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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Agricultural Science
Journal Basic Information
Journal DOI :
CNU Journal of Agricultural Science
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Volume & Issues
Volume 39, Issue 4 - Dec 2012
Volume 39, Issue 3 - Sep 2012
Volume 39, Issue 2 - Jun 2012
Volume 39, Issue 1 - Mar 2012
Selecting the target year
Effects of irradiation periods on the Lettuce Growth
Kim, Dong-Eok ; Kim, Bong-Soo ; Kim, Hyun-Bae ; Yoon, Yong-Cheol ; Kim, Chi-Ho ; Kim, Hyeon-Tae ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 459~466
DOI : 10.7744/cnujas.2012.39.4.459
The objective of this study is to observe growth pattern of grand rapids(lettuce) according to different dexperimental conditions for minimizing its growth period such as composition of nutrient, irradiation time of light source and cultivation mode. The culture of water spray type using seeds of grand rapids was performed during growth period of total 50 days. Experimental conditions consist of five compositions of nutrient (PW 0.5 mS/cm, PW 1.0 mS/cm, PY 0.5 mS/cm, PY 1.0 mS/cm, PW 2.0 mS/cm) and three irradiation times of light source (12h(on) / 12h(off), 18h(on) / 06h(off), 24h(on) / 00h(off)). Illumination was adjusted to ratio of 7:1:1 of red, blue and white color, respectively. Indoor environmental condition for cultivating grand rapids is as follows: temperature (19-
), relative humidity (60-70%) and carbon dioxide (1,000-1,200 ppm). The data were obtained from five iteration tests. The maximum growth level was observed in the experimental condition of 18 hr(on) / 06 hr(off) and PY 1 mS/cm for 1st week of cultivation period, 24 hr(on) / 00 hr(off) and PY 1mS/cm for 2nd week of cultivation period, and 24 hr(on) / 00hr(off) and PW 1 mS/cm for 3rd week of cultivation period, respectively. On the contrary, the minimum growth level was observed in the experimental condition of 18 hr(on) / 06 hr(off) and PW 0.5 mS/cm for 1st week of cultivation period, 12 hr(on) / 12 hr(off) and PW 0.5 mS/cm for 2nd week of cultivation period, and 12 hr(on) / 12 hr(off) and PY 0.5 mS/cm for 3rd week of cultivation period, respectively. Based on the results obtained from this study, it is concluded that the growth level of grand rapids varied with different irradiation time of light source and composition ratio of nutrient according to cultivation period.
The protective effect of Perilla frutescens from ONOO
-induced oxidative stress and antiaging effect under cellular system
Kim, Hyun Young ; Hwang, Bo Ra ; Wu, Ting Ting ; Cho, Eun Ju ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 467~471
DOI : 10.7744/cnujas.2012.39.4.467
In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-
porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide (
)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and
. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite (
) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against
itself and its precursors, NO and
. Furthermore, the aging model of hydrogen peroxide (
)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens.
-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.
Application of remote-controlled aerial application to control weeds on the paddy field using imazosulfuron·mefenacet
Won, Ok Jae ; Kim, Bong Hyun ; Park, Kyung Mi ; Park, Su Hyuk ; Pyon, Jong Yeong ; Park, Kee Woong ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 473~476
DOI : 10.7744/cnujas.2012.39.4.473
The experiment was conducted to investigate the herbicidal efficacy of imazosulfuron mefenacet by using remote-controlled aerial application (RCAA) to control annual and perennial weeds in rice paddy field. Eight annual weed species including Echinochloa crus-galli L. and three perennial weeds were dominated in the experimental field. Application of imazosulfuron mefenacet using RCAA was highly effective to control both annual and perennial weed species. No phytotoxic effect was observed in the rice based on the plant height and the number of tillers. Finally, rice yield in the imazosulfuron mefenacet treatment was as much as that in the hand weeding. This study indicates that imazosulfuron mefenacet using RCAA can be applied to control both annual and perennial weed species in rice paddy field.
