 90 min than that of other sterilization conditions. The UV-C irradiation treatment was used UV-C lamp(40 watts) in the inoculation room(
). The density of bacteria did not detected after UV-C irradiation for 6 hours. And the death ratio of bacteria and Trichoderma sp. was 99.9% after UV-C irradiation for 6 hours. However, in the same UV-C irradiation time, the death ration of Cladosporium sp. was 90.9%. Therefore, the death ratio of fungi was lower than that of bacteria at the same UV-C irradiation treatment.
Growth characteristics and productivity of oyster mushroom according to adding of Salvia miltiorrhiza
Lee, Chan-Jung ; Moon, Ji-Won ; Yoo, Young-Mi ; Han, Ju-Yeon ; Cheong, Jong-Chun ; Kong, Won-Sik ; Kim, Young Guk ; Yoo, Young-Jin ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 262~265
DOI : 10.14480/JM.2015.13.3.262
This study was carried out to investigated optimum mixing ratio of Korean cultivation Salvia miltiorrhiza for production of functional oyster mushroom. Mycerial growth was slow at addition of Salvia miltiorrhiza, and significant difference by increase of Salvia miltiorrhiza. Yields of fruiting body show the highest to 139.5 g/850 ml of medium which are addition 5 g/bottle of Salvia miltiorrhiza but rapid decrease by increase of Salvia miltiorrhiza. Diameter of pileus and thick of stipes were higher at addition Salvia miltiorrhiza than those of the controls. Thick of pileus and length of stipes were the highest at addition 30g, and 20 g and 30 g of Salvia miltiorrhiza. The L value of stipes were the highest at addition 20 g and 50 g of Salvia miltiorrhiza and the L value of pileus were the highest at addition 30 g of Salvia miltiorrhiza, but there was no significant difference in the a-value and the b-value.
Optimal additive ratio of barley flour for substitution of rice bran at cultivation of winter mushrooms
Lee, Chan-Jung ; Moon, Ji-Won ; Yoo, Young-Mi ; Han, Ju-Yeon ; Cheong, Jong-Chun ; Kong, Won-Sik ; Lee, Byung-eui ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 266~269
DOI : 10.14480/JM.2015.13.3.266
This study was carried out to investigated optimum additive ratio of barley flour for substitution of rice bran at cultivation of winter mushrooms. Mycerial growth was faster according to increase of barley flour ratio than those of controls, but only some slow at the addition of 10% barley flour. Yields of fruiting body show the highest to 165.4 g/850 ml of medium which are the addition 30% of barley flour and not significant difference of yields up to the addition of 70% barley flour. Diameter of pileus was the highest at the addition of 30% barley flour. Hardness of pileus and stipes were the highest at the addition of 10% barley flour. The L value of stipes were the hight at the addition of barley flour, but the L value of pileus were decreased at the addition of barley flour, but there was no significant difference in the a-value and the b-value.
Selection of parental monokaryons from Korean Hypsizigus marmoreus by protoplast regeneration
Oh, Youn-Lee ; Kong, Won-Sik ; Jang, Kab-Yeul ; Shin, Pyung-Gyun ; Kim, Eun-Sun ; Oh, Min ji ; Choi, In-Geol ;
Journal of Mushroom, volume 13, issue 3, 2015, Pages 270~273
DOI : 10.14480/JM.2015.13.3.270
Hypsizigus marmoreus is commercially the most important edible mushroom in Japan. This mushroom is usually cultivated for a longer period (about 85~120 days) than other mushroom. In order to develop a new cultivar that has a shortened cultivation period, the genome analysis of this strain has been considered. This study aims to obtain parental monokaryotic strains reproducing 'Haemi' cultivar in Hypsizigus marmoreus for reference genome sequencing. The mycelia were cultured in MCM and MYG media for various incubation periods. Homogenized mycelia were treated with commercial cell wall degrading enzymes to maximize protoplasts production yield from Hypsizigus marmoreus. The greatest number of protoplasts was obtained from mycelia cultured in MCM media for 3 days using Novozyme enzyme. The isolated protoplasts were grown in regeneration agar media after two weeks. Regenerated colonies were picked and moved on separated dishes for microscopic observation. Neohaplonts regenerated from dikayotic strains were identified by the absence of clamp connections. We confirmed that one of monokaryotic strains is a parental strain by crossing with an original compatible strain of 'Haemi' cultivar. This parental strain will be used for reference genome sequence analysis.