Mapping quantitative trait loci controlling low-temperature germinability in rice
Nguyen, Hoang Nam ; Park, In-Kyu ; Yeo, Sang-Min ; Yun, Yeo-Tae ; Ahn, Sang-Nag ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 477~482
DOI : 10.7744/cnujas.2012.39.4.477
Low-temperature germination is one of the major determinants for stable stand establishment in the rice direct seeding method in temperate regions and at high altitude areas. Quantitative trait loci (QTL) controlling low-temperature germinability in rice were identified using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon and the Korean japonica cultivar, 'Hwaseongbyeo'. The germination rate at
was measured to represent low-temperature germination and used for QTL analysis. The germination rate at
for 7 days of Oryza rufipogon and Hwaseongbyeo was 93.3 and 28.7%, respectively, and that of progenies ranged from 0 to 48%. A linkage map was constructed using 135 simple sequence repeat (SSR) markers. Five putative QTLs associated with low-temperature germination were detected on chromosomes 1, 3, 4, 10 and 11. The QTL, qltg10 on chromosome 10 accounted for 19.2% of the total phenotypic variation for low-temperature germinability. Four additional QTL, accounted for 10.4 - 15.1% of the total phenotypic variation. The O. rufipogon alleles in all detected QTLs loci increased the low-temperature germination rate. No QTL associated with low temperature germinability has been detected near the qltg10 QTL in this study suggesting that qltg10 is a new QTL. The locus, qltg10 is of particular interest because of its independence from undesirable height and maturity effects. The DNA markers linked to the QTL for low temperature germinability would be useful in selecting lines with enhanced low temperature germinability in rice breeding program.
Preference evaluation of stink bugs to leguminous seeds by video tracking system
Bae, Soon-Do ; Kim, Hyun-Ju ; Yoon, Young-Nam ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 483~489
DOI : 10.7744/cnujas.2012.39.4.483
Preference of stink bugs to various leguminous seeds was evaluated by using VTS (Video Tracking System) in laboratory. Major soybean stink bugs such as bean bug, Riptortus pedestris (Fabricius), one-banded stink bug, Piezodorus hybneri (Gmelin), eastern green stink bug, Nezara antennata (Scott), and sole bug, Dolycoris baccarum (L.) were significantly most attracted to Cheongjakong, a soybean variety, baited fish-net trap, followed by soybean varieties Ilpumgeomjeongkong and Taekwangkong, a peanut variety Daekwangdangkong, a kidney bean variety Gangnangkong, and a adzuki bean variety Jungwonpat, respectively in a soybean field. VTS observation in laboratory showed that R. pedestris and D. baccarum had significantly higher frequency of visit on Cheongjakong, followed by Ilpumgeomjeongkong. However, P. hybneri, N. antennata and Halyomorpha halys (Stal) had significantly higher number of visits on Cheongjakong, Seonnogkong and Jinpumkong, followed by Ilpumgeomjeongkong. The sojourned time of stink bugs, however, was significantly longer on Cheongjakong regardless of species of the bugs. Accordingly, Cheongjakong was evaluated as the most preferred soybean seed by fish-net trap and VTS. Thus, VTS is found to be an effective means to evaluate the food preference of stink bugs.
Survey on current status of vegetable seed markets of Guangdong and Yunnan provinces in China for the development of domestic vegetable seed industry
Kwak, Jung-Ho ; Yoon, Moo Kyoung ; Park, Suhyung ; Kim, Dae-Young ; Cheong, Seung-Ryong ; Shin, Hyun-Ho ; Lee, Sang-Kil ; Lim, Yong Pyo ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 491~496
DOI : 10.7744/cnujas.2012.39.4.491
Vegetable seeds are one of the most important key factors to determine the way of vegetable's production, distribution, processing and storage. Unfortunately, because of international exchange crisis in 1997, the most of main Korean seed companies were merged into foreign capitals. Currently, many domestic seed companies are incapable of their own survey and development of vegetable seed markets in foreign countries. To provide valuable seed market information for these companies, China, especially Yunnan and Guangdong provinces in China, was selected. Since China is one of the major vegetable seed importers. Also, Yunnan and Guangdong provinces are the most promising targets to export Korean vegetable seeds. The current status of vegetable production in China was analyzed with consideration of Yunnan and Guangdong provinces. The contents of survey and analysis are covering major vegetable crops' cultivation area, farm number, cultivation type, production amount, market price, farm income, packaging method, distribution type and amount of seed consumption. And particularly, major horticultural characteristics of leading varieties were presented to assist the development of exportable varieties of domestic seed companies. We assume the results of this study would be practically usable for the development of exportable varieties.
Development of new broccoli varieties from elite lines obtained by microspore cultivation method
Kwak, Jung-Ho ; Park, Miyoung ; Lee, Jun-Gu ; Park, Suhyung ; Kim, Dae-Young ; Jeong, Seung-Ryong ; Lim, Yong Pyo ; Yoon, Moo Kyoung ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 497~502
DOI : 10.7744/cnujas.2012.39.4.497
Since the year 2000, the production and consumption of broccoli have rapidly increased in Korea. And, the average production area and amount were about 1,700 ha and 29,000 ton for the past 5 years. Even with the increase of these cultivation and consumption, more than 95% of the broccoli seeds are currently imported from foreign countries such as Japan and Netherlands. Therefore, development of domestic broccoli varieties is needed to relieve Korean farmers' production cost for broccoli. In this situation, National Institute of Horticultural and Herbal Science (NIHHS) of Korea has tried to develop F1 hybrid varieties from elite lines that were obtained by microspore cultivation method from 2008. As the results, about 850 lines of broccoli were obtained and self-pollinated. Then their ploidy levels of the genome were confirmed to select double haploid (D.H.) lines. And the D.H. lines' horticultural traits were evaluated in open field. After the selection of 17 elite D.H. lines, they were cross-pollinated with a male sterile (MS) line to produce F1 hybrid seeds. After 2 to 3 years field trials of these F1 hybrid varieties at the area of Suwon, Gangneung, and Jeju respectively, two hybrid varieties such as 'Wongyo8011' and 'Wongyo8012' are selected for the application of variety protection. With these 4 years of research, we found that the microspore cultivation method is a powerful tool for the conventional breeding program, especially for the development of various inbred lines and even F1 hybrid varieties in short time.
Effect of preharvest aminoethoxyvinylglycine spraying time on fruit quality of 'Nagasawa Hakuho' peach (Prunus persica Batsch) during shelf-life
Wang, Mao-Hua ; Lee, Ug-Yong ; Oh, Kwang-Suk ; Lee, Eun-Gu ; Ahn, Young-Jik ; Hwang, Yong-Soo ; Chun, Jong-Pil ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 503~509
DOI : 10.7744/cnujas.2012.39.4.503
This research aimed to investigate the effect of preharvest spray of aminoethoxyvinylglycine (AVG) on fruit quality of 'Nagasawa Hakuho' peach (Prunus persica Batsch). The efficacy of various concentrations of AVG (75 and 150 mg/L), which inhibits the ethylene biosynthesis, was evaluated under field conditions. Treatments were performed 21, 14 and 7 days before harvest, respectively. Fruit weight, flesh firmness, soluble solids content, acidity, ethylene production, respiration rate and skin color difference were determined at harvest time and during shelf-life at
. Results indicated that the most appropriate timing of AVG spraying was 21 days before harvest at the concentration of 75 mg/L as shown higher firmness at harvest time and lower ethylene production and respiration rate during shelf-life. Spraying of AVG during near harvest period (14 to 7 days before harvest) inhibited fruit growth slightly, independent of concentration and did not affect fruit quality in 'Nagasawa Hakuho' peach.
Antioxidant activity of black rice and grains
Cho, Eun Ju ; Choi, Mi Jin ; Shin, Seon Hwa ; Kim, Hyun Young ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 511~514
DOI : 10.7744/cnujas.2012.39.4.511
In this study, the comparison of antioxidative effect of the methanol (MeOH) extracts from grains were investigated in vitro radical scavenging system. Ten grains (black rice, rice, barley, wheat, millet, sorghum, glutinous millet, buckwheat, phellines linteus rice and brown rice) were extracted with MeOH. Among the MeOH extracts of grains, sorghum and black rice showed effective scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The
values of sorghum and black rice were
, respectively. In addition, black rice also exerted the strongest activities on hydroxyl radical (
) scavenging activity. Furthermore, the MeOH extracts of black rice showed effective and dose dependant scavenging activities of DPPH radical and
. These results indicated that black rice showed strong free radical scavenging activity. It suggested that black rice could be a promising natural antioxidant against free radical-induced oxidative damage.
Preliminary screening of leafy vegetable New Zealand spinaches (Tetragonia tetragonioides) native to Korea
Kim, In-Kyung ; Lee, Ka Yeon ; Kim, Sung-Ki ; Kim, Byung-Woon ; Choi, Weon-Young ; Lee, Geung-Joo ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 515~523
DOI : 10.7744/cnujas.2012.39.4.515
Recent development and enlargement of reclaimed lands along the sea shores required to explore alternatives to existing crops, which are suitable to grow well and to declare higher profits. The objectives of this study were to investigate yield-related parameters for development of new leafy vegetables, and to screen some candidates among New Zealand spinach genotypes which were collected in Korea. Initially all the collected genotypes were grown for 7 weeks in a greenhouse of the experiment field in Chungnam National University, and then transplanted into the field to measure morphological or physiological parameters (plant height, branch number, stem diameter, chlorophyll content, and maximum canopy diameter), and yield-related parameters (leaf number, leaf length, leaf width, fresh weight and dry weight). Those parameters were quantitatively measured at 1, 5 or 9 weeks after transplanting (WAT). Parameters exhibiting statistically significant difference among the accessions were plant height, branch number, maximum canopy diameter, chlorophyll content, and leaf length. In contrast to highly correlated parameters with fresh and dry weight obtained in salt-affected soil in the earlier report, fresh weight was correlated significantly with leaf length (r=0.72), leaf width (r=0.64), and canopy diameter(r=0.66), while dry weight was correlated significantly with plant height (r=0.46), leaf length (r=0.72), leaf width (r=0.73), fresh weight (r=0.79), chlorophyll content (r=0.47), and canopy diameter (r=0.87). Based on the significantly correlated with yield parameters, the candidate accessions which were ranked in top statistical groups include CNU06A01, CNU06A13, CNU06A26, CNU06A35, CNU06A38, and CNU06A55. In order to be cultivated in reclaimed lands, it is necessary to screen out salt tolerant accessions among the above high-yielding genotypes.
Vegetation structure and ecological properties of Picea jezoensis community
Do, Mi-Sole ; Lee, Ji-Hye ; Gwon, Jae-Hawn ; Song, Ho-Kyung ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 525~534
DOI : 10.7744/cnujas.2012.39.4.525
This study was conducted to classify community and verify vegetation structure of Picea jezoensis community in subalpine zone of Mt. Kyebang, Mt. Jiri, Mt. Deogyu, 39 releves set up and was carried out to analyze ordination. P. jezoensis Community was classified into Acer ukurunduense subcommunity, Quercus mongolica subcommunity, Syringa reticulata var. mandshurica subcommunity and P. jezoensis typical subcommunity. The order of important value of the forest community with DBH 2cm above plants was P. jezoensis (89.22), Acer komarovii (30.50), Abies koreana (18.81), Rhododendron schlippenbachii (18.10), Sorbus commixta (17.19), Betula ermanii (16.89), Pinus koraiensis (16.80), Taxus cuspidata (12.45), Acer ukurunduense (11.73). The DBH analysis suggests that P. jezoensis will maintain dominant position.
A plan for the development of botanic garden displays using local landscape resources
Park, Eun-Yeong ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 535~543
DOI : 10.7744/cnujas.2012.39.4.535
Botanic gardens are steadily increasing based on people's increased interests in environment and ecology, lengthened leisure hours and improved transportation. However, similar florae and undifferentiated display are considered as problems, while their functions, purposes and characteristics have been more diversified. This study aims to investigate the present conditions and problems of display at botanic gardens and to find out solutions to make them exhibit plants through various ways of display and have their own characteristic, through a case study of seven botanic gardens. As botanic gardens are being recognized as a cultural institution, they should have limitations in the aspect of places that simply collect and exhibit rare plants. The current problems are unclear setting of design goals and communication with visitors. The gardens should escape from the existing supplier-oriented view to a visitor-oriented view, thinking about what the visitors will be able to see and get there. In particular, their display lacks differency, aesthetics, eye-level display, and multi-layered display. In addition to the essential functions of collecting the world's plants, exhibiting them according to purposes and giving scientific learning, botanic gardens should also show a sense of seasons with plants, trigger interests and amusement through unique plants, make visitors more interested in florae and closer to plants, and include social functions. Botanic gardens should be capable of leaning resources display, speciated display, complex and convergent garden-type display, and display fit for local and cultural contexts.
Insect natural enemies as bioindicators in rice paddies
Ueno, Takatoshi ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 545~553
DOI : 10.7744/cnujas.2012.39.4.545
In Asia, including Japan and Korea, rice paddies occupy the largest cultivated area in agricultural land. Rice paddies provide the habitats for many organisms including endemic species, sustaining high biodiversity. Insect natural enemies inhabiting rice paddies have an important function for rice production as agents of 'ecosystem services' because they play a major role in suppressing rice pests. The diversity and abundance of natural enemies can be a good index reflecting the 'healthiness' of agro-ecosystem services in rice paddies. The present study investigates whether insect natural enemies could be good biological indicators for general arthropod biodiversity and agricultural practice. First, the concept of ideal bio-indicators was summarized. The strategy to explore and select such bio-indicators was then proposed. Lastly, field survey was made to evaluate the abundance and biodiversity of natural enemies in Japanese rice paddies where chemical inputs, i.e., insecticide use, were different. The results showed that reduction of chemical inputs led to an increase in species richness or diversity of natural enemies including parasitoids and predators. Then, the data were analyzed to examine suitable indicator species to assess environmental soundness of agricultural practice and biodiversity in rice paddies. The density of several species of natural enemies did respond both to pesticide use and to general arthropod biodiversity. The analyses thus have indicated that natural enemies can be suitable as bio-indicators. Usefulness of indicator species in rice paddies is discussed in the context of ecologically sound agriculture.
Effects of supplementing distillers dried grains with solublesin the diet of lactating sows onvariation in the body weight of piglets
Song, Minho ; Kim, Do-Myung ; Choi, Kyu-Myung ; Seo, Seongwon ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 555~559
DOI : 10.7744/cnujas.2012.39.4.555
Distillers dried grains with solubles (DDGS) is a by-product from processing corn to produce bio-ethanol, and it contains almost three times higher protein, fat, and fiber than corn. Intake of higher amount of nutrients originated from corn in lactating sows may contribute to changes in milk composition and thus alter the growth of suckling piglets. Therefore, the objective of this study was to evaluate effect of supplementing DDGS in the diet of lactating sows on variation in the body weight of piglets. A total of 50 sows with parities of 3 to 5 were usedand allottedinto one of four treatments which included 0 (CON), 10% (DDGS10), 20% (DDGS20), or 30% (DDGS30) DDGS in the diet. Diets were fed to sows during the whole lactation period. Individual body weights of piglets were measured within 24 h after farrowing and at weaning. No significant differences were observed inthe coefficient of variation (CV) for body weight of piglets within a litter after farrowing and at weaning among the treatments (p>0.05). There was, however, a tendency of decreases in the CV for body weight of piglets within a litter at weaning compared with that after farrowingin the DDGS10 and DDGS20 treatments. At weaning, the percentage of the number of piglets weighing less than 4.5 kg in DDGS20 (4.86%, 7 out of 144; p<0.05) or DDGS30 (5.04%, 6 out of 119; p=0.059) was lower than that in CON (12.41%; 17 out of 137) although this pattern was not observed in DDGS10. In conclusion, addition of DDGS in lactating sow diets improved piglet performance by reducing the number piglets weighing less than 4.5 kg.
Isolation of whey protein and hydrolysis pattern of whey protein by proteolytic enzyme
Renchinkhand, Renchinkhand ; Bae, Hyoung Churl ; Jeong, Seok Geun ; Nam, Myoung Soo ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 561~568
DOI : 10.7744/cnujas.2012.39.4.561
The aim of this study was to introduce a simple method for isolation of
-lactoglobulin and bovine serum albumin from cow's milk, and peptides produced by enzymatic hydrolysis of
-lactoglobulin and bovine serum albumin with alcalase. Whey protein were precipitated from whey by ammonium sulfate and,
-lactoglobulin were isolated using Hi Prep 26/60 Sephacryl S-100 column gel filtration chromatography. Bovine serum albumin and
-lactoglobulin were isolated by Mono-Q 5/50 GL column anion exchange chromatography of the 50% Ammonium Sulfate-supernatant. Isolated whey proteins were hydrolyzed by proteolytic alcalase. Tricine SDS-PAGE and reverse-phase HPLC analyses revealed that almost hydrolyzed all the
-lactoglobulin and bovine serum albumin with alcalase. Molecular weight of various peptides derived from alcalase hydrolysate were small molecular weight than 3.5 kDa.
Effect of organic acid and water washing on meat quality and surface population of E. coli of pork loin
Seol, Kuk-Hwan ; Kim, Hyoun Wook ; Jang, Oun-Ki ; Oh, Mi-Hwa ; Park, Beom-Young ; Ham, Jun-Sang ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 569~575
DOI : 10.7744/cnujas.2012.39.4.569
This study was performed to investigate the effect of a sequencial washing of organic acid and distilled water on meat quality and the population of Escherichia coli of pork loin during cold storage. E. coli ATCC25922 was inoculated on the surface of sliced pork loin and 50 mL of 1% or 2% of organic acid and the same amount of distilled water was sprayed on the surface of pork loin. Then, physicochemical and microbial properties of pork loin were analyzed during cold storage. During storage period, lightness, yellowness and lipid oxidation (thiobarbituric acid reactive substances value) was higher than those of not treated control. However, the population of E. coli was decreased significantly in formic acid and distilled water sprayed pork loin samples. From these results, it can be considered that application of combination of washing of formic acid and water in the washing step of pig slaughter may be helpful to control the proliferation of E. coli.
Polymorphisms of LEP, LGB and PRLR in water buffalo
Seong, Jiyeon ; Kong, Hong Sik ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 577~581
DOI : 10.7744/cnujas.2012.39.4.577
The polymorphisms of several genes including Leptin (LEP), beta-lactoglobulin (LGB) and Prolactin receptor (PRLR) have been shown to affect milk composition traits in dairy cattle. But, the effects of these polymorphisms on the milk traits of Philippine water buffalo are still unclear. In the Philippines, buffalo are the major milk producers most of which are the Philippine carabao (PC), the American Murrah Buffalo (AMB) and Bulgarian Murrah Buffalo (BMB). The LEP, LGB and PRLR genes are considered to be associated with milk production traits. The objective of the present study was to identify the single nucleotide polymorphisms (SNPs) in the LEP, LGB and PRLR genes of PC, AMB and BMB and to investigate the effect of the SNPs on milk production traits in these buffalo. Genetic polymorphisms were screened by DNA sequencing and 12 SNPs were detected in BMB; 5 SNPs were in LEP exon3 region (G14227A, G14343A, T14502C, C14526T, G14603A); 5 SNPs were in LGB exon 2 region (G1861C, A1900G, G1901T, T1948C, G1949A); 2 SNPs were in PRLR exon 6 (T59047C, T59109C). Also, 12 polymorphism sites between cattle and buffalo were identified. Our analysis of the association between SNPs and milk production traits should be useful in future studies of buffalo breeding to improve lactation performance.
Isolation and identification of antifungal compounds from Reynoutria elliptica
Hwang, Joo-Tae ; Park, Young-Sik ; Kim, Young-Shin ; Kim, Jin-Cheol ; Lim, Chi-Hwan ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 583~589
DOI : 10.7744/cnujas.2012.39.4.583
In the continued research on natural fungicides for the control of plant diseases by using plant-derived products, we found that Reynoutria elliptica. had a strong fungicidal activity against several plant pathogens. R. elliptica (3.2 kg) were extracted with 80% aq. MeOH and the concentrated extracted was partitioned with n-hexane, EtOAc, n-BuOH and
successively. The four layers were tested their disease control efficacies against 4 plant disease such as rise blast (RCB), tomato grey mold (TGM), tomato late blight (TLB), and barly powdery mildew (BPM). The n-hexane fraction was highly active showing over 95% control against TLB and BPM. and the EtOAc fraction was highly active showing over 95% control against RCB, TLB, and BPM. By using silica gel chromatography, MPLC, and HPLC, three compounds that were expected to have antifungal activity were isolated. Their chemical structures were elucidated as physcion, emodin, and emodie-8-O-glucoside by EI-MS and NMR spectroscopic analyses.
Application of electric pulsed power on fruit body production of edible and medicinal mushrooms
Ohga, Shoji ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 591~594
DOI : 10.7744/cnujas.2012.39.4.591
Effect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes, Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotus eryngii, Pleurotus abalonus, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 kV was directly charged to the substrate just before fructification. The effect of the pulsed power resulted to promote for 10 edible mushrooms fructification. The treatment especially stimulated the fructification on Pleurotus species.
Visitor perception survey on the Saemangeum reclamation district
Son, Jae-Gwon ; Kim, Seong-Su ; Choi, Jin-Kyu ; Jang, Dong-Heon ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 595~602
DOI : 10.7744/cnujas.2012.39.4.595
Main objective is to examine visitors the image, awareness of Saemangeum to examine its image, awareness, expected projects, and areas of improvement. This can establish measures for publicity and nation-wide participation. Main results are as following. First, visitors are broadly distributed to all age groups. Majority of visiting is travel and rate of returning visit is high as well. Second, the Saemangeum project is positively recognized and it is expected to be a prime development of the west coast in the future. Third, TV has significantly contributed to the visit to Saemangeum but the internet has larger effects in younger generations. Furthermore, rest and experience facilities, accommodations, and food items are major areas of improvement. Fourth, visitors have high expectations for tours, leisure, and ecosystems. Therefore, efforts should be made to become the Mecca of greed-development and cultural tourism. Fifth, many people replied that Saemangeum is an assistance to the national development but water quality management should be continued. In addition, the public participation should be made centrically cultural and economical participations.
Development of non-destructive pungency measurement technique for red-pepper powder produced in different domestic origins
Mo, Changyeun ; Lee, Kangjin ; Lim, Jong-Guk ; Kang, Sukwon ; Lee, Hyun-Dong ; Cho, Byoung-Kwan ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 603~612
DOI : 10.7744/cnujas.2012.39.4.603
In this research, the feasibility of non-destructive measurement technique of pungency measurement was investigated for the red-pepper powders produced in different domestic areas in South Korea. The near-infrared absorption spectra in the range of 1100 nm~2300 nm was used to measure capsaicinoids content in red-pepper powders by using a NIR spectroscopy equipped with Acousto-optic tunable filters (AOTF). Fourth three different red-pepper powders from 14 different locations were collected and separated in three different particle size (below 0.425 mm, 0.425~0.71 mm, 0.71~1.4 mm) for the spectral measurements. The partial least square regression (PLSR) models to predict the capsaicinoids content depends on particle size were developed with the measured spectra. The determinant coefficients and standard errors of the developed models for the red-pepper powders of below 0.425 mm, 0.425~0.71 mm, and 0.71~1.4 mm were in the range of 0.859~0.887 and 12.90~12.99 mg/100 g, respectively. The PLS model with the pretreatment of Standard Normal Variate (SNV) for the red-pepper powders below 1.4 mm particle size showed the best performance with the determinant coefficient of 0.844 and the standard error of 14.63 mg/100 g.
The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit
Choi, Sunmi ; Kim, Giyoung ; Om, Aeson ; Moon, Jihea ; Park, Saetbyeol ; Lee, Sangdae ; Kim, Hyuk Joo ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 613~618
DOI : 10.7744/cnujas.2012.39.4.613
This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.
Research on the corrent status and prospects of the rural industry development projects
Park, Dong-Jin ;
Korean Journal of Agricultural Science, volume 39, issue 4, 2012, Pages 619~628
DOI : 10.7744/cnujas.2012.39.4.619
Promoting Rural industry development projects with the support of the Ministry for Food, Agriculture, Forestry and Fisheries had elapsed about 5 years old. Agency selected 30 municipalities they annually promoting business, its performance will be examined. The alternative analysis of the business problem and seeks to provide policy direction. Superior rural enterprises to build business through fostering rural industry than what the system of rural industries in the region to want to foster entrepreneurship, 6th industrialization area industrial structure for each of the items, and not continue to make policy judgments about the point of needis. Therefore, in this paper, so far, through the analysis of the performance of alternatives by sector presented